公开(公告)号：US20050244864A1

公开(公告)日：2005-11-03

申请号：US11085792

申请日：2005-03-21

申请人： Alan Kleinfeld ,  Andrew Huber ,  James Kampf ,  Thomas Kwan ,  Baolong Zhu

发明人： Alan Kleinfeld ,  Andrew Huber ,  James Kampf ,  Thomas Kwan ,  Baolong Zhu

IPC分类号： C12P19/34 ,  C12P21/02 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/58 ,  G01N33/72 ,  G01N33/92

CPC分类号： C12P21/02 ,  G01N33/533 ,  G01N33/582 ,  G01N33/728

摘要： A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.

摘要翻译： 描述了探针的高通量筛选方法。 这些探针可用于表征和测量流体样品中未结合的代谢物，特别是未结合的游离脂肪酸的表征和测量。 通过实践所公开的发明，可以确定可以用于确定个体相对于诸如中风，心脏病和癌症的疾病风险的个体的未结合代谢物的概况。

公开(公告)号：US10705101B2

公开(公告)日：2020-07-07

申请号：US15037910

申请日：2014-12-02

申请人： Alan Kleinfeld

发明人： Alan Kleinfeld ,  Andrew Huber

IPC分类号： G01N33/92 ,  G01N33/58

摘要： The invention relates to determination of the profile of unbound free fatty acids (FFAu) in biological samples, such as human and animal blood specimens and plant and animal oils, by measuring the fluorescence response of sets of different fluorescently labeled fatty acid binding proteins (probes) that undergo a change in fluorescence ratio at 2 wavelengths upon binding an FFAu. Use of these profiles in human and animal disease, in basic research, in drug development and in industrial uses is disclosed.

公开(公告)号：US20160282368A1

公开(公告)日：2016-09-29

申请号：US15037910

申请日：2014-12-02

申请人： Alan Kleinfeld

发明人： Alan Kleinfeld ,  Andrew Huber

IPC分类号： G01N33/92 ,  G01N33/58

摘要： The invention relates to determination of the profile of unbound free fatty acids (FFAu) in biological samples, such as human and animal blood specimens and plant and animal oils, by measuring the fluorescence response of sets of different fluorescently labeled fatty acid binding proteins (probes) that undergo a change in fluorescence ratio at 2 wavelengths upon binding an FFAu. Use of these profiles in human and animal disease, in basic research, in drug development and in industrial uses is disclosed.

摘要翻译： 本发明涉及通过测量不同荧光标记的脂肪酸结合蛋白（探针）的荧光响应来确定生物样品中的未结合游离脂肪酸（FFAu）在人和动物血液标本以及植物和动物油中的分布 ）在结合FFAu时经历2波长的荧光比变化。 公开了在人畜动物疾病，基础研究，药物开发和工业用途中使用这些资料。

公开(公告)号：US20070035900A1

公开(公告)日：2007-02-15

申请号：US11202275

申请日：2005-08-11

申请人： Andrew Huber ,  Ciaran Brennan ,  Paul Dunn ,  Scott Gould ,  Lin Lin ,  Erich Schanzenbach

发明人： Andrew Huber ,  Ciaran Brennan ,  Paul Dunn ,  Scott Gould ,  Lin Lin ,  Erich Schanzenbach

IPC分类号： H02H9/00

CPC分类号： G06F17/5068

摘要： An integrated system and method to achieve ESD robustness on an integrated circuit (IC) in a fully automated ASIC design environment is described. Electrical characteristics and electrical limits on the power network are translated to power route region constraints for each chip input/output (I/O) cell. Electrical limits on the signal network are translated into signal route region constraints for each chip I/O cell. These constraints are passed on to an I/O floorplanner (automatic placer of I/O cells) that analyzes trade-offs between these constraints. For I/O cells that can not be placed to satisfy both power and signal region constraints, the I/O floorplanner utilizes the knowledge of alternative power distribution structures to group I/Os and create local power grid structures that have the effect of relaxing the power region constraints. Instructions for creating these local power grid structures are passed on to the automatic power routing tool.

摘要翻译： 描述了在完全自动化ASIC设计环境中实现集成电路（IC）上ESD稳定性的集成系统和方法。 电力网络上的电气特性和电气限制被转换为每个芯片输入/输出（I / O）单元的功率路由区域约束。 信号网络上的电气限制被转换为每个芯片I / O单元的信号路由区域约束。 这些约束被传递到分析这些限制之间的权衡的I / O平面布局（I / O单元的自动放置器）。 对于不能放置以满足功率和信号区域约束的I / O单元，I / O平面布置器利用替代功率分配结构的知识来分组I / O，并创建具有放松效果的局部电网结构 功率区域约束。 创建这些局部电网结构的说明将传递给自动电力布线工具。