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20 条记录 (耗时 0.026 秒)

    In situ use of gelatin in the preparation of uniform ferrite particles
    3.
    发明授权
    In situ use of gelatin in the preparation of uniform ferrite particles 失效
    原位使用明胶制备均匀的铁氧体颗粒

    公开(公告)号：US5062991A

    公开(公告)日：1991-11-05

    申请号：US532434

    申请日：1990-06-04

    申请人： Olavi Siiman ,  Alexander Burshteyn

    发明人： Olavi Siiman ,  Alexander Burshteyn

    IPC分类号： B01J13/06 ,  B01J13/00 ,  B03C1/01 ,  H01F1/06 ,  H01F1/26 ,  H01F1/44

    CPC分类号： B01J13/0013 ,  B03C1/01 ,  H01F1/061 ,  H01F1/445 ,  Y10T428/2991

    摘要： A method is described for the preparation of uniform colloidal particles of ferrites, containing manganese (II), zinc(II), barium(II), iron(II), cobalt(II), or nickel(II), or a mixture of manganese(II) and zinc(II), at a relatively low temperature in the presence of a gelatin solution which acts as a support vehicle for the nucleation and growth of colloidal particles of metal oxide and for dispersion as separate single particles.

    摘要翻译： 描述了制备含有锰（II），锌（II），钡（II），铁（II），钴（II）或镍（II））的铁素体的均匀胶态粒子的方法，或者 锰（II）和锌（II）在相对较低的温度下，在明胶溶液的存在下，其作为用于金属氧化物的胶体颗粒的成核和生长并用作分散的单个颗粒的载体载体。

    Ligand-aminodextran-marker conjugates and uses thereof
    4.
    发明授权
    Ligand-aminodextran-marker conjugates and uses thereof 失效
    配体 - 氨基葡聚糖标记共轭物及其用途

    公开(公告)号：US06387622B1

    公开(公告)日：2002-05-14

    申请号：US09403919

    申请日：1999-10-27

    申请人： Olavi Siiman ,  Alexander Burshteyn ,  Ravindra Mylvaganam ,  Robert Raynor ,  Patricia Roth ,  Cecilia Smith ,  Julie Wilkinson

    发明人： Olavi Siiman ,  Alexander Burshteyn ,  Ravindra Mylvaganam ,  Robert Raynor ,  Patricia Roth ,  Cecilia Smith ,  Julie Wilkinson

    IPC分类号： G01N3368

    CPC分类号： G01N33/533 ,  G01N33/566 ,  G01N33/56977 ,  Y10S436/80 ,  Y10S436/805

    摘要： Ligand-aminodextran-(phycobiliprotein or tandem dye) conjugates useful for detection of a desired target biological material by providing an enhanced fluorescent signal are described. Also described is a method for a single-measurement quantification of multiple populations of cells based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked to an aminodextran to a fluorescent phycobiliprotein or tandem dye.

    摘要翻译： 描述了通过提供增强的荧光信号用于检测所需目标生物材料的配体 - 氨基葡聚糖 - （藻胆蛋白或串联染料）缀合物。 还描述了基于不同对细胞群的标记单个测量定量细胞群的方法，每对细胞群含有相互排斥的细胞受体，其以与每个受体的标记配体基本相似的受体密度表达。 一个细胞群体用能够结合第一细胞表面受体的配体标记，该配体直接与荧光藻胆蛋白或串联染料缀合; 并且用能够结合第二细胞表面受体的配体标记第二细胞群体，该配体与氨基葡聚糖交联至荧光藻胆蛋白或串联染料。

    Methods of enumerating receptor molecules for specific binding partners on formed bodies and in solution
    6.
    发明授权
    Methods of enumerating receptor molecules for specific binding partners on formed bodies and in solution 失效
    列举形成体和溶液中特异性结合配偶体的受体分子的方法

