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公开(公告)号：US07892806B2
公开(公告)日：2011-02-22
申请号：US12698524
申请日：2010-02-02
IPC分类号： C12N9/30 , C12N9/28 , C12N9/26 , C12N9/24 , C12N9/00 , C12P19/14 , C12P19/16 , C12P19/20 , C07H21/04 , A21D2/00
CPC分类号： C12N9/2417 , A21D2/267 , A21D8/042 , C12N9/242
摘要： The inventors have developed a method of altering the amino acid sequence of a fungal alpha-amylase to obtain variants, and they have used the method to construct such variants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The invention also provides novel fungal alpha-amylase variants.
摘要翻译： 发明人已经开发了改变真菌α-淀粉酶的氨基酸序列以获得变体的方法，并且已经使用该方法来构建这些变体。 这些变体可用于烘焙产品中的防老化。 因此，本发明提供了基于真菌α-淀粉酶和产麦芽糖α-淀粉酶的三维（3D）结构的比较来构建真菌α-淀粉酶变体的方法。 一种或两种型号包括基材。 本发明还提供了新的真菌α-淀粉酶变体。
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公开(公告)号：US20100136655A1
公开(公告)日：2010-06-03
申请号：US12698524
申请日：2010-02-02
IPC分类号： C12N9/30
CPC分类号： C12N9/2417 , A21D2/267 , A21D8/042 , C12N9/242
摘要： The inventors have developed a method of altering the amino acid sequence of a fungal alpha-amylase to obtain variants, and they have used the method to construct such variants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The invention also provides novel fungal alpha-amylase variants.
摘要翻译： 发明人已经开发了改变真菌α-淀粉酶的氨基酸序列以获得变体的方法，并且已经使用该方法来构建这些变体。 这些变体可用于烘焙产品中的防老化。 因此，本发明提供了基于真菌α-淀粉酶和产麦芽糖α-淀粉酶的三维（3D）结构的比较来构建真菌α-淀粉酶变体的方法。 一种或两种型号包括基材。 本发明还提供了新的真菌α-淀粉酶变体。
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公开(公告)号：US20100183766A1
公开(公告)日：2010-07-22
申请号：US12729877
申请日：2010-03-23
CPC分类号： C12N9/1074 , C12N9/2408 , C12N9/2414 , C12N9/2417
摘要： The inventors have developed a method of modifying the amino acid sequence of a CGTase to obtain variants. The variants may form linear oligosaccharides as an initial product by starch hydrolysis and a reduced amount of cyclodextrin and may be useful for anti-staling in baked products. The method is based on a comparison of three-dimensional (3D) structures of the CGTase with the structure of a maltogenic alpha-amylase where one or both models includes a substrate. The invention also provides novel CGTase variants.
摘要翻译： 发明人已经开发出修饰CGTase的氨基酸序列以获得变体的方法。 变体可以通过淀粉水解形成线性寡糖作为初始产物，并减少量的环糊精，并且可用于烘焙产品中的抗老化。 该方法基于CGTase的三维（3D）结构与麦芽糖α-淀粉酶的结构的比较，其中一个或两个模型包括底物。 本发明还提供了新的CGTase变体。
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公开(公告)号：US20070264700A1
公开(公告)日：2007-11-15
申请号：US10536101
申请日：2004-08-23
申请人： Allan Svendsen , Lars Beier , Jesper Vind , Tina Spendler , Morten Jensen
发明人： Allan Svendsen , Lars Beier , Jesper Vind , Tina Spendler , Morten Jensen
IPC分类号： C12N9/30
CPC分类号： C12N9/2417 , A21D2/267 , A21D8/042 , C12N9/242
摘要： The inventors have developed a method of altering the amino acid sequence of a fungal alpha-amylase to obtain variants, and they have used the method to construct such variants. The variants may be useful for anti-staling in baked products. Accordingly, the invention provides a method of constructing fungal alpha-amylase variants based on a comparison of three-dimensional (3D) structures of the fungal alpha-amylase and a maltogenic alpha-amylase. One or both models includes a substrate. The invention also provides novel fungal alpha-amylase variants.
