公开(公告)号：US20050261483A1

公开(公告)日：2005-11-24

申请号：US11058727

申请日：2005-02-15

申请人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Theodore Kahn ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  James Wong ,  Cao-Guo Yu

发明人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Theodore Kahn ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  James Wong ,  Cao-Guo Yu

IPC分类号： C07K14/325 ,  C12N15/32 ,  C12N15/82 ,  G06F19/00 ,  C07K14/195

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

公开(公告)号：US20050261188A1

公开(公告)日：2005-11-24

申请号：US11108389

申请日：2005-04-18

申请人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Theodore Kahn ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  James Wong ,  Cao-Guo Yu

发明人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Theodore Kahn ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  James Wong ,  Cao-Guo Yu

IPC分类号： C07K14/325 ,  C12N15/32 ,  C12N15/82 ,  G06F19/00 ,  A01N63/00

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

摘要翻译： 本发明提供从编码具有杀虫活性的昆虫害虫的δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段。 本发明进一步提供已被修饰以编码杀虫多肽的诱变核酸，例如具有改善的杀虫活性和/或改变的有害生物特异性的内毒素。 本发明的具体实施方案提供编码可能被优化的杀虫蛋白质的分离的核酸，以及包含本发明的核酸的农药组合物，表达盒和转化的微生物和植物。 这些组合物可用于防治害虫，特别是植物害虫的方法。

公开(公告)号：US20050166284A1

公开(公告)日：2005-07-28

申请号：US11021115

申请日：2004-12-22

申请人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janet Rice ,  James Wong ,  Cao-Guo Yu

发明人： Andre Abad ,  Ronald Flannagan ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janet Rice ,  James Wong ,  Cao-Guo Yu

IPC分类号： A01H1/00 ,  A01H5/00 ,  C07K14/325 ,  C12N9/64 ,  C12N15/82

CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

摘要翻译： 提供了保护植物免受害虫害虫的组合物和方法。 特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。 通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。 提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。 本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

公开(公告)号：US20080108127A1

公开(公告)日：2008-05-08

申请号：US11950509

申请日：2007-12-05

申请人： Andre Abad ,  Ronald Flannagan ,  Rafael Hermann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janet Rice ,  James Wong ,  Cao-Guo Yu

发明人： Andre Abad ,  Ronald Flannagan ,  Rafael Hermann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janet Rice ,  James Wong ,  Cao-Guo Yu

IPC分类号： C07K16/00 ,  C12N9/50

CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

公开(公告)号：US20050188440A1

公开(公告)日：2005-08-25

申请号：US11093088

申请日：2005-03-29

申请人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

发明人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N7/01 ,  C12N15/12 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N7/00

CPC分类号： C12N15/8282 ,  C07K14/43563 ,  Y10S530/858

摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

摘要翻译： 本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。 本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。 通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。 进一步提供了用本发明的核酸分子转化的植物细胞，植物和种子，以赋予植物抗病性。

公开(公告)号：US20050166287A1

公开(公告)日：2005-07-28

申请号：US11092427

申请日：2005-03-29

申请人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

发明人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N7/01 ,  C12N15/12 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C12N7/00

CPC分类号： C12N15/8282 ,  C07K14/43563 ,  Y10S530/858

摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

摘要翻译： 本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。 本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。 通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。 进一步提供了用本发明的核酸分子转化的植物细胞，植物和种子，以赋予植物抗病性。

公开(公告)号：US20050166286A1

公开(公告)日：2005-07-28

申请号：US11092027

申请日：2005-03-29

申请人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

发明人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N7/01 ,  C12N15/12 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C12N7/00 ,  C12N15/87

CPC分类号： C12N15/8282 ,  C07K14/43563 ,  Y10S530/858

摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

摘要翻译： 本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。 本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。 通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。 进一步提供了用本发明的核酸分子转化的植物细胞，植物和种子，以赋予植物抗病性。

公开(公告)号：US20050164940A1

公开(公告)日：2005-07-28

申请号：US11092430

申请日：2005-03-29

申请人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

发明人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N7/01 ,  C12N15/12 ,  C12N15/82 ,  A61K38/16 ,  A01H5/00 ,  C07K14/415

CPC分类号： C12N15/8282 ,  C07K14/43563 ,  Y10S530/858

摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

摘要翻译： 本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。 本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。 通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。 进一步提供了用本发明的核酸分子转化的植物细胞，植物和种子，以赋予植物抗病性。

公开(公告)号：US20050164939A1

公开(公告)日：2005-07-28

申请号：US11092429

申请日：2005-03-29

申请人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

发明人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N7/01 ,  C12N15/12 ,  C12N15/82 ,  A61K38/16 ,  A01H5/00 ,  C07K14/415

CPC分类号： C12N15/8282 ,  C07K14/43563 ,  Y10S530/858

摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

摘要翻译： 本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。 本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。 通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。 进一步提供了用本发明的核酸分子转化的植物细胞，植物和种子，以赋予植物抗病性。

公开(公告)号：US20050164941A1

公开(公告)日：2005-07-28

申请号：US11093225

申请日：2005-03-29

申请人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

发明人： Daniel Altier ,  Rafael Herrmann ,  Albert Lu ,  Billy McCutchen ,  James Presnail ,  Janine Weaver ,  James Wong

IPC分类号： C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N7/01 ,  C12N15/12 ,  C12N15/82 ,  A61K38/16 ,  A01H5/00 ,  C07K14/415

CPC分类号： C12N15/8282 ,  C07K14/43563 ,  Y10S530/858

摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, and seed thereof, transformed with the nucleic acid molecules of the invention so as to confer disease resistance on the plant.

摘要翻译： 本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。 本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。 通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。 进一步提供了用本发明的核酸分子转化的植物细胞，植物和种子，以赋予植物抗病性。