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    Detection of multiple nucleic acid sequences in a reaction cartridge
    2.
    发明授权
    Detection of multiple nucleic acid sequences in a reaction cartridge 有权
    检测反应筒中的多个核酸序列

    公开(公告)号：US08852863B2

    公开(公告)日：2014-10-07

    申请号：US13318424

    申请日：2010-05-04

    申请人： Thomas Rothmann ,  Holger Engel ,  Ralf Himmelreich ,  Andy Wende ,  Rainer Dahlke

    发明人： Thomas Rothmann ,  Holger Engel ,  Ralf Himmelreich ,  Andy Wende ,  Rainer Dahlke

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6818 ,  Y02A50/59 ,  C12Q2527/107 ,  C12Q2537/143 ,  C12Q2565/629

    摘要： The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.

    摘要翻译： 本发明涉及用于扩增和检测反应盒中的核酸序列的方法，其包括：（i）提供包含至少一个核酸分子的样品，（ii）在所述盒的第一反应室中提供用于扩增的试剂 反应，（iii）将样品与扩增试剂混合，（iv）扩增筒的第一反应室中的至少一种核酸，（v）将至少部分扩增反应转移到第二和第三反应室 的每个包含探针组并进行熔点分析，以便确定哪个探针具体结合核酸。

    HELICASE DEPENDENT ISOTHERMAL AMPLIFICATION USING NICKING ENZYMES
    4.
    发明申请
    HELICASE DEPENDENT ISOTHERMAL AMPLIFICATION USING NICKING ENZYMES 有权
    使用镍化合物进行依赖相同的等温放大

    公开(公告)号：US20130210019A1

    公开(公告)日：2013-08-15

    申请号：US13817174

    申请日：2011-08-16

    申请人： Christian Korfhage ,  Gerd Grosshauser ,  Thomas Rothmann ,  Ralf Himmelreich

    发明人： Christian Korfhage ,  Gerd Grosshauser ,  Thomas Rothmann ,  Ralf Himmelreich

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q2521/301 ,  C12Q2521/513

    摘要： The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.

    摘要翻译： 本发明涉及用于扩增模板核酸的方法，其中所述方法包括在切口内切核酸酶存在下使用解旋酶依赖性扩增（HDA）反应扩增所述模板核酸，并且其中所述模板核酸包含识别的序列 通过所述切口内切核酸酶或由所述切口内切核酸酶识别的序列在HDA反应期间引入模板核酸。 本发明还涉及用于扩增核酸的试剂盒，其包含切口内切核酸酶，解旋酶和DNA聚合酶。

    Method for the treatment of a sample containing biomolecules
    7.
    发明授权
    Method for the treatment of a sample containing biomolecules 有权
    用于处理含有生物分子的样品的方法

    公开(公告)号：US08076069B2

    公开(公告)日：2011-12-13

    申请号：US12477813

    申请日：2009-06-03

    申请人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    发明人： Christian Korfhage ,  Friederike Wilmer ,  Dirk Löffert ,  Ralf Himmelreich ,  Claudia Fritz ,  Kathleen Rieