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    Apparatus for biological sample preparation and analysis
    2.
    发明授权
    Apparatus for biological sample preparation and analysis 失效
    用于生物样品制备和分析的装置

    公开(公告)号：US06692702B1

    公开(公告)日：2004-02-17

    申请号：US09611847

    申请日：2000-07-07

    申请人： Alexander Burshteyn ,  John W. Joubran ,  Nazle Kuylen ,  Frank J. Lucas

    发明人： Alexander Burshteyn ,  John W. Joubran ,  Nazle Kuylen ,  Frank J. Lucas

    IPC分类号： G01N134

    CPC分类号： G01N1/4077 ,  B01D61/147 ,  B01D61/18 ,  B01D61/20 ,  B01D63/02 ,  Y10T436/25375 ,  Y10T436/255

    摘要： A method for utilizing a filtration device for removing interferants from a sample containing cells in an automated apparatus is disclosed. The filtration device includes a microporous hollow fiber membrane having a plurality of pores sized to retain cells while allowing smaller diameter interferants to pass through the membrane. The apparatus also includes a means for of moving the sample from a sample container to and from the filtration device. The disclosed method utilizes a vacuum source to aspirate the sample into a lumen of the hollow fiber membrane so that the sample is retained in the lumen space until expelled into an analysis container or transported to an analyzer.

    摘要翻译： 公开了一种利用过滤装置从自动化装置中含有细胞的样品中除去干扰物的方法。 过滤装置包括具有多个孔的微孔中空纤维膜，其尺寸设计成保持细胞同时允许较小直径的干燥剂通过膜。 该设备还包括用于将样品从样品容器移动到过滤装置和从过滤装置移动的装置。 所公开的方法利用真空源将样品吸入中空纤维膜的内腔，使得样品保留在管腔空间中，直到排出到分析容器中或运送到分析器中。

    In situ use of gelatin in the preparation of uniform ferrite particles
    4.
    发明授权
    In situ use of gelatin in the preparation of uniform ferrite particles 失效
    原位使用明胶制备均匀的铁氧体颗粒

    公开(公告)号：US5062991A

    公开(公告)日：1991-11-05

    申请号：US532434

    申请日：1990-06-04

    申请人： Olavi Siiman ,  Alexander Burshteyn

    发明人： Olavi Siiman ,  Alexander Burshteyn

    IPC分类号： B01J13/06 ,  B01J13/00 ,  B03C1/01 ,  H01F1/06 ,  H01F1/26 ,  H01F1/44

    CPC分类号： B01J13/0013 ,  B03C1/01 ,  H01F1/061 ,  H01F1/445 ,  Y10T428/2991

    摘要： A method is described for the preparation of uniform colloidal particles of ferrites, containing manganese (II), zinc(II), barium(II), iron(II), cobalt(II), or nickel(II), or a mixture of manganese(II) and zinc(II), at a relatively low temperature in the presence of a gelatin solution which acts as a support vehicle for the nucleation and growth of colloidal particles of metal oxide and for dispersion as separate single particles.

    摘要翻译： 描述了制备含有锰（II），锌（II），钡（II），铁（II），钴（II）或镍（II））的铁素体的均匀胶态粒子的方法，或者 锰（II）和锌（II）在相对较低的温度下，在明胶溶液的存在下，其作为用于金属氧化物的胶体颗粒的成核和生长并用作分散的单个颗粒的载体载体。

    Ligand-aminodextran-marker conjugates and uses thereof
    5.
    发明授权
    Ligand-aminodextran-marker conjugates and uses thereof 失效
    配体 - 氨基葡聚糖标记共轭物及其用途

    公开(公告)号：US06387622B1

    公开(公告)日：2002-05-14

    申请号：US09403919

    申请日：1999-10-27

    申请人： Olavi Siiman ,  Alexander Burshteyn ,  Ravindra Mylvaganam ,  Robert Raynor ,  Patricia Roth ,  Cecilia Smith ,  Julie Wilkinson

    发明人： Olavi Siiman ,  Alexander Burshteyn ,  Ravindra Mylvaganam ,  Robert Raynor ,  Patricia Roth ,  Cecilia Smith ,  Julie Wilkinson

    IPC分类号： G01N3368

    CPC分类号： G01N33/533 ,  G01N33/566 ,  G01N33/56977 ,  Y10S436/80 ,  Y10S436/805

    摘要： Ligand-aminodextran-(phycobiliprotein or tandem dye) conjugates useful for detection of a desired target biological material by providing an enhanced fluorescent signal are described. Also described is a method for a single-measurement quantification of multiple populations of cells based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked to an aminodextran to a fluorescent phycobiliprotein or tandem dye.

