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    Surface for the immobilisation of nucleic acids
    1.
    发明申请
    Surface for the immobilisation of nucleic acids 审中-公开
    用于固定核酸的表面

    公开(公告)号：US20050009026A1

    公开(公告)日：2005-01-13

    申请号：US10487915

    申请日：2002-08-24

    申请人： Andreas Abel ,  Markus Ehrat ,  Ekkehard Kauffmann ,  Dominic Utinger ,  Vincent Benoit ,  Susan De Paul ,  Marcus Textor ,  Stephanie Vande Vondele ,  Jeffrey Hubbell

    发明人： Andreas Abel ,  Markus Ehrat ,  Ekkehard Kauffmann ,  Dominic Utinger ,  Vincent Benoit ,  Susan De Paul ,  Marcus Textor ,  Stephanie Vande Vondele ,  Jeffrey Hubbell

    IPC分类号： C12Q1/68 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00 ,  A61L2/00 ,  C07H21/04 ,  C12M1/34

    CPC分类号： C12Q1/6837 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00387 ,  B01J2219/00529 ,  B01J2219/0054 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00

    摘要： The invention relates to a surface for the immobilization of one or several first nucleic acids as recognition elements (“immobilization surface”), for the production of a recognition surface for the detection of one or several second nucleic acids in one or more samples which are brought into contact with the recognition surface, the first nucleic acids being applied to a layer of the graft copolymer poly(L-lysine)-g-poly(ethyleneglycol) (PLL-g-PEG) as surface for immobilization, characterized in that the grafting ratio g, in other words the ratio between the number of lysine units and the number of polyethylene glycol side chains (“PEG” side chains) has an average value between 7 and 13. The invention also relates to a method for the qualitative and/or quantitative detection of one or more second nucleic acids in one or more samples, characterized in that said samples and optionally further reagents are brought into contact with an immobilization surface according to the invention, upon which one or several first nucleic acids are immobilized as recognition elements for specific binding/hybridization with said second nucleic acids and changes in optical or electronic signals resulting from the binding/hybridization of said second nucleic acid, or further, resulting from applied tracer substances applied for analyte detection, are measured.

    摘要翻译： 本发明涉及用于将一个或几个第一核酸作为识别元件（“固定表面”）固定的表面，用于产生用于检测一个或多个样品中一个或多个第二核酸的识别表面，所述一个或多个第二核酸是 与识别表面接触，将第一核酸施加到作为用于固定的表面的接枝共聚物聚（L-赖氨酸）-g-聚（乙二醇）（PLL-g-PEG）的层上，其特征在于， 接枝率g，换句话说，赖氨酸单元数与聚乙二醇侧链（“PEG”侧链）的数量之间的平均值为7-13之间。本发明还涉及一种定性和 /或一个或多个样品中一种或多种第二核酸的定量检测，其特征在于所述样品和任选的其它试剂与根据本发明的固定化表面接触，u pon，其中一个或几个第一核酸被固定为用于与所述第二核酸特异性结合/杂交的识别元件，以及由所述第二核酸的结合/杂交产生的光学或电子信号的变化，或者进一步由施用的示踪物质 应用于分析物检测。