公开(公告)号：US20050244864A1

公开(公告)日：2005-11-03

申请号：US11085792

申请日：2005-03-21

申请人： Alan Kleinfeld ,  Andrew Huber ,  James Kampf ,  Thomas Kwan ,  Baolong Zhu

发明人： Alan Kleinfeld ,  Andrew Huber ,  James Kampf ,  Thomas Kwan ,  Baolong Zhu

IPC分类号： C12P19/34 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/58 ,  G01N33/72 ,  G01N33/92

CPC分类号： C12P21/02 ,  G01N33/533 ,  G01N33/582 ,  G01N33/728

摘要： A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.

摘要翻译： 描述了探针的高通量筛选方法。 这些探针可用于表征和测量流体样品中未结合的代谢物，特别是未结合的游离脂肪酸的表征和测量。 通过实践所公开的发明，可以确定可以用于确定个体相对于诸如中风，心脏病和癌症的疾病风险的个体的未结合代谢物的概况。

公开(公告)号：US20060257938A1

公开(公告)日：2006-11-16

申请号：US11430083

申请日：2006-05-08

申请人： Alan Kleinfeld ,  James Kampf

发明人： Alan Kleinfeld ,  James Kampf

IPC分类号： G01N33/53

CPC分类号： G01N33/574 ,  G01N33/582 ,  G01N33/92 ,  G01N2500/00 ,  G01N2500/02 ,  G01N2800/042

摘要： Methods are disclosed to screen for drugs that interfere with the binding between a specific lipid-binding protein and a selected ligand. Successful candidates have potential in disease treatments, particularly diabetes and some forms of cancer.

摘要翻译： 公开了筛选干扰特异性脂质结合蛋白与选定配体之间的结合的药物的方法。 成功的候选人在疾病治疗方面具有潜力，特别是糖尿病和某些形式的癌症。

公开(公告)号：US20160282368A1

公开(公告)日：2016-09-29

申请号：US15037910

申请日：2014-12-02

申请人： Alan Kleinfeld

发明人： Alan Kleinfeld ,  Andrew Huber

IPC分类号： G01N33/92 ,  G01N33/58

摘要： The invention relates to determination of the profile of unbound free fatty acids (FFAu) in biological samples, such as human and animal blood specimens and plant and animal oils, by measuring the fluorescence response of sets of different fluorescently labeled fatty acid binding proteins (probes) that undergo a change in fluorescence ratio at 2 wavelengths upon binding an FFAu. Use of these profiles in human and animal disease, in basic research, in drug development and in industrial uses is disclosed.

摘要翻译： 本发明涉及通过测量不同荧光标记的脂肪酸结合蛋白（探针）的荧光响应来确定生物样品中的未结合游离脂肪酸（FFAu）在人和动物血液标本以及植物和动物油中的分布 ）在结合FFAu时经历2波长的荧光比变化。 公开了在人畜动物疾病，基础研究，药物开发和工业用途中使用这些资料。

公开(公告)号：US07879558B2

公开(公告)日：2011-02-01

申请号：US11841480

申请日：2007-08-20

申请人： Alan Kleinfeld

发明人： Alan Kleinfeld

IPC分类号： G01N33/53

CPC分类号： G01N33/6893 ,  G01N33/92 ,  G01N2800/2871 ,  G01N2800/324 ,  G01N2800/52 ,  Y10S436/805 ,  Y10S436/815

摘要： This invention relates to the diagnosis and monitoring of ischemia, including but not limited to myocardial and cerebral ischemia, by measuring the concentration of molecules that do not originate from the ischemic tissue but whose concentration in the blood and other fluids changes as a consequence of the ischemic state.

摘要翻译： 本发明涉及诊断和监测缺血，包括但不限于心肌和脑缺血，通过测量不是源自缺血组织但是其血液和其它液体中的浓度由于 缺血状态。

公开(公告)号：US20050068534A1

公开(公告)日：2005-03-31

申请号：US10670958

申请日：2003-09-25

申请人： Alan Kleinfeld ,  Pavel Vodkin

发明人： Alan Kleinfeld ,  Pavel Vodkin

IPC分类号： G01N21/64

CPC分类号： G01N21/645 ,  G01N2021/6421

摘要： A method and apparatus for determining the concentration of molecules or atoms by measuring the ratio of two fluorescence signals. A sample having fluorescent molecules is exposed to radiation or excitation energy from a first source, which can be a broadband light source. The fluorescence of the sample is detected at two different wavelengths. The concentration of specific molecules or atoms within the sample is determined using the ratio of the two fluorescence signals. Fluorescent molecules can be bound to a human serum or plasma sample to allow determination of the concentration of unbound free fatty acids in the sample.

