公开(公告)号：US20050009026A1

公开(公告)日：2005-01-13

申请号：US10487915

申请日：2002-08-24

申请人： Andreas Abel ,  Markus Ehrat ,  Ekkehard Kauffmann ,  Dominic Utinger ,  Vincent Benoit ,  Susan De Paul ,  Marcus Textor ,  Stephanie Vande Vondele ,  Jeffrey Hubbell

发明人： Andreas Abel ,  Markus Ehrat ,  Ekkehard Kauffmann ,  Dominic Utinger ,  Vincent Benoit ,  Susan De Paul ,  Marcus Textor ,  Stephanie Vande Vondele ,  Jeffrey Hubbell

IPC分类号： C12Q1/68 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00 ,  A61L2/00 ,  C07H21/04 ,  C12M1/34

CPC分类号： C12Q1/6837 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00387 ,  B01J2219/00529 ,  B01J2219/0054 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00

摘要： The invention relates to a surface for the immobilization of one or several first nucleic acids as recognition elements (“immobilization surface”), for the production of a recognition surface for the detection of one or several second nucleic acids in one or more samples which are brought into contact with the recognition surface, the first nucleic acids being applied to a layer of the graft copolymer poly(L-lysine)-g-poly(ethyleneglycol) (PLL-g-PEG) as surface for immobilization, characterized in that the grafting ratio g, in other words the ratio between the number of lysine units and the number of polyethylene glycol side chains (“PEG” side chains) has an average value between 7 and 13. The invention also relates to a method for the qualitative and/or quantitative detection of one or more second nucleic acids in one or more samples, characterized in that said samples and optionally further reagents are brought into contact with an immobilization surface according to the invention, upon which one or several first nucleic acids are immobilized as recognition elements for specific binding/hybridization with said second nucleic acids and changes in optical or electronic signals resulting from the binding/hybridization of said second nucleic acid, or further, resulting from applied tracer substances applied for analyte detection, are measured.

摘要翻译： 本发明涉及用于将一个或几个第一核酸作为识别元件（“固定表面”）固定的表面，用于产生用于检测一个或多个样品中一个或多个第二核酸的识别表面，所述一个或多个第二核酸是 与识别表面接触，将第一核酸施加到作为用于固定的表面的接枝共聚物聚（L-赖氨酸）-g-聚（乙二醇）（PLL-g-PEG）的层上，其特征在于， 接枝率g，换句话说，赖氨酸单元数与聚乙二醇侧链（“PEG”侧链）的数量之间的平均值为7-13之间。本发明还涉及一种定性和 /或一个或多个样品中一种或多种第二核酸的定量检测，其特征在于所述样品和任选的其它试剂与根据本发明的固定化表面接触，u pon，其中一个或几个第一核酸被固定为用于与所述第二核酸特异性结合/杂交的识别元件，以及由所述第二核酸的结合/杂交产生的光学或电子信号的变化，或者进一步由施用的示踪物质 应用于分析物检测。

公开(公告)号：US4860365A

公开(公告)日：1989-08-22

申请号：US110938

申请日：1987-10-21

申请人： Clement Eloise-Pillerault ,  Claude Benoit ,  Vincent Benoit

发明人： Clement Eloise-Pillerault ,  Claude Benoit ,  Vincent Benoit

IPC分类号： H04R1/26 ,  H04R3/12

CPC分类号： H04R1/26 ,  H04R3/12

摘要： The invention relates to a method for selectively restoring a sound signal delivered by an electric source in several parallel mounted transducers, each transducer being specialized for restoring a frequency range of the spectrum of the signal. The method consists more precisely in permanently fractionating the sound signal into frequencies so as to assign each component to the transducer optimized for the corresponding frequency range, without using any additional selection circuit such as filtering circuits.

摘要翻译： 本发明涉及一种用于在几个并联安装的换能器中选择性地恢复由电源传送的声音信号的方法，每个换能器专用于恢复信号频谱的频率范围。 该方法更精确地包括将声音信号永久分割成频率，以便将每个分量分配给针对相应频率范围优化的传感器，而不使用任何附加的选择电路，例如滤波电路。

公开(公告)号：US09170265B2

公开(公告)日：2015-10-27

申请号：US13128543

申请日：2009-11-12

申请人： Vincent Benoit

发明人： Vincent Benoit

IPC分类号： A61B5/05 ,  G01N33/72 ,  G01N27/327

CPC分类号： G01N33/725 ,  G01N27/3271 ,  G01N33/726

摘要： A method for amperometric detection of proteins, especially haemoglobin in faeces, using an electrochemical sensor. The electrochemical sensor includes: a working electrode having an electrically conductive matrix holding a first reagent and/or a second reagent, the second reagent being an oxidizing agent, or a precursor thereof, for the first reagent; a counter electrode and optionally a reference electrode; wherein a reaction between the first reagent and the oxidizing agent is catalyzed by the protein to provide a detectable signal at the working electrode. The electrically conductive matrix is an electrically conductive carbon- or graphite-containing matrix or an electrically conductive porous matrix.

摘要翻译： 使用电化学传感器对蛋白质，特别是粪便中的血红蛋白进行电流检测的方法。 电化学传感器包括：用于第一试剂的工作电极，其具有保持第一试剂和/或第二试剂的导电基质，第二试剂为氧化剂或其前体; 对电极和任选的参考电极; 其中所述第一试剂和所述氧化剂之间的反应被所述蛋白质催化以在所述工作电极处提供可检测的信号。 导电基体是导电的含碳或石墨的基体或导电多孔基体。

公开(公告)号：US20110213229A1

公开(公告)日：2011-09-01

申请号：US13128543

申请日：2009-11-12

申请人： Vincent Benoit

发明人： Vincent Benoit

IPC分类号： A61B5/00 ,  G01N27/403 ,  G01N27/416 ,  H01B1/00 ,  H01B1/04

CPC分类号： G01N33/725 ,  G01N27/3271 ,  G01N33/726

摘要： A method for amperometric detection of proteins, especially haemoglobin in faeces, using an electrochemical sensor. The electrochemical sensor includes: a working electrode having an electrically conductive matrix holding a first reagent and/or a second reagent, the second reagent being an oxidising agent, or a precursor thereof, for the first reagent; a counter electrode and optionally a reference electrode; wherein a reaction between the first reagent and the oxidising agent is catalysed by the protein to provide a detectable signal at the working electrode. The electrically conductive matrix is an electrically conductive carbon- or graphite-containing matrix or an electrically conductive porous matrix.

摘要翻译： 使用电化学传感器对蛋白质，特别是粪便中的血红蛋白进行电流检测的方法。 电化学传感器包括：用于第一试剂的工作电极，其具有保持第一试剂和/或第二试剂的导电基质，第二试剂为氧化剂或其前体; 对电极和任选的参考电极; 其中所述第一试剂和所述氧化剂之间的反应由所述蛋白质催化以在所述工作电极处提供可检测信号。 导电基体是导电的含碳或石墨的基体或导电多孔基体。