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公开(公告)号：US09040243B2
公开(公告)日：2015-05-26
申请号：US13825226
申请日：2011-09-22
CPC分类号： C12Q1/6853 , C12Q1/6846 , C12Q1/6851 , C12Q1/686 , C12Q1/6865 , C12Q2537/157 , C12Q2525/151 , C12Q2545/114 , C12Q2561/113
摘要： The present invention relates to a method, kit and use of various nucleic acid sequences for deleting and/or quantifying one or more nucleic acids of a genome in a sample. Wherein the nucleic acid is amplified and the locus that is amplified is a multi copy locus within the genome, the multicopy locus has copies on at least two different chromosomes and the amplification product is detected and/or quantified.
摘要翻译： 本发明涉及用于缺失和/或定量样品中一个或多个基因组核酸的各种核酸序列的方法，试剂盒和用途。 其中核酸被扩增并且扩增的基因座是基因组内的多拷贝基因座，多拷贝基因座在至少两个不同染色体上具有拷贝，并且检测和/或定量扩增产物。
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公开(公告)号：US20130224742A1
公开(公告)日：2013-08-29
申请号：US13825226
申请日：2011-09-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12Q1/6846 , C12Q1/6851 , C12Q1/686 , C12Q1/6865 , C12Q2537/157 , C12Q2525/151 , C12Q2545/114 , C12Q2561/113
摘要： The present invention relates to a method, kit and use of various nucleic acid sequences for deleting and/or quantifying one or more nucleic acids of a genome in a sample. Wherein the nucleic acid is amplified and the locus that is amplified is a multi copy locus within the genome, the multicopy locus has copies on at least two different chromosomes and the amplification product is detected and/or quantified.
摘要翻译： 本发明涉及用于缺失和/或定量样品中一个或多个基因组核酸的各种核酸序列的方法，试剂盒和用途。 其中核酸被扩增并且扩增的基因座是基因组内的多拷贝基因座，多拷贝基因座在至少两个不同染色体上具有拷贝，并且检测和/或定量扩增产物。
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公开(公告)号：US08852863B2
公开(公告)日：2014-10-07
申请号：US13318424
申请日：2010-05-04
申请人： Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wende , Rainer Dahlke
发明人： Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wende , Rainer Dahlke
CPC分类号： C12Q1/6818 , Y02A50/59 , C12Q2527/107 , C12Q2537/143 , C12Q2565/629
摘要： The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.
摘要翻译： 本发明涉及用于扩增和检测反应盒中的核酸序列的方法，其包括：（i）提供包含至少一个核酸分子的样品，（ii）在所述盒的第一反应室中提供用于扩增的试剂 反应，（iii）将样品与扩增试剂混合，（iv）扩增筒的第一反应室中的至少一种核酸，（v）将至少部分扩增反应转移到第二和第三反应室 的每个包含探针组并进行熔点分析，以便确定哪个探针具体结合核酸。
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4.发明申请PROCESS FOR AMPLIFYING DNA USING TETRATETHYLENE GLYCOL, KIT OF PARTS THEREFOR AND USE THEREOF 审中-公开使用四乙二醇甘氨酸放大DNA的方法及其部件及其用途公开(公告)号：US20120171728A1
公开(公告)日：2012-07-05
申请号：US13380002
申请日：2010-06-15
申请人： Andy Wende , Hans Robert Attig , Ralf Himmelreich
发明人： Andy Wende , Hans Robert Attig , Ralf Himmelreich
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q1/686 , C12Q2527/125
摘要： Disclosed is a process for amplifying DNA, a process for preparing and amplifying DNA, a kit of parts for DNA amplification and DNA preparation, and the use thereof, all of which are characterized by the use of tetraethylene glycol. Moreover, the use of tetraethylene glycol in a reaction solution by carrying out a DNA amplification and a reaction solution containing tetraethylene glycol for carrying out a DNA amplification are disclosed.
摘要翻译： 公开了扩增DNA的方法，DNA的制备和扩增方法，DNA扩增和DNA制备的试剂盒及其用途，其特征在于使用四甘醇。 此外，公开了通过进行DNA扩增反应溶液中的四甘醇和含有四甘醇的反应液进行DNA扩增。
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