摘要翻译： 通过测量两个荧光信号的比例来确定分子或原子的浓度的方法和装置。 具有荧光分子的样品暴露于来自可以是宽带光源的第一源的辐射或激发能。 在两个不同的波长处检测样品的荧光。 使用两个荧光信号的比例来确定样品中特定分子或原子的浓度。 荧光分子可以结合人血清或血浆样品，以测定样品中未结合游离脂肪酸的浓度。

公开(公告)号：US10705101B2

公开(公告)日：2020-07-07

申请号：US15037910

申请日：2014-12-02

申请人： Alan Kleinfeld

发明人： Alan Kleinfeld ,  Andrew Huber

IPC分类号： G01N33/92 ,  G01N33/58

摘要： The invention relates to determination of the profile of unbound free fatty acids (FFAu) in biological samples, such as human and animal blood specimens and plant and animal oils, by measuring the fluorescence response of sets of different fluorescently labeled fatty acid binding proteins (probes) that undergo a change in fluorescence ratio at 2 wavelengths upon binding an FFAu. Use of these profiles in human and animal disease, in basic research, in drug development and in industrial uses is disclosed.

公开(公告)号：US20080004210A1

公开(公告)日：2008-01-03

申请号：US11841480

申请日：2007-08-20

申请人： Alan Kleinfeld

发明人： Alan Kleinfeld

IPC分类号： G01N33/92 ,  A61K38/28 ,  A61K38/38 ,  A61P9/10 ,  G01N33/53

CPC分类号： G01N33/6893 ,  G01N33/92 ,  G01N2800/2871 ,  G01N2800/324 ,  G01N2800/52 ,  Y10S436/805 ,  Y10S436/815

摘要： This invention relates to the diagnosis and monitoring of ischemia, including but not limited to myocardial and cerebral ischemia, by measuring the concentration of molecules that do not originate from the ischemic tissue but whose concentration in the blood and other fluids changes as a consequence of the ischemic state.

摘要翻译： 本发明涉及诊断和监测缺血，包括但不限于心肌和脑缺血，通过测量不是源自缺血组织但是其血液和其它液体中的浓度由于 缺血状态。

公开(公告)号：US07202089B2

公开(公告)日：2007-04-10

申请号：US10243565

申请日：2002-09-13

申请人： Alan Kleinfeld

发明人： Alan Kleinfeld

IPC分类号： G01N33/92

CPC分类号： G01N33/92 ,  Y10S435/968

摘要： A method is described for the early detection of stroke which uses a reagent which includes a fluorescently modified fatty acid binding protein. A fluorescence difference is noted between the bound and unbound condition. Elevated levels of unbound free fatty acids from blood are used as indicators of stroke.

摘要翻译： 描述了使用包含荧光改性的脂肪酸结合蛋白的试剂的中风早期检测方法。 注意到结合和未结合条件之间的荧光差异。 来自血液的未结合游离脂肪酸水平升高被用作中风的指标。

公开(公告)号：US20100292086A1

公开(公告)日：2010-11-18

申请号：US12863765

申请日：2009-01-27

申请人： Alan Kleinfeld

发明人： Alan Kleinfeld

IPC分类号： C40B30/00 ,  G01N33/566

CPC分类号： G01N21/6428

摘要： Methods for discovering compounds that modulate metabolite transport across cell membranes are disclosed. Fluorescently labeled protein probes which have an affinity for an unbound metabolite are trapped in cells. The influx of the unbound metabolite into the cell causes a change in the fluorescence of the protein probe. The ability of test compounds to modulate the fluorescent signal is used to screen for compounds which affect transport of the unbound metabolite. In an example, the unbound metabolite is an unbound free fatty acid and the probe is a fatty acid binding protein.

摘要翻译： 公开了发现调节跨细胞膜的代谢物转运的化合物的方法。 对未结合的代谢物具有亲和性的荧光标记的蛋白质探针被捕获在细胞中。 未结合的代谢物进入细胞会导致蛋白质探针的荧光发生变化。 测试化合物调节荧光信号的能力用于筛选影响未结合代谢物运输的化合物。 在一个实例中，未结合的代谢物是未结合的游离脂肪酸，探针是脂肪酸结合蛋白。

公开(公告)号：US07262017B2

公开(公告)日：2007-08-28

申请号：US10243566

申请日：2002-09-13

申请人： Alan Kleinfeld

发明人： Alan Kleinfeld

IPC分类号： G01N33/53

CPC分类号： G01N33/6893 ,  G01N33/92 ,  G01N2800/2871 ,  G01N2800/324 ,  G01N2800/52 ,  Y10S436/805 ,  Y10S436/815

摘要： This invention relates to the diagnosis and monitoring of ischemia, including but not limited to myocardial and cerebral ischemia, by measuring the concentration of molecules that do not originate from the ischemic tissue but whose concentration in the blood and other fluids changes as a consequence of the ischemic state.

摘要翻译： 本发明涉及诊断和监测缺血，包括但不限于心肌和脑缺血，通过测量不是源自缺血组织但是其血液和其它液体中的浓度由于 缺血状态。