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公开(公告)号：US09115346B2
公开(公告)日：2015-08-25
申请号：US12826960
申请日：2010-06-30
CPC分类号： C12N9/20 , C12Y301/01003
摘要： The inventors have developed a method using protein engineering to produce lipolytic enzymes having a relatively high activity for one ester bond in an amphiphilic substrate with two lipophilic groups) and a relatively low activity for the ester bond in an amphiphilic substrate with one lipophilic group, e.g. a relatively high phospholipase activity and a relatively low lysophospholipase activity.
摘要翻译： 本发明人开发了一种使用蛋白质工程的方法来生产对于具有两个亲脂基团的两亲底物中的一个酯键具有相对较高活性的脂肪分解酶），并且在具有一个亲油基团的两亲底物中具有较低的酯键活性。 相对较高的磷脂酶活性和较低的溶血磷脂酶活性。
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公开(公告)号：US20100047388A1
公开(公告)日：2010-02-25
申请号：US12612398
申请日：2009-11-04
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译： 本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。 更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。 多肽可以特别地对极性脂质具有活性。
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公开(公告)号：US20080311242A9
公开(公告)日：2008-12-18
申请号：US10556511
申请日：2004-04-29
申请人： Kim Borch , Luise Erlandsen , Jesper Vind , Allan Svendsen , Christel Jorgensen
发明人： Kim Borch , Luise Erlandsen , Jesper Vind , Allan Svendsen , Christel Jorgensen
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译： 本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。 更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。 多肽可以特别地对极性脂质具有活性。
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公开(公告)号：US08298800B2
公开(公告)日：2012-10-30
申请号：US12612398
申请日：2009-11-04
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译： 本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。 更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。 多肽可以特别地对极性脂质具有活性。
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公开(公告)号：US07465570B2
公开(公告)日：2008-12-16
申请号：US10556511
申请日：2004-04-29
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译： 本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。 更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。 多肽可以特别地对极性脂质具有活性。
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公开(公告)号：US20080293120A1
公开(公告)日：2008-11-27
申请号：US12169679
申请日：2008-07-09
IPC分类号： C12N9/20
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译： 本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。 更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。 多肽可以特别地对极性脂质具有活性。
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公开(公告)号：US07638317B2
公开(公告)日：2009-12-29
申请号：US12169679
申请日：2008-07-09
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译： 本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。 更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。 多肽可以特别地对极性脂质具有活性。
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公开(公告)号：US20060228446A1
公开(公告)日：2006-10-12
申请号：US10556511
申请日：2004-04-29
申请人： Kim Borch , Luise Erlandsen , Jesper Vind , Allan Svendsen , Christel Jorgensen
发明人： Kim Borch , Luise Erlandsen , Jesper Vind , Allan Svendsen , Christel Jorgensen
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译： 本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。 更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。 多肽可以特别地对极性脂质具有活性。
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公开(公告)号：US20050255544A1
公开(公告)日：2005-11-17
申请号：US10495597
申请日：2003-01-16
CPC分类号： C12N9/20 , C12Y301/01003
摘要： The inventors have developed a method using protein engineering to produce lipolytic enzymes having a relatively high activity for one ester bond in an amphiphilic substrate with two lipophilic groups) and a relatively low activity for the ester bond in an amphiphilic substrate with one lipophilic group, e.g. a relatively high phospholipase activity and a relatively low lysophospholipase activity.
摘要翻译： 本发明人开发了一种使用蛋白质工程的方法来生产对于具有两个亲脂基团的两亲底物中的一个酯键具有相对较高活性的脂肪分解酶），并且在具有一个亲油基团的两亲底物中具有较低的酯键活性。 相对较高的磷脂酶活性和较低的溶血磷脂酶活性。
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公开(公告)号：US20120034338A1
公开(公告)日：2012-02-09
申请号：US13259807
申请日：2010-04-20
申请人： Anne Mette Bhatia Frederiksen , Jesper Vind , Lars Saaby Pedersen , Hans Peter Heldt-Hansen , Marco Malten
发明人： Anne Mette Bhatia Frederiksen , Jesper Vind , Lars Saaby Pedersen , Hans Peter Heldt-Hansen , Marco Malten
IPC分类号： A23L2/70 , A23C9/12 , A23L2/02 , C12G1/00 , C12C7/175 , C12C7/14 , A23L2/72 , A23L2/80 , C12C7/16 , C12C11/00 , C12C7/00
CPC分类号： A23L2/84 , A23C9/1203 , A23L29/06 , C12C5/004 , C12C7/28 , C12H1/0408
摘要： A method of separating an enzyme construct from a solution, comprising providing an enzyme construct comprising an enzyme fused to a silaffin; adding the enzyme construct to a solution; adding a silica to the solution and separating the silica bound enzyme construct from the solution, wherein the solution is a beverage or a beverage intermediate.
摘要翻译： 一种从溶液中分离酶构建体的方法，包括提供包含与石蜡融合的酶的酶构建体; 将酶构建体加入溶液中; 向溶液中加入二氧化硅，并将二氧化硅结合的酶构建体与溶液分离，其中溶液是饮料或饮料中间体。
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