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    Viral vectors for use in monitoring HIV drug resistance
    3.
    发明授权
    Viral vectors for use in monitoring HIV drug resistance 失效
    用于监测HIV耐药性的病毒载体

    公开(公告)号：US06410013B1

    公开(公告)日：2002-06-25

    申请号：US09559244

    申请日：2000-04-26

    申请人： Jian-yun Dong

    发明人： Jian-yun Dong

    IPC分类号： A01N6300

    CPC分类号： C12Q1/6883 ,  C12N2503/00 ,  C12N2799/022 ,  C12N2799/027 ,  C12Q1/6897 ,  C12Q1/703 ,  G01N33/56988

    摘要： Recombinant expression vectors and methods are provided for detecting HIV and monitoring HIV drug resistance. The method comprises: taking a culture of cells; adding a recombinant viral vector into the culture to transduce the cells, the recombinant viral vector comprising a reporter sequence comprising a reporter gene whose expression is regulated by a protein specific to HIV viruses which is expressed from a genome of an HIV virus upon infection of a cell in the culture that is transduced by the recombinant viral vector, and a receptor sequence comprising CD 4 and one or more coreceptor genes, expression of the coreceptor genes facilitating productive infection of the transduced cell and enabling HIV virus which has infected the transduced cell to replicate and infect non-infected cells in the culture of the cells transduced by the recombinant viral vector; infecting the transduced cells with a sample containing HIV; adding one or more anti-HIV agents to the cell culture; and detecting a change in a level of expression of the reporter gene in cells.

    摘要翻译： 提供重组表达载体和方法用于检测HIV并监测HIV耐药性。 该方法包括：取细胞培养; 将重组病毒载体加入到培养物中以转导细胞，所述重组病毒载体包含包含报告基因的报告基因序列，其报告基因的表达受HIV病毒特异性蛋白调节，所述蛋白质在感染HIV病毒时从HIV病毒的基因组表达 由重组病毒载体转导的培养物中的细胞，以及包含CD 4和一种或多种共受体基因的受体序列，促进转导细胞的生产性感染的共同受体基因的表达，以及使感染转导的细胞的HIV病毒 在由重组病毒载体转导的细胞的培养物中复制和感染未感染的细胞; 用含有HIV的样品感染转导的细胞; 向细胞培养物中加入一种或多种抗HIV剂; 并检测细胞中报道基因表达水平的变化。

    Method for producing recombinant cells for detecting HIV
    4.
    发明授权
    Method for producing recombinant cells for detecting HIV 失效
    用于产生用于检测HIV的重组细胞的方法

    公开(公告)号：US06967076B2

    公开(公告)日：2005-11-22

    申请号：US10112579

    申请日：2002-03-29

    申请人： Jian-yun Dong

    发明人： Jian-yun Dong

    IPC分类号： C12N15/09 ,  A01N63/00 ,  A61K39/12 ,  A61K39/23 ,  A61K39/235 ,  A61K48/00 ,  C07H21/04 ,  C12N5/00 ,  C12N5/02 ,  C12N5/06 ,  C12N5/10 ,  C12N5/16 ,  C12N13/00 ,  C12N15/00 ,  C12N15/12 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/85 ,  C12N15/87 ,  C12P21/06 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/70 ,  C12R1/93 ,  G01N33/15 ,  G01N33/50 ,  G01N33/569

    CPC分类号： C12Q1/6883 ,  C12N2503/00 ,  C12N2799/022 ,  C12N2799/027 ,  C12Q1/6897 ,  C12Q1/703 ,  G01N33/56988

    摘要： Recombinant expression vectors and methods are provided for detecting HIV and monitoring HIV drug resistance. The method comprises: taking a culture of cells; adding a recombinant viral vector into the culture to transduce the cells, the recombinant viral vector comprising a reporter sequence comprising a reporter gene whose expression is regulated by a protein specific to HIV viruses which is expressed from a genome of an HIV virus upon infection of a cell in the culture that is transduced by the recombinant viral vector, and a receptor sequence comprising CD 4 and one or more coreceptor genes, expression of the coreceptor genes facilitating productive infection of the transduced cell and enabling HIV virus which has infected the transduced cell to replicate and infect non-infected cells in the culture of the cells transduced by the recombinant viral vector; infecting the transduced cells with a sample containing HIV; adding one or more anti-HIV agents to the cell culture; and detecting a change in a level of expression of the reporter gene in cells.

    摘要翻译： 提供重组表达载体和方法用于检测HIV并监测HIV耐药性。 该方法包括：取细胞培养; 将重组病毒载体加入到培养物中以转导细胞，所述重组病毒载体包含包含报告基因的报告基因序列，其报告基因的表达受HIV病毒特异性蛋白调节，所述蛋白质在感染HIV病毒时从HIV病毒的基因组表达 由重组病毒载体转导的培养物中的细胞，以及包含CD 4和一种或多种共受体基因的受体序列，促进转导细胞的生产性感染的共同受体基因的表达，以及使感染转导的细胞的HIV病毒 在由重组病毒载体转导的细胞的培养物中复制和感染未感染的细胞; 用含有HIV的样品感染转导的细胞; 向细胞培养物中加入一种或多种抗HIV剂; 并检测细胞中报道基因表达水平的变化。