    公开(公告)号：US5814468A

    公开(公告)日：1998-09-29

    申请号：US624014

    申请日：1996-03-27

    申请人： Olavi Siiman ,  Alexander Burshteyn ,  Orlando Concepcion ,  Meryl Forman

    发明人： Olavi Siiman ,  Alexander Burshteyn ,  Orlando Concepcion ,  Meryl Forman

    IPC分类号： G01N33/566 ,  G01N33/533 ,  C12Q1/70

    CPC分类号： G01N33/566

    摘要： This invention relates generally to using fluorescent markers (labelled antibodies) and flow cytometry to enumerate the average number of receptors (antigen) on formed bodies (cells) in whole blood, and to evaluate the specific binding constant of the marker for the particular receptors. Mean channel fluorescence intensities of equilibrated marker-cell suspension mixtures, total concentrations of marker, and targeted cell counts obtained by established procedures are used to complete the analyses. The invention further describes a competitive binding assay between marker and unlabelled antibody for receptors shed from the surface of formed bodies into solution and receptors on the formed bodies to analyze for receptors in solution. Moreover, competitive binding assays between marker and unlabelled antibody for receptors on formed bodies in whole blood are described for determination of relative, specific, and average binding constants of unlabelled/labelled antibody for targeted receptors.

    摘要翻译： 本发明一般涉及使用荧光标记（标记抗体）和流式细胞术来列举全血中成体体（细胞）上的受体（抗原）的平均数目，并评估特定受体的标记物的特异结合常数。 平衡的标记 - 细胞悬浮液混合物的平均通道荧光强度，通过建立的程序获得的标记物的总浓度和靶细胞计数用于完成分析。 本发明进一步描述了标记物与未标记抗体之间的竞争性结合测定法，用于从形成体表面转移到溶液中的受体和形成体上的受体以分析溶液中的受体。 此外，描述了用于确定未标记/标记的靶向受体抗体的相对，特异性和平均结合常数的标记物和未标记抗体之间在全血中形成体上的受体的竞争结合测定。

    Simultaneous analyses of white blood cell subsets using multi-color, multi-intensity fluorescent markers in flow cytometry
    7.
    发明授权
    Simultaneous analyses of white blood cell subsets using multi-color, multi-intensity fluorescent markers in flow cytometry 失效
    在流式细胞术中使用多色多重荧光标记物同时分析白细胞亚群

    公开(公告)号：US5994089A

    公开(公告)日：1999-11-30

    申请号：US976031

    申请日：1997-11-21

    申请人： Olavi Siiman ,  Alexander Burshteyn ,  Julie Wilkinson ,  Ravindra Mylvaganam

    发明人： Olavi Siiman ,  Alexander Burshteyn ,  Julie Wilkinson ,  Ravindra Mylvaganam

    IPC分类号： G01N33/548 ,  G01N15/14 ,  G01N33/483 ,  G01N33/533 ,  G01N33/543 ,  G01N33/566 ,  G01N33/569

    CPC分类号： G01N33/533 ,  G01N33/566 ,  G01N33/56977 ,  Y10S436/80 ,  Y10S436/805

    摘要： A method for a single-measurement quantification of multiple populations of white blood cells (WBC) is based upon the labeling of different pairs of cell populations, each pair containing mutually exclusive cell receptors which are expressed at substantially similar receptor densities with labeled ligands for each receptor. One cell population is labeled with a ligand capable of binding to a first cell surface receptor which ligand is directly conjugated to a fluorescent phycobiliprotein or tandem dye; and a second cell population is labeled with a ligand capable of binding to a second cell surface receptor, which ligand is cross-linked by an aminodextran to a fluorescent phycobiliprotein or tandem dye. The phycobiliproteins upon laser excitation produce a different detectable fluorescence intensity for each cell population. Use of such pairs of conjugates enable two populations of cells with similar receptor densities to be distinguished with the use of a single color marker. Further use of additional ligands to other cell surface receptors and additional phycobiliprotein or tandem dye, or other markers in the same manner, enables the simultaneous quantification of up to 45 different cell populations with one laser line and four fluorescent colors.

    摘要翻译： 用于单次测量多种白细胞群体（WBC）的方法基于不同细胞群体对的标记，每对含有相互排斥的细胞受体，其以基本相似的受体密度表达，其中每个具有标记的配体 受体。 一个细胞群体用能够结合第一细胞表面受体的配体标记，该配体直接与荧光藻胆蛋白或串联染料缀合; 并且用能够结合第二细胞表面受体的配体标记第二细胞群，所述配体通过氨基葡聚糖与荧光藻胆蛋白或串联染料交联。 激光激发后的藻胆蛋白对每个细胞群产生不同的可检测荧光强度。 使用这样的缀合物对使得具有相似受体密度的两个细胞群可以使用单一颜色标记来区分。 以其他细胞表面受体和附加的藻胆蛋白或串联染料或其他标记物以其他方式进一步使用额外的配体使得能够用一条激光线和四种荧光颜色同时定量多达45个不同的细胞群体。