    摘要翻译： 描述了通过提供增强的荧光信号用于检测所需目标生物材料的配体 - 氨基葡聚糖 - （藻胆蛋白或串联染料）缀合物。 还描述了基于不同对细胞群的标记单个测量定量细胞群的方法，每对细胞群含有相互排斥的细胞受体，其以与每个受体的标记配体基本相似的受体密度表达。 一个细胞群体用能够结合第一细胞表面受体的配体标记，该配体直接与荧光藻胆蛋白或串联染料缀合; 并且用能够结合第二细胞表面受体的配体标记第二细胞群体，该配体与氨基葡聚糖交联至荧光藻胆蛋白或串联染料。

    Methods of enumerating receptor molecules for specific binding partners on formed bodies and in solution
    7.
    发明授权
    Methods of enumerating receptor molecules for specific binding partners on formed bodies and in solution 失效
    列举形成体和溶液中特异性结合配偶体的受体分子的方法

    公开(公告)号：US5814468A

    公开(公告)日：1998-09-29

    申请号：US624014

    申请日：1996-03-27

    申请人： Olavi Siiman ,  Alexander Burshteyn ,  Orlando Concepcion ,  Meryl Forman

    发明人： Olavi Siiman ,  Alexander Burshteyn ,  Orlando Concepcion ,  Meryl Forman

    IPC分类号： G01N33/566 ,  G01N33/533 ,  C12Q1/70

    CPC分类号： G01N33/566

    摘要： This invention relates generally to using fluorescent markers (labelled antibodies) and flow cytometry to enumerate the average number of receptors (antigen) on formed bodies (cells) in whole blood, and to evaluate the specific binding constant of the marker for the particular receptors. Mean channel fluorescence intensities of equilibrated marker-cell suspension mixtures, total concentrations of marker, and targeted cell counts obtained by established procedures are used to complete the analyses. The invention further describes a competitive binding assay between marker and unlabelled antibody for receptors shed from the surface of formed bodies into solution and receptors on the formed bodies to analyze for receptors in solution. Moreover, competitive binding assays between marker and unlabelled antibody for receptors on formed bodies in whole blood are described for determination of relative, specific, and average binding constants of unlabelled/labelled antibody for targeted receptors.

    摘要翻译： 本发明一般涉及使用荧光标记（标记抗体）和流式细胞术来列举全血中成体体（细胞）上的受体（抗原）的平均数目，并评估特定受体的标记物的特异结合常数。 平衡的标记 - 细胞悬浮液混合物的平均通道荧光强度，通过建立的程序获得的标记物的总浓度和靶细胞计数用于完成分析。 本发明进一步描述了标记物与未标记抗体之间的竞争性结合测定法，用于从形成体表面转移到溶液中的受体和形成体上的受体以分析溶液中的受体。 此外，描述了用于确定未标记/标记的靶向受体抗体的相对，特异性和平均结合常数的标记物和未标记抗体之间在全血中形成体上的受体的竞争结合测定。

    Surface treatments to impart hydrophilicity
    8.
    发明授权
    Surface treatments to impart hydrophilicity 失效
    表面处理赋予亲水性

    公开(公告)号：US5312873A

    公开(公告)日：1994-05-17

    申请号：US711673

    申请日：1991-06-06

    申请人： Harry P. Gregor ,  Alexander Burshteyn ,  Leonard T. Hodgins ,  John Kassotis ,  Edgar Samuelson

    发明人： Harry P. Gregor ,  Alexander Burshteyn ,  Leonard T. Hodgins ,  John Kassotis ,  Edgar Samuelson

