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    Monolayer and electrode for detecting a label-bearing target and method of use thereof
    1.
    发明授权
    Monolayer and electrode for detecting a label-bearing target and method of use thereof 有权
    用于检测标记物的单层和电极及其使用方法

    公开(公告)号：US06387625B1

    公开(公告)日：2002-05-14

    申请号：US09596607

    申请日：2000-06-19

    申请人： Allen E. Eckhardt ,  Jill C. Mikulecky ,  Mary E. Napier ,  Robert S. Thomas ,  H. Holden Thorp

    发明人： Allen E. Eckhardt ,  Jill C. Mikulecky ,  Mary E. Napier ,  Robert S. Thomas ,  H. Holden Thorp

    IPC分类号： C12Q168

    CPC分类号： B82Y30/00 ,  B82Y15/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  Y10S435/81 ,  Y10T436/143333 ,  C12Q2525/197 ,  C12Q2563/113

    摘要： An electrode for detecting interactions between members of a binding pair, which electrode has been modified by formation of a non-conductive self-assembled monolayer, and a method of detecting biomolecules, such as nucleic acids or other targets, including receptors, ligands, antigens or antibodies, utilizing such an electrode. When contacted with a target nucleic acid, an oligonucleotide probe coupled to the self-assembled monolayer reacts with the target nucleic acid form a hybridized nucleic acid on the modified electrode surface. The hybridized nucleic acid is reacted with a transition metal complex capable of oxidizing a preselected base in the hybridized nucleic acid in an oxidation-reduction reaction, the oxidation-reduction reaction is detected, and the presence or absence of the nucleic acid is determined from the detected oxidation-reduction reaction.

    摘要翻译： 用于检测结合对的成员之间的相互作用的电极，该电极已经通过形成非导电自组装单层而被修饰，以及检测生物分子例如核酸或其它靶，包括受体，配体，抗原的方法 或抗体，利用这种电极。 当与目标核酸接触时，与自组装单层结合的寡核苷酸探针与目标核酸反应，形成经修饰的电极表面的杂交核酸。 杂交的核酸与氧化还原反应中能够氧化杂交核酸中的预选碱基的过渡金属络合物反应，检测氧化还原反应，核酸的存在或不存在从 检测到氧化还原反应。

    Monolayer and electrode for detecting a label-bearing target and method of use thereof
    2.
    发明授权
    Monolayer and electrode for detecting a label-bearing target and method of use thereof 有权
    用于检测标记物的单层和电极及其使用方法

    公开(公告)号：US6127127A

    公开(公告)日：2000-10-03

    申请号：US296929

    申请日：1999-04-22

    申请人： Allen E. Eckhardt ,  Jill C. Mikulecky ,  Mary E. Napier ,  Robert S. Thomas ,  H. Holden Thorp

    发明人： Allen E. Eckhardt ,  Jill C. Mikulecky ,  Mary E. Napier ,  Robert S. Thomas ,  H. Holden Thorp

    IPC分类号： G01N33/483 ,  C07F9/22 ,  C07K1/113 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  G01N33/547 ,  G01N33/553 ,  G01N33/566

    CPC分类号： B82Y30/00 ,  B82Y15/00 ,  C12Q1/6825

    摘要： An electrode for detecting interactions between members of a binding pair, which electrode has been modified by formation of a non-conductive self-assembled monolayer, and a method of detecting biomolecules, such as nucleic acids or other targets, including receptors, ligands, antigens or antibodies, utilizing such an electrode. When contacted with a target nucleic acid, an oligonucleotide probe coupled to the self-assembled monolayer reacts with the target nucleic acid to form a hybridized nucleic acid on the modified electrode surface. The hybridized nucleic acid is reacted with a transition metal complex capable of oxidizing a preselected base in the hybridized nucleic acid in an oxidation-reduction reaction, the oxidation-reduction reaction is detected, and the presence or absence of the nucleic acid is determined from the detected oxidation-reduction reaction.

    摘要翻译： 用于检测结合对的成员之间的相互作用的电极，该电极已经通过形成非导电自组装单层而被修饰，以及检测生物分子例如核酸或其它靶，包括受体，配体，抗原的方法 或抗体，利用这种电极。 当与靶核酸接触时，与自组装单层结合的寡核苷酸探针与靶核酸反应，以在修饰的电极表面上形成杂交的核酸。 杂交的核酸与氧化还原反应中能够氧化杂交核酸中的预选碱基的过渡金属络合物反应，检测氧化还原反应，核酸的存在或不存在从 检测到氧化还原反应。

    Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof
    7.
    发明授权
    Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof 失效
    用于检测核酸杂交的聚合物电极及其使用方法

    公开(公告)号：US5968745A

    公开(公告)日：1999-10-19

    申请号：US950503

    申请日：1997-10-14

    申请人： H. Holden Thorp ,  Carson R. Loomis ,  Mary E. Napier

    发明人： H. Holden Thorp ,  Carson R. Loomis ,  Mary E. Napier

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： B82Y15/00 ,  B82Y30/00 ,  C12Q1/6816 ,  C12Q1/6825

    摘要： A polymer-electrode including (a) a substrate having a conductive working surface; and (b) a polymer layer on the conductive working surface. The polymer layer has a plurality of microfluidic reaction openings distributed throughout the layer. An oligonucleotide probe can be attached to the polymer layer and is available to capture target nucleic acid. A soluble mediator can diffuse freely and transfer electrons from the preselected base in the hybridized nucleic acid to the conductive working surface of the substrate. An electronic signal generated from the electron transfer reaction is detected and quantitated.

    摘要翻译： 1.一种聚合物电极，包括（a）具有导电性工作面的基板; 和（b）导电工作表面上的聚合物层。 聚合物层具有分布在整个层中的多个微流体反应开口。 寡核苷酸探针可以连接到聚合物层并且可用于捕获靶核酸。 可溶性介体可以自由扩散，并将电子从杂交核酸中的预选碱转移到基底的导电工作表面。 从电子转移反应产生的电子信号被检测和定量。