公开(公告)号：US11920153B2

公开(公告)日：2024-03-05

申请号：US16339164

申请日：2017-10-03

申请人： Alma Mater Studiorum—Università di Bologna

发明人： Stefania Rapino ,  Francesco Zerbetto ,  Gastone Castellani ,  Stefano Salvioli ,  Beatrice Fraboni ,  Isabella Zironi ,  Maria Conte ,  Claudio Franceschi ,  Daniela Salvatore ,  Maila Becconi

IPC分类号： C12N5/00 ,  C12N5/09 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/08 ,  C12N11/04 ,  C12N11/06 ,  C12N11/087 ,  C12N11/089 ,  C12N11/12 ,  G01N33/50

CPC分类号： C12N5/0018 ,  C12N5/0068 ,  C12N5/0693 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0036 ,  C12N9/0053 ,  C12N9/0061 ,  C12N9/0065 ,  C12N9/0069 ,  C12N9/0093 ,  C12N11/04 ,  C12N11/06 ,  C12N11/087 ,  C12N11/089 ,  C12N11/12 ,  C12Y101/03004 ,  C12Y106/03001 ,  C12Y109/03001 ,  C12Y110/03002 ,  C12Y111/01006 ,  C12Y113/12004 ,  C12Y117/03002 ,  G01N33/5008 ,  C12N2500/02 ,  C12N2501/71 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/74 ,  C12N2533/76 ,  C12N2533/78 ,  C12N2533/80 ,  C12N2537/10

摘要： The present invention relates to compositions comprising a polymeric matrix or a gel containing functional enzymes capable of re-creating under culture conditions the cell microenvironment existing in vivo. The present invention also relates to devices for cell cultures comprising such compositions, in particular hydrogel and the use thereof to control the chemical microenvironment of a cell culture or mimic physiological or pathological conditions of the in vivo cells. The compositions and the devices described herein could be also used in vitro for evaluating the therapeutic effect of a compound on a determined cell line or on primary cells.