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    Method for quantification of biological material in a sample
    5.
    发明授权
    Method for quantification of biological material in a sample 有权
    用于定量样品中生物材料的方法

    公开(公告)号：US06287797B1

    公开(公告)日：2001-09-11

    申请号：US09277522

    申请日：1999-03-26

    申请人： Andrew J. Croteau ,  Mark W. Pierson ,  David E. Townsend ,  Ali Naqui

    发明人： Andrew J. Croteau ,  Mark W. Pierson ,  David E. Townsend ,  Ali Naqui

    IPC分类号： C12Q124

    CPC分类号： B01L3/5088 ,  B01L3/5085 ,  C12M23/10 ,  C12M23/12 ,  C12M23/20 ,  C12M23/38 ,  C12M41/36

    摘要： Method for detection of a biological material in a sample. The method includes the steps of liquifying the sample (if necessary) and pouring the liquified sample into the incubation plate. The incubation plate has a generally flat horizontal surface and the surface is divided into a plurality of at least 20 recessed wells. Each well is adapted to hold an aliquot of liquid and is sized and shaped, and formed of a suitable material, to hold the aliquot within the well by surface tension. Any excess liquid from the liquified sample is poured from the surface of the plate. The method then involves incubating that incubation plate until the presence or absence of the biological material is determined.

    摘要翻译： 检测样品中生物材料的方法。 该方法包括将样品液化（如果需要）并将液化样品倒入培养板中的步骤。 培养板具有大致平坦的水平表面，并且表面被分成多个至少20个凹入的孔。 每个孔适于保持液体的等分试样，并且其尺寸和形状由合适的材料形成，以通过表面张力将等分试样保持在孔内。 从液体样品的任何多余液体从板的表面倒出。 然后该方法包括孵育该培养板直到确定生物材料的存在或不存在。

    Method for quantification of biological material in a sample
    6.
    发明授权
    Method for quantification of biological material in a sample 有权
    用于定量样品中生物材料的方法

    公开(公告)号：US07122338B2

    公开(公告)日：2006-10-17

    申请号：US10349196

    申请日：2003-01-21

    申请人： Andrew J. Crouteau ,  Mark W. Pierson ,  David E. Townsend ,  Ali Naqui

    发明人： Andrew J. Crouteau ,  Mark W. Pierson ,  David E. Townsend ,  Ali Naqui

    IPC分类号： C12Q1/24

    CPC分类号： B01L3/5088 ,  B01L3/5085 ,  B01L3/50853 ,  B01L2300/0803 ,  C12M23/12 ,  C12M23/20 ,  C12M23/22 ,  C12M23/38 ,  C12M25/14 ,  C12M35/08 ,  C12M37/00 ,  C12M41/36 ,  C12Q1/24

    摘要： Methods for the detection and/or quantification of a biological material in a sample. The method includes the steps of liquefying the sample (if necessary) and pouring the liquefied sample into the incubation vessel. The incubation vessel has a generally flat horizontal surface and the surface is divided into at least one incubation site. Each incubation site is adapted to hold an aliquot of liquid and is sized and shaped, and formed of a suitable material, to hold the aliquot within the well by surface tension. Any excess liquid from the liquefied sample is poured from the surface of the incubation vessel. The method then involves incubating that incubation vessel until the presence or absence of the biological material is determined. The presence of air bubbles can be dramatically reduced by the presence of a surface acting agent in the liquid sample deposited on the device surface. Such an agent can be added to the sterile diluent used to prepare the test reagent, can be separately added to the test reagent after it is prepared, or can be added to the test sample directly while it is being prepared for testing.

    摘要翻译： 用于检测和/或定量样品中生物材料的方法。 该方法包括如下步骤：将样品液化（如有必要）并将液化的样品倒入培养容器中。 培养容器具有大致平坦的水平表面，并且将表面分成至少一个孵育位点。 每个孵化位点适于保持液体的等分试样，并且其尺寸和形状由合适的材料形成，以通过表面张力将等分试样保持在孔内。 从培养容器的表面倒出来自液化样品的任何多余的液体。 然后，该方法包括孵育该培养容器，直到确定生物材料的存在或不存在。 通过在沉积在器件表面上的液体样品中存在表面作用剂，可以显着降低气泡的存在。 可以将这样的试剂加入用于制备测试试剂的无菌稀释剂中，可以在制备试剂后分别添加到试验试剂中，或者可以在制备试剂时直接加入试验样品中。

    Self-contained incubator for growth of microorganism
    7.
    发明授权
    Self-contained incubator for growth of microorganism 失效
    用于微生物生长的独立孵化器

    公开(公告)号：US06060266A

    公开(公告)日：2000-05-09

    申请号：US43960

    申请日：1998-09-28

    申请人： Ali Naqui ,  Erwin F. Workman, Jr. ,  Haoyi Gu ,  Mark W. Pierson

    发明人： Ali Naqui ,  Erwin F. Workman, Jr. ,  Haoyi Gu ,  Mark W. Pierson

    IPC分类号： C12M1/02 ,  C12M1/12 ,  C12M1/24 ,  C12Q1/04 ,  C12Q1/02 ,  C12N1/12 ,  G01N33/53

    CPC分类号： C12M23/08 ,  C12M23/38 ,  C12M23/54 ,  C12M41/14 ,  F24J1/00 ,  Y10S435/848 ,  Y10S435/849 ,  Y10S435/975

    摘要： A self-contained incubator for growth of microorganism kit and methods for use of such a kit are provided. The kit and methods may detect the presence of microorganisms and may utilie a microorganism growth and indicator medium provided in a sample container along with a heat source, preferably generating heat through chemical means, and optionally heat shields, allowing for on-site testing of a microorganism present in a sample. The sample container may also include a removable vessel cap that includes a barrier separating the sample from a material capable of disinfecting the sample, thereby preventing contact of the sample and the material for a desired time period. The vessel cap may also be used independently in other applications.

    摘要翻译： PCT No.PCT / US96 / 15525 Sec。 371日期：1998年9月28日 102（e）1998年9月28日PCT PCT 1996年9月27日PCT公布。 第WO97 / 12029号公报 日期1997年4月3日提供了用于生长微生物试剂盒的独立培养箱和使用这种试剂盒的方法。 试剂盒和方法可以检测微生物的存在，并且可以利用提供在样品容器中的微生物生长和指示剂介质以及热源，优选通过化学方法产生热量，并且可选地加热屏蔽，允许现场测试 存在于样品中的微生物。 样品容器还可以包括可移除的容器盖，其包括将样品与能够对样品进行消毒的材料隔离的屏障，从而防止样品和材料在期望的时间段内接触。 容器盖也可以在其它应用中独立使用。

    Method for quantification of biological material in a sample
    9.
    发明授权
    Method for quantification of biological material in a sample 失效
    用于定量样品中生物材料的方法

    公开(公告)号：US5700655A

    公开(公告)日：1997-12-23

    申请号：US606229

    申请日：1996-02-23

    申请人： Andrew J. Croteau ,  Mark W. Pierson ,  David E. Townsend ,  Ali Naqui

    发明人： Andrew J. Croteau ,  Mark W. Pierson ,  David E. Townsend ,  Ali Naqui

    IPC分类号： B01L3/00 ,  C12M1/20 ,  C12Q1/24 ,  C12M1/22

    CPC分类号： B01L3/5088 ,  B01L3/5085 ,  C12M23/10 ,  C12M23/12 ,  C12M23/20 ,  C12M23/38 ,  C12M41/36 ,  C12M37/00

    摘要： Method for detection of a biological material in a sample. The method includes the steps of liquifying the sample (if necessary) and pouring the liquified sample into the incubation plate. The incubation plate has a generally flat horizontal surface and the surface is divided into a plurality of at least 20 recessed wells. Each well is adapted to hold an aliquot of liquid and is sized and shaped, and formed of a suitable material, to hold the aliquot within the well by surface tension. Any excess liquid from the liquified sample is poured from the surface of the plate. The method then involves incubating that incubation plate until the presence or absence of the biological material is determined.

    摘要翻译： 检测样品中生物材料的方法。 该方法包括将样品液化（如果需要）并将液化样品倒入培养板中的步骤。 培养板具有大致平坦的水平表面，并且表面被分成多个至少20个凹入的孔。 每个孔适于保持液体的等分试样，并且其尺寸和形状由合适的材料形成，以通过表面张力将等分试样保持在孔内。 从液体样品的任何多余液体从板的表面倒出。 然后该方法包括孵育该培养板直到确定生物材料的存在或不存在。

    Threaded temperature indicating plug for hot oil housings such as transmissions and the like
    10.
    发明授权
    Threaded temperature indicating plug for hot oil housings such as transmissions and the like 失效
    螺纹温度指示插头用于热油外壳如变速器等

    公开(公告)号：US5076708A

    公开(公告)日：1991-12-31

    申请号：US523861

    申请日：1990-05-14

    申请人： Mark W. Pierson

    发明人： Mark W. Pierson

    IPC分类号： F16H57/04 ,  F16H59/72 ,  G01K11/12

    CPC分类号： F16H57/0413 ,  G01K11/12 ,  F16H59/72

    摘要： A threaded temperature indicating plug for hot oil containing housings such as vehicle transmissions and the like that can replace the standard oil level plugs of the same and as well afford a plug that from which the temperature at which the oil may have overheated will be recorded and whether there is a definite need to change the oil in the transmission. Liquid crystal areas are provided on an indicator label that is fixed to the plug bolt shank in a liquid-tight manner by a fixed covering and for direct contact with the oil body or a splash from oil at a lowered level.

    摘要翻译： 将记录用于热油容纳壳体（例如车辆变速器等）的带螺纹的温度指示塞，其可以替代其中的标准油位塞，并且还可以记录油可能过热的温度的塞子， 是否有明确的需要改变传输中的油。 液晶区域设置在指示标签上，该标签通过固定的盖子以液密的方式固定到插头螺栓柄上，并且与油体直接接触或者在较低的水平处从油中溅出。