摘要翻译： 发明人已经开发了改变真菌α-淀粉酶的氨基酸序列以获得变体的方法，并且已经使用该方法来构建这些变体。 这些变体可用于烘焙产品中的防老化。 因此，本发明提供了基于真菌α-淀粉酶和产麦芽糖α-淀粉酶的三维（3D）结构的比较来构建真菌α-淀粉酶变体的方法。 一种或两种型号包括基材。 本发明还提供了新的真菌α-淀粉酶变体。
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公开(公告)号：US20070148287A1
公开(公告)日：2007-06-28
申请号：US10562021
申请日：2004-07-01
申请人： Allan Svendsen , Lars Beier , Tina Spendler , Morten Jensen , Christel Jorgensen
发明人： Allan Svendsen , Lars Beier , Tina Spendler , Morten Jensen , Christel Jorgensen
CPC分类号： C12N9/1074 , C12N9/2408 , C12N9/2414 , C12N9/2417
摘要： The inventors have developed a method of modifying the amino add sequence of a CGTase to obtain variants. The variants may form linear oligosaccharides as an initial product by starch hydrolysis and a reduced amount of cyclodextrin and may be useful for anti-staling in baked products. The method is based on a comparison of three-dimensional (3D) structures of the CGTase with the structure of a maltogenic alpha-amylase where one or both models includes a substrate. The invention also provides novel CGTase variants.
摘要翻译： 本发明人已经开发了一种修饰CGTase的氨基酸序列以获得变体的方法。 变体可以通过淀粉水解形成线性寡糖作为初始产物，并减少量的环糊精，并且可用于烘焙产品中的抗老化。 该方法基于CGTase的三维（3D）结构与麦芽糖α-淀粉酶的结构的比较，其中一个或两个模型包括底物。 本发明还提供了新的CGTase变体。
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公开(公告)号：US20120083025A1
公开(公告)日：2012-04-05
申请号：US13289634
申请日：2011-11-04
IPC分类号： C12N9/96
摘要： The present invention relates to hybrid polypeptides having a first amino acid sequence having endo-amylase activity and a second amino acid sequence having carbohydrate binding activity. The present invention also relates to the use of the hybrid polypeptides in starch processing and baking.
摘要翻译： 本发明涉及具有内切淀粉酶活性的第一氨基酸序列和具有碳水化合物结合活性的第二个氨基酸序列的杂合多肽。 本发明还涉及杂交多肽在淀粉加工和烘烤中的用途。
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公开(公告)号：US08440444B2
公开(公告)日：2013-05-14
申请号：US13289634
申请日：2011-11-04
摘要： The present invention relates to hybrid polypeptides having a first amino acid sequence having endo-amylase activity and a second amino acid sequence having carbohydrate binding activity. The present invention also relates to the use of the hybrid polypeptides in starch processing and baking.
摘要翻译： 本发明涉及具有内切淀粉酶活性的第一氨基酸序列和具有碳水化合物结合活性的第二个氨基酸序列的杂合多肽。 本发明还涉及杂交多肽在淀粉加工和烘烤中的用途。
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公开(公告)号：US20060147581A1
公开(公告)日：2006-07-06
申请号：US11315801
申请日：2005-12-22
摘要： The present invention relates to a polypeptide and the use thereof.
摘要翻译： 本发明涉及多肽及其用途。
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公开(公告)号：US08679774B2
公开(公告)日：2014-03-25
申请号：US12849911
申请日：2010-08-04
申请人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
发明人： Kirsten Bojsen , Allan Svendsen , Claus Crone Fuglsang , Shamkant Anant Patkar , Kim Borch , Jesper Vind , Andreas Petri , Sanne O. Schroder Glad , Gitte Budolfsen
CPC分类号： C12Y301/01003 , A21D8/042 , C11B3/003 , C11D3/38627 , C12N9/20 , C12P9/00
摘要： The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.
摘要翻译： 可以通过改变脂肪分解酶的限定区域中的氨基酸序列来修饰脂解酶的底物特异性，从而增加所需活性的水平或降低不需要的活性的水平。 因此，本发明人已经开发了具有修饰的氨基酸序列的脂肪分解酶变体，其具有底物特异性，其可以针对特定用途进行定制。
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公开(公告)号：US08101392B2
公开(公告)日：2012-01-24
申请号：US12905348
申请日：2010-10-15
申请人： Bjarne Roenfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
发明人： Bjarne Roenfeldt Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Vang Hendriksen , Torben Peter Frandsen
IPC分类号： C12N9/34 , C12N9/30 , C12N9/26 , C12N9/24 , C12N9/00 , C12P19/20 , C12P19/14 , C07H21/04 , C07K1/00
CPC分类号： C12N9/2428 , C12P19/14 , C12P19/20 , Y02E50/17
摘要： The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.
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