    IPC分类号： B01D67/00 ,  B01D69/12 ,  B01D71/42 ,  C08F8/00 ,  C08J7/12 ,  C08F8/04

    CPC分类号： B01D67/0093 ,  B01D69/125 ,  B01D71/42 ,  C08F8/00 ,  C08J7/12 ,  B01D2323/30

    摘要： Water insoluble polymers are treated to place on their exposed surfaces the pendant alkyl imidocarbonyl (amide) groups to impart a high degree of hydrophilicity to that surface. Nitrile-containing polymers or copolymers in the form of insoluble films, filters or membranes are treated to convert a substantial fraction of the surface nitrile groups into the corresponding amide by the use of hydrogen peroxide or concentrated acids. The surface is rendered hydrophilic and resistant to fouling to the extent that even proteins are not denatured thereupon. The surface may also be converted to substituted amides by subsequent reactions to impart to it other functionalities or to serve as the basis for coupling reactions. Other polymers capable of surface treatment to produce pendant amidocarbonyl groups include ones containing pendant esters which on cleavage of the oxygen-carbon bond give pendant alcohol groups. These are converted by cyanoethylation to form the pendant nitrile groups which can be converted to the amide. Other polymers which on hydrolysis give pendant carboxylic acids can be treated with ammonia to also produce pendant amide groups.

    摘要翻译： 处理水不溶性聚合物以在其暴露的表面上放置亚酰氨基羰基（酰胺）基团，以赋予该表面高度亲水性。 处理不溶性膜，过滤器或膜形式的含腈聚合物或共聚物，通过使用过氧化氢或浓缩酸将大部分表面腈基转化为相应的酰胺。 表面被赋予亲水性并且抗结垢至甚至蛋白质不变性的程度。 该表面也可以通过后续反应转化为取代的酰胺，以赋予其它功能或用作偶联反应的基础。 能够进行表面处理以产生酰亚胺基羰基的其它聚合物包括含有侧链酯的聚合物，其在氧 - 碳键裂解时产生侧链醇基。 这些通过氰乙基化反应形成可以转化成酰胺的侧基腈基。 可以用氨处理水解产生侧羧酸的其它聚合物，也可产生酰胺基。

    Surface treatments to impart hydrophilicity
    10.
    发明授权
    Surface treatments to impart hydrophilicity 失效
    表面处理赋予亲水性

    公开(公告)号：US5059659A

    公开(公告)日：1991-10-22

    申请号：US635421

    申请日：1991-01-03

    申请人： Harry P. Gregor ,  Alexander Burshteyn ,  Leonard T. Hodgins ,  John Kassotis ,  Edgar Samuelson

    发明人： Harry P. Gregor ,  Alexander Burshteyn ,  Leonard T. Hodgins ,  John Kassotis ,  Edgar Samuelson

    IPC分类号： B01D67/00 ,  B01D69/12 ,  B01D71/42 ,  C08F8/00 ,  C08J7/12

    CPC分类号： B01D67/0093 ,  B01D69/125 ,  B01D71/42 ,  C08F8/00 ,  C08J7/12 ,  B01D2323/30

    摘要： Water insoluble polymers are treated to place on their exposed surfaces the pendant alkyl imidocarbonyl (amide) groups to impart a high degree of hydrophilicity to that surface. Nitrile-containing polymers or copolymers in the form of insoluble films, filters or membranes are treated to convert a substantial fraction of the surface nitrile groups into the corresponding amide by the use of hydrogen peroxide or concentrated acids. The surface is rendered hydrophilic and resistant to fouling to the extent that even proteins are not denatured thereupon. The surface may also be converted to substituted amides by subsequent reactions to impart to it other functionalities or to serve as the basis for coupling reactions. Other polymers capable of surface treatment to produce pendant amidocarbonyl groups include ones containing pendant esters which on cleavage of the oxygen-carbon bond give pendant alcohol groups. These are converted by cyanoethylation to form the pendant nitrile groups which can be converted to the amide. Other polymers which on hydrolysis give pendant carboxylic acids can be treated with ammonia to also produce pendant amide groups.

    摘要翻译： 处理水不溶性聚合物以在其暴露的表面上放置亚酰氨基羰基（酰胺）基团，以赋予该表面高度亲水性。 处理不溶性膜，过滤器或膜形式的含腈聚合物或共聚物，通过使用过氧化氢或浓缩酸将大部分表面腈基转化为相应的酰胺。 表面被赋予亲水性并且抗结垢至甚至蛋白质不变性的程度。 该表面也可以通过后续反应转化为取代的酰胺，以赋予其它功能或用作偶联反应的基础。 能够进行表面处理以产生酰亚胺基羰基的其它聚合物包括含有侧链酯的聚合物，其在氧 - 碳键裂解时产生侧链醇基。 这些通过氰乙基化反应形成可以转化成酰胺的侧基腈基。 可以用氨处理水解产生侧羧酸的其它聚合物，也可产生酰胺基。