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公开(公告)号：US20050244864A1
公开(公告)日：2005-11-03
申请号：US11085792
申请日：2005-03-21
申请人： Alan Kleinfeld , Andrew Huber , James Kampf , Thomas Kwan , Baolong Zhu
发明人： Alan Kleinfeld , Andrew Huber , James Kampf , Thomas Kwan , Baolong Zhu
CPC分类号： C12P21/02 , G01N33/533 , G01N33/582 , G01N33/728
摘要： A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.
摘要翻译： 描述了探针的高通量筛选方法。 这些探针可用于表征和测量流体样品中未结合的代谢物，特别是未结合的游离脂肪酸的表征和测量。 通过实践所公开的发明，可以确定可以用于确定个体相对于诸如中风，心脏病和癌症的疾病风险的个体的未结合代谢物的概况。
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公开(公告)号：US20100062948A1
公开(公告)日：2010-03-11
申请号：US12447400
申请日：2007-10-26
CPC分类号： G01N33/92 , G01N33/728
摘要： Methods of determining levels of unbound metabolites are disclosed. Probes derived from fatty acid binding protein muteins are described that bind preferentially to a number of unbound metabolites including oleate, stearate, linoleate, palmitate, arachidonate and unconjugated bilirubin. A profile for a patient is determined using one or more of the described probes. The profile is useful in diagnosis of disease, particularly myocardial infarction, non-alcoholic fatty liver disease (NAFLD), diabetes, stroke, sepsis and neonatal jaundice. The responses of multiple probes to a test sample are used to classify the degree of acute coronary syndrome by comparison to multi-probe profiles generated from unstable angina, non ST elevation myocardial infarction, and ST elevation myocardial infarction.
摘要翻译： 公开了确定未结合代谢物水平的方法。 描述了衍生自脂肪酸结合蛋白质突变蛋白的探针，其优先结合许多未结合的代谢物，包括油酸盐，硬脂酸盐，亚油酸盐，棕榈酸盐，花生四烯酸盐和未缀合的胆红素。 使用所描述的一个或多个探针确定患者的轮廓。 该资料可用于诊断疾病，特别是心肌梗死，非酒精性脂肪性肝病（NAFLD），糖尿病，中风，败血症和新生儿黄疸。 将多个探针对测试样品的反应用于通过与由不稳定型心绞痛，非ST段抬高型心肌梗死和ST段抬高型心肌梗死产生的多探针分布进行比较来分类急性冠状动脉综合征的程度。
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公开(公告)号：US08466090B2
公开(公告)日：2013-06-18
申请号：US12535617
申请日：2009-08-04
CPC分类号： C12P21/02 , G01N33/533 , G01N33/582 , G01N33/728
摘要： A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.
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公开(公告)号：US09529003B2
公开(公告)日：2016-12-27
申请号：US14238144
申请日：2012-08-24
CPC分类号： G01N33/728 , C07K14/47 , G01N21/6428 , G01N33/533 , G01N33/582 , G01N33/92 , G01N2800/52 , Y10T436/146666
摘要： Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample.
摘要翻译： 描述了荧光标记并在结合胆红素时发生荧光指数变化的蛋白质的鉴定和使用。 公开了在半胱氨酸或赖氨酸残基上标记的探针，以及用两种不同的荧光团在半胱氨酸和赖氨酸标记的探针。 这些探针可用于测定流体样品中未结合的胆红素水平。
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5.发明授权Development and use of cysteine-labeled fluorescent probes of unbound analytes 有权开发和使用未结合分析物的半胱氨酸标记的荧光探针公开(公告)号：US09134317B2
公开(公告)日：2015-09-15
申请号：US12810505
申请日：2009-01-16
摘要： A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease.
摘要翻译： 描述了在结合未结合的分析物时在半胱氨酸残基处荧光标记并在2个波长下发生荧光比变化的高通量发现的方法。 公开了在半胱氨酸残基上标记的探针，以及用两种不同荧光团在半胱氨酸和赖氨酸标记的探针。 这些探针可用于流体样品中疏水物质的表征和测量，特别是未结合游离脂肪酸的表征和测量。 可以确定可以用于确定个体相对于疾病风险的个体的未结合游离脂肪酸的概况。
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公开(公告)号：US20150044692A1
公开(公告)日：2015-02-12
申请号：US14238144
申请日：2012-08-24
CPC分类号： G01N33/728 , C07K14/47 , G01N21/6428 , G01N33/533 , G01N33/582 , G01N33/92 , G01N2800/52 , Y10T436/146666
摘要： Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample.
摘要翻译： 描述了荧光标记并在结合胆红素时发生荧光指数变化的蛋白质的鉴定和使用。 公开了在半胱氨酸或赖氨酸残基上标记的探针，以及用两种不同的荧光团在半胱氨酸和赖氨酸标记的探针。 这些探针可用于测定流体样品中未结合的胆红素水平。
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公开(公告)号：US09164109B2
公开(公告)日：2015-10-20
申请号：US12447400
申请日：2007-10-26
CPC分类号： G01N33/92 , G01N33/728
摘要： Methods of determining levels of unbound metabolites are disclosed. Probes derived from fatty acid binding protein muteins are described that bind preferentially to a number of unbound metabolites including oleate, stearate, linoleate, palmitate, arachidonate and unconjugated bilirubin. A profile for a patient is determined using one or more of the described probes. The profile is useful in diagnosis of disease, particularly myocardial infarction, non-alcoholic fatty liver disease (NAFLD), diabetes, stroke, sepsis and neonatal jaundice. The responses of multiple probes to a test sample are used to classify the degree of acute coronary syndrome by comparison to multi-probe profiles generated from unstable angina, non ST elevation myocardial infarction, and ST elevation myocardial infarction.
