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公开(公告)号：US20100183766A1
公开(公告)日：2010-07-22
申请号：US12729877
申请日：2010-03-23
CPC分类号： C12N9/1074 , C12N9/2408 , C12N9/2414 , C12N9/2417
摘要： The inventors have developed a method of modifying the amino acid sequence of a CGTase to obtain variants. The variants may form linear oligosaccharides as an initial product by starch hydrolysis and a reduced amount of cyclodextrin and may be useful for anti-staling in baked products. The method is based on a comparison of three-dimensional (3D) structures of the CGTase with the structure of a maltogenic alpha-amylase where one or both models includes a substrate. The invention also provides novel CGTase variants.
摘要翻译： 发明人已经开发出修饰CGTase的氨基酸序列以获得变体的方法。 变体可以通过淀粉水解形成线性寡糖作为初始产物，并减少量的环糊精，并且可用于烘焙产品中的抗老化。 该方法基于CGTase的三维（3D）结构与麦芽糖α-淀粉酶的结构的比较，其中一个或两个模型包括底物。 本发明还提供了新的CGTase变体。
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公开(公告)号：US07892806B2
公开(公告)日：2011-02-22
申请号：US12698524
申请日：2010-02-02
IPC分类号： C12N9/30 , C12N9/28 , C12N9/26 , C12N9/24 , C12N9/00 , C12P19/14 , C12P19/16 , C12P19/20 , C07H21/04 , A21D2/00
CPC分类号： C12N9/2417 , A21D2/267 , A21D8/042 , C12N9/242
摘要： The inventors have developed a method of altering the amino acid sequence of a fungal alpha-amylase to obtain variants, and they have used the method to construct such variants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The invention also provides novel fungal alpha-amylase variants.
摘要翻译： 发明人已经开发了改变真菌α-淀粉酶的氨基酸序列以获得变体的方法，并且已经使用该方法来构建这些变体。 这些变体可用于烘焙产品中的防老化。 因此，本发明提供了基于真菌α-淀粉酶和产麦芽糖α-淀粉酶的三维（3D）结构的比较来构建真菌α-淀粉酶变体的方法。 一种或两种型号包括基材。 本发明还提供了新的真菌α-淀粉酶变体。
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公开(公告)号：US20100136655A1
公开(公告)日：2010-06-03
申请号：US12698524
申请日：2010-02-02
IPC分类号： C12N9/30
CPC分类号： C12N9/2417 , A21D2/267 , A21D8/042 , C12N9/242
摘要： The inventors have developed a method of altering the amino acid sequence of a fungal alpha-amylase to obtain variants, and they have used the method to construct such variants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The invention also provides novel fungal alpha-amylase variants.
摘要翻译： 发明人已经开发了改变真菌α-淀粉酶的氨基酸序列以获得变体的方法，并且已经使用该方法来构建这些变体。 这些变体可用于烘焙产品中的防老化。 因此，本发明提供了基于真菌α-淀粉酶和产麦芽糖α-淀粉酶的三维（3D）结构的比较来构建真菌α-淀粉酶变体的方法。 一种或两种型号包括基材。 本发明还提供了新的真菌α-淀粉酶变体。
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公开(公告)号：US20070148287A1
公开(公告)日：2007-06-28
申请号：US10562021
申请日：2004-07-01
申请人： Allan Svendsen , Lars Beier , Tina Spendler , Morten Jensen , Christel Jorgensen
发明人： Allan Svendsen , Lars Beier , Tina Spendler , Morten Jensen , Christel Jorgensen
CPC分类号： C12N9/1074 , C12N9/2408 , C12N9/2414 , C12N9/2417
摘要： The inventors have developed a method of modifying the amino add sequence of a CGTase to obtain variants. The variants may form linear oligosaccharides as an initial product by starch hydrolysis and a reduced amount of cyclodextrin and may be useful for anti-staling in baked products. The method is based on a comparison of three-dimensional (3D) structures of the CGTase with the structure of a maltogenic alpha-amylase where one or both models includes a substrate. The invention also provides novel CGTase variants.
摘要翻译： 本发明人已经开发了一种修饰CGTase的氨基酸序列以获得变体的方法。 变体可以通过淀粉水解形成线性寡糖作为初始产物，并减少量的环糊精，并且可用于烘焙产品中的抗老化。 该方法基于CGTase的三维（3D）结构与麦芽糖α-淀粉酶的结构的比较，其中一个或两个模型包括底物。 本发明还提供了新的CGTase变体。
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公开(公告)号：US20070264700A1
公开(公告)日：2007-11-15
申请号：US10536101
申请日：2004-08-23
申请人： Allan Svendsen , Lars Beier , Jesper Vind , Tina Spendler , Morten Jensen
发明人： Allan Svendsen , Lars Beier , Jesper Vind , Tina Spendler , Morten Jensen
IPC分类号： C12N9/30
CPC分类号： C12N9/2417 , A21D2/267 , A21D8/042 , C12N9/242
摘要： The inventors have developed a method of altering the amino acid sequence of a fungal alpha-amylase to obtain variants, and they have used the method to construct such variants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The invention also provides novel fungal alpha-amylase variants.
摘要翻译： 发明人已经开发了改变真菌α-淀粉酶的氨基酸序列以获得变体的方法，并且已经使用该方法来构建这些变体。 这些变体可用于烘焙产品中的防老化。 因此，本发明提供了基于真菌α-淀粉酶和产麦芽糖α-淀粉酶的三维（3D）结构的比较来构建真菌α-淀粉酶变体的方法。 一种或两种型号包括基材。 本发明还提供了新的真菌α-淀粉酶变体。
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公开(公告)号：US20110076741A1
公开(公告)日：2011-03-31
申请号：US12952695
申请日：2010-11-23
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。
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公开(公告)号：US07306936B2
公开(公告)日：2007-12-11
申请号：US10146327
申请日：2002-05-15
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。
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公开(公告)号：US08298800B2
公开(公告)日：2012-10-30
申请号：US12612398
申请日：2009-11-04
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译： 本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。 更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。 多肽可以特别地对极性脂质具有活性。
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公开(公告)号：US08124395B2
公开(公告)日：2012-02-28
申请号：US12952695
申请日：2010-11-23
申请人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
发明人： Carsten Andersen , Christel Thea Jorgensen , Henrik Bisgard-Frantzen , Allan Svendsen , Soren Kjaerulff
CPC分类号： C12N9/2417 , C07K2319/00 , C12P19/04 , C12P19/14 , C12Y302/01001 , Y02E50/17 , Y02P20/52
摘要： The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.
摘要翻译： 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体表现出改变的性质，特别是降低了切割接近分支点的底物的能力，以及相对于母体改善的底物特异性和/或改善的比活性 α-淀粉酶。
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公开(公告)号：US07465570B2
公开(公告)日：2008-12-16
申请号：US10556511
申请日：2004-04-29
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12Y301/01032
摘要： The inventors have developed improved polypeptides by substituting or deleting specified amino acids in fungal lipolytic enzymes. More particularly, the polypeptides result in a reduction of dough stickiness when they are added to a dough. The polypeptides may particularly have activity on polar lipids.
摘要翻译： 本发明人通过在真菌脂肪分解酶中取代或缺失特定的氨基酸而开发了改进的多肽。 更具体地说，当将多肽加入面团时，多肽导致面团粘性降低。 多肽可以特别地对极性脂质具有活性。
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