-
公开(公告)号：US20240093229A1
公开(公告)日：2024-03-21
申请号：US18325321
申请日：2023-05-30
CPC分类号： C12N15/85 , A61K48/005 , A61K48/0058 , A61K48/0066 , C07K14/47 , C12N9/0053 , C12N9/0089 , C12N9/1085 , C12N15/8509 , C12Y109/03001 , C07K2319/07 , C12N2800/22 , C12N2810/80
摘要： An expression vector containing appropriate mitochondrion-targeting sequences (MTS) and appropriate 3′UTR sequences provides efficient and stable delivery of a mRNA encoding a protein (CDS) to the mitochondrion of a mammalian cell. The MTS and 3′UTR sequences guide the CDS mRNA from the nuclear compartment of the cell to mitochondrion-bound polysomes, where the CDS is translated. This provides an efficient translocation of a mature functional protein into the mitochondria. A method of targeting mRNA expressed in the nuclear compartment of a mammalian cell to the mitochondrion is also provided. The vector and methods can be used to treat defects in mitochondrial function.
-
公开(公告)号：US11702671B2
公开(公告)日：2023-07-18
申请号：US17331097
申请日：2021-05-26
CPC分类号： C12N15/85 , A61K48/005 , A61K48/0058 , A61K48/0066 , C07K14/47 , C12N9/0053 , C12N9/0089 , C12N9/1085 , C12N15/8509 , C12Y109/03001 , C07K2319/07 , C12N2800/22 , C12N2810/80
摘要： An expression vector containing appropriate mitochondrion-targeting sequences (MTS) and appropriate 3′UTR sequences provides efficient and stable delivery of a mRNA encoding a protein (CDS) to the mitochondrion of a mammalian cell. The MTS and 3′UTR sequences guide the CDS mRNA from the nuclear compartment of the cell to mitochondrion-bound polysomes, where the CDS is translated. This provides an efficient translocation of a mature functional protein into the mitochondria. A method of targeting mRNA expressed in the nuclear compartment of a mammalian cell to the mitochondrion is also provided. The vector and methods can be used to treat defects in mitochondrial function.
-
3.发明申请COMBINATION OF A LIGHT RAY WITH A CYTOCHROME C OXIDASE SUBSTRATE PARTICULARLY FOR IMPROVING THE APPEARANCE OF THE SKIN AND/OR HAIR 有权光照与细胞色素C氧化物底物的组合特别用于改善皮肤和/或头发的外观公开(公告)号：US20110046538A1
公开(公告)日：2011-02-24
申请号：US12866575
申请日：2009-02-06
CPC分类号： A61K41/00 , A61K8/19 , A61K8/494 , A61K8/66 , A61K38/1709 , A61K45/06 , A61K2800/81 , A61K2800/92 , A61N5/0617 , A61Q7/00 , A61Q19/007 , A61Q19/02 , A61Q19/08 , C12Y109/03001
摘要： The invention relates to the combination of a light ray with a cytochrome C oxydase substrate particularly for improving the appearance of the skin and/or the hair. The invention more particularly relates to a method for a cosmetic treatment particularly for improving the appearance of the skin and/or the hair that comprises the simultaneous and/or sequential administration of a) at least one cytochrome C oxydase substrate and/or at least one agent increasing the expression of said substrate; and b) at least one light radiation having at least one predominant wavelength that activates the cytochrome C oxydase. More particularly, said light radiation has at least one predominant wavelength activating the cytochrome C oxydase, that ranges from 550 to 1000 nm, in particular from 550 to 800 nm, preferably from 620 to 700 nm, and more preferably from 640 to 680 nm; the method comprises applying a dose preferably ranging from 0.01 to 200 J/cm2, preferably from 0.1 to 30 J/cm2, more preferably from 1 to 30 J/cm2, or even from 5 to 30 J/cm2. The invention also relates to a composition containing at least one cytochrome C oxydase substrate and/or one agent increasing the expression of said substrate, and at least one compound emitting and/or filtering, in particular under light exposure, a light radiation having at least one predominant wavelength that activates the cytochrome C oxydase. The invention also relates to a kit including at least one composition containing at least one cytochrome C oxydase substrate and/or one agent increasing the expression of said substrate, and at least one device and/or compound emitting and/or filtering a light radiation having at least one predominant wavelength that activates the cytochrome C oxydase.