摘要翻译： 公开了确定未结合代谢物水平的方法。 描述了衍生自脂肪酸结合蛋白质突变蛋白的探针，其优先结合许多未结合的代谢物，包括油酸盐，硬脂酸盐，亚油酸盐，棕榈酸盐，花生四烯酸盐和未缀合的胆红素。 使用所描述的一个或多个探针确定患者的轮廓。 该资料可用于诊断疾病，特别是心肌梗死，非酒精性脂肪性肝病（NAFLD），糖尿病，中风，败血症和新生儿黄疸。 将多个探针对测试样品的反应用于通过与由不稳定型心绞痛，非ST段抬高型心肌梗死和ST段抬高型心肌梗死产生的多探针分布进行比较来分类急性冠状动脉综合征的程度。
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8.发明申请公开(公告)号：US20100298162A1
公开(公告)日：2010-11-25
申请号：US12810505
申请日：2009-01-16
摘要： A method for high throughput discovery of proteins fluorescently labeled at a cysteine residue and that undergo a change in fluorescence ratio at 2 wavelengths upon binding an unbound analyte is described. Probes are disclosed which are labeled at a cysteine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for characterization and measurement of hydrophobic species in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. A profile of unbound free fatty acids can be determined for an individual which can be used to determine the individual's relative risk for disease.
摘要翻译： 描述了在结合未结合的分析物时在半胱氨酸残基处荧光标记并在2个波长下发生荧光比变化的高通量发现的方法。 公开了在半胱氨酸残基上标记的探针，以及用两种不同荧光团在半胱氨酸和赖氨酸标记的探针。 这些探针可用于流体样品中疏水物质的表征和测量，特别是未结合游离脂肪酸的表征和测量。 可以确定可以用于确定个体相对于疾病风险的个体的未结合游离脂肪酸的概况。
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公开(公告)号：US07601510B2
公开(公告)日：2009-10-13
申请号：US11085792
申请日：2005-03-21
IPC分类号： G01N33/567 , G01N33/543 , G01N33/44 , C07K14/00
CPC分类号： C12P21/02 , G01N33/533 , G01N33/582 , G01N33/728
摘要： A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.
摘要翻译： 描述了探针的高通量筛选方法。 这些探针可用于表征和测量流体样品中未结合的代谢物，特别是未结合的游离脂肪酸的表征和测量。 通过实践所公开的发明，可以确定可以用于确定个体相对于诸如中风，心脏病和癌症的疾病风险的个体的未结合代谢物的概况。
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公开(公告)号：US20090221501A1
公开(公告)日：2009-09-03
申请号：US12371825
申请日：2009-02-16
申请人： Timothy D. Hey , Amanda D. Schleper , Scott A. Bevan , Scott B. Bintrim , Jon C. Mitchell , Ze Sheng Li , Weiting Ni , Baolong Zhu , Donald J. Merlo , Patricia C. Apel-Birkhold , Thomas Meade
发明人： Timothy D. Hey , Amanda D. Schleper , Scott A. Bevan , Scott B. Bintrim , Jon C. Mitchell , Ze Sheng Li , Weiting Ni , Baolong Zhu , Donald J. Merlo , Patricia C. Apel-Birkhold , Thomas Meade
CPC分类号： C07K14/195 , A01N37/46 , A01N63/02 , C07K14/24 , C12N15/8286 , Y02A40/162
摘要： The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. (As one skilled in the art knows, Xenorhabdus toxin proteins tend to be more desirable for controlling lepidopterans while Photorhabdus toxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.
摘要翻译： 本发明涉及令人惊奇的发现，可以从Xenorhabdus，Photorhabdus和Paenibacillus获得的毒素复合物（TC）蛋白质彼此互换使用。 在本发明的特别优选的实施方案中，“独立”TC蛋白（例如，来自Photorhabdus，Xenorhabdus或Paenibacillus的例如）的毒性由一种或多种衍生自以下的源生物体的TC蛋白“增强剂”增强 来源于毒素的不同属。 本领域技术人员将会认识到本公开的益处，这具有广泛的含义并扩大了现在将认识到各种类型的TC蛋白质具有的效用范围。 最重要的优点之一是，本领域技术人员现在将能够使用单一增强剂来增强独立的Xenorhabdus蛋白毒素的活性以及独立的Photorhabdus蛋白毒素。 （如本领域技术人员所知，Xenorhabdus毒素蛋白质倾向于更好地控制鳞翅目，而Photorhabdus毒素蛋白质倾向于更有利于控制鞘翅目）。这减少了需要表达的基因数量和转化事件 一个转基因植物，以实现更广泛的目标害虫的有效控制。 异源TC蛋白的某些优选组合也在本文中公开。 受益于本发明的本发明的其它目的，优点和特征对本领域技术人员而言是显而易见的。
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