摘要翻译： 本发明涉及光线与细胞色素C氧化酶底物的组合，特别是用于改善皮肤和/或毛发的外观。 本发明更具体涉及用于特别用于改善皮肤和/或毛发外观的化妆品处理方法，其包括同时和/或顺序施用a）至少一种细胞色素C氧化酶底物和/或至少一种 增加所述底物的表达; 和b）至少一种具有至少一种主要波长的光辐射，其激活细胞色素C氧化酶。 更具体地说，所述光辐射具有激活细胞色素C氧化酶的至少一种主要波长，其范围为550至1000nm，特别是550至800nm，优选620至700nm，更优选640至680nm; 该方法包括施加0.01〜200J / cm 2，优选为0.1〜30J / cm 2，更优选为1〜30J / cm 2，或甚至5〜30J / cm 2的剂量。 本发明还涉及包含至少一种细胞色素C氧化酶底物和/或增加所述底物表达的一种试剂的组合物，以及至少一种特别是在光照下发射和/或过滤具有至少 一种主要的波长激活细胞色素C氧化酶。 本发明还涉及一种试剂盒，其包含至少一种含有至少一种细胞色素C氧化酶底物和/或增加所述底物表达的试剂的组合物，以及至少一种发射和/或过滤具有 活化细胞色素C氧化酶的至少一种主要波长。
-
4.发明授权公开(公告)号：US11920153B2
公开(公告)日：2024-03-05
申请号：US16339164
申请日：2017-10-03
发明人： Stefania Rapino , Francesco Zerbetto , Gastone Castellani , Stefano Salvioli , Beatrice Fraboni , Isabella Zironi , Maria Conte , Claudio Franceschi , Daniela Salvatore , Maila Becconi
IPC分类号： C12N5/00 , C12N5/09 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/08 , C12N11/04 , C12N11/06 , C12N11/087 , C12N11/089 , C12N11/12 , G01N33/50
CPC分类号： C12N5/0018 , C12N5/0068 , C12N5/0693 , C12N9/00 , C12N9/0006 , C12N9/0036 , C12N9/0053 , C12N9/0061 , C12N9/0065 , C12N9/0069 , C12N9/0093 , C12N11/04 , C12N11/06 , C12N11/087 , C12N11/089 , C12N11/12 , C12Y101/03004 , C12Y106/03001 , C12Y109/03001 , C12Y110/03002 , C12Y111/01006 , C12Y113/12004 , C12Y117/03002 , G01N33/5008 , C12N2500/02 , C12N2501/71 , C12N2533/30 , C12N2533/50 , C12N2533/54 , C12N2533/70 , C12N2533/72 , C12N2533/74 , C12N2533/76 , C12N2533/78 , C12N2533/80 , C12N2537/10
摘要： The present invention relates to compositions comprising a polymeric matrix or a gel containing functional enzymes capable of re-creating under culture conditions the cell microenvironment existing in vivo. The present invention also relates to devices for cell cultures comprising such compositions, in particular hydrogel and the use thereof to control the chemical microenvironment of a cell culture or mimic physiological or pathological conditions of the in vivo cells. The compositions and the devices described herein could be also used in vitro for evaluating the therapeutic effect of a compound on a determined cell line or on primary cells.
-
公开(公告)号：US20180028491A1
公开(公告)日：2018-02-01
申请号：US15659114
申请日：2017-07-25
发明人： Richard L. JACKSON
IPC分类号： A61K31/353 , C12Q1/48 , C12Q1/68 , C12Q1/26
CPC分类号： A61K31/353 , C12Q1/26 , C12Q1/48 , C12Q1/6883 , C12Q2600/118 , C12Y109/03001 , C12Y203/03001 , G01N2800/2821 , G01N2800/50
摘要： The present invention provides a method for diagnosing or determining the risk of developing Alzheimer's disease and for treating Alzheimer's disease with S-equol. An aspect of the present invention includes the use of a direct mitochondrial target engagement biomarker to diagnose or assess the risk of developing Alzheimer's disease. Another aspect of the present invention includes the use of a pharmaceutically effective amount of S-equol to treat or prevent Alzheimer's disease in a subject diagnosed with or determined to be at risk of developing Alzheimer's disease.
-
6.发明申请公开(公告)号：US20170349885A1
公开(公告)日：2017-12-07
申请号：US15619983
申请日：2017-06-12
发明人： Hiroyuki NAGAOKA
CPC分类号： C12N9/0006 , C12N9/0053 , C12N9/0065 , C12P7/22 , C12P41/002 , C12Y101/05 , C12Y109/03001 , C12Y111/01
摘要： Provided are: a protein complex capable of selectively and asymmetrically oxidizing an enantiomer of a secondary alcohol without adding a coenzyme and having an asymmetric oxidation activity in a water-soluble solvent system in the presence of oxygen; a method for producing the same; and a method for coating the protein complex with a high molecular weight compound. The method for producing the protein complex includes: (1) enclosing a crude water-soluble protein in a gel, air-oxidizing the gel, and eluting the protein complex into an aqueous solution; and (2) applying gravity to concentrate and precipitate the protein complex, redissolving the precipitate in an aqueous glycine sodium hydroxide solution of about 0.5 mM and allowing the same to homogeneously coexist with a high molecular weight compound, and re-precipitating the solution and dehydrating and drying the same to yield a protein complex coated with a high molecular weight compound.
-
公开(公告)号：US09017999B2
公开(公告)日：2015-04-28
申请号：US11913618
申请日：2006-05-03
CPC分类号： C12N15/85 , A61K48/005 , A61K48/0058 , A61K48/0066 , C07K14/47 , C07K2319/07 , C12N9/0053 , C12N9/0089 , C12N9/1085 , C12N15/8509 , C12N2800/22 , C12N2810/80 , C12Y109/03001
摘要： The inventors demonstrate that mRNA sorting to the mitochondrial surface is an efficient way to proceed to such an allotopic expression, and that this mRNA sorting can be controlled by selecting appropriate mitochondrion-targeting sequence (MTS) and appropriate 3′UTR sequences. The CDS sequence which codes for the protein to be delivered into the mitochondrion is guided by these appropriate MTS and 3′UTR sequences from the nuclear compartment to the mitochondrion-bound polysomes (where the CDS is translated), and aids in an efficient translocation of a mature functional protein into the mitochondria. Appropriate MTS and 3′UTR sequences correspond to those of nuclearly-transcribed mitochondrially-targeted mRNAs. The inventors demonstrate that, to obtain a stable therapeutically-effective importation, both an appropriate MTS and an appropriate 3′UTR should preferably be used.
摘要翻译： 本发明人证明，对线粒体表面的mRNA分选是进行这种同种异体表达的有效方式，并且可以通过选择适当的线粒体靶向序列（MTS）和合适的3'UTR序列来控制该mRNA分选。 编码要递送到线粒体中的蛋白质的CDS序列由这些适当的MTS和3'UTR序列从核隔室引导到线粒体结合的多核糖体（其中CDS被翻译），并且有助于有效移位 成熟的功能蛋白进入线粒体。 适当的MTS和3'UTR序列对应于核转录的线粒体靶向mRNA的那些。 发明人证明，为了获得稳定的治疗有效的进口，优选使用适当的MTS和适当的3'UTR。
-
8.发明申请公开(公告)号：US20140377869A1
公开(公告)日：2014-12-25
申请号：US14323240
申请日：2014-07-03
CPC分类号： C12N15/85 , A61K48/005 , A61K48/0058 , A61K48/0066 , C07K14/47 , C07K2319/07 , C12N9/0053 , C12N9/0089 , C12N9/1085 , C12N15/8509 , C12N2800/22 , C12N2810/80 , C12Y109/03001
摘要： An expression vector containing appropriate mitochondrion-targeting sequences (MTS) and appropriate 3′UTR sequences provides efficient and stable delivery of a mRNA encoding a protein (CDS) to the mitochondrion of a mammalian cell. The MTS and 3′UTR sequences guide the CDS mRNA from the nuclear compartment of the cell to mitochondrion-bound polysomes, where the CDS is translated. This provides an efficient translocation of a mature functional protein into the mitochondria. A method of targeting mRNA expressed in the nuclear compartment of a mammalian cell to the mitochondrion is also provided. The vector and methods can be used to treat defects in mitochondrial function.
摘要翻译： 包含适当的线粒体靶向序列（MTS）和适当的3'UTR序列的表达载体提供编码蛋白质（CDS）的mRNA到哺乳动物细胞的线粒体的有效且稳定的递送。 MTS和3'UTR序列将CDS mRNA从细胞的核腔引导到线粒体结合的多核糖体，其中CDS被翻译。 这提供了成熟功能蛋白质有效转运到线粒体中。 还提供了将在哺乳动物细胞的核隔室中表达的mRNA靶向线粒体的方法。 载体和方法可用于治疗线粒体功能缺陷。
-
9.发明授权Compositions and methods for the diagnosis and treatment of inflammatory disorders and fibrotic disease 失效用于诊断和治疗炎性疾病和纤维化疾病的组合物和方法公开(公告)号：US08486909B2
公开(公告)日：2013-07-16
申请号：US13380320
申请日：2010-06-24
申请人： Stephen I. Rennard , Tadashi Sato , Xiang-der Liu , Olaf Holz , Helgo Magnussen
发明人： Stephen I. Rennard , Tadashi Sato , Xiang-der Liu , Olaf Holz , Helgo Magnussen
IPC分类号： A61K48/00
CPC分类号： C12N15/1137 , C12N2310/141 , C12Y109/03001
摘要： Compositions and methods are disclosed for the treatment and diagnosis of inflammatory diseases and disorders, including pulmonary diseases and fibrotic disorders, including COPD.
摘要翻译： 公开了用于治疗和诊断炎性疾病和病症（包括肺部疾病和纤维化疾病，包括COPD）的组合物和方法。
-
公开(公告)号：US11779657B2
公开(公告)日：2023-10-10
申请号：US16307128
申请日：2017-06-09
发明人： John Burnett , Anh Pham
IPC分类号： A61K48/00 , C12N15/86 , C12N15/52 , A61K9/51 , C12N15/113 , C12N9/22 , C12N15/10 , C12N15/88
CPC分类号： A61K48/0058 , A61K9/51 , A61K48/0066 , A61K48/0091 , C12N9/22 , C12N15/102 , C12N15/113 , C12N15/1137 , C12N15/52 , C12N15/86 , C07K2319/07 , C12N15/88 , C12N2310/20 , C12N2310/3517 , C12N2310/3519 , C12N2330/51 , C12N2710/10343 , C12N2740/16043 , C12N2750/14143 , C12Y109/03001 , C12Y301/27007
摘要： Compositions and methods for mitochondria genome editing are provided. Also provided are methods for treating mitochondrial disorders by the disclosed compositions.
-
-
-
-
-
-
-
-
-
搜索结果
34 条记录 (耗时 0.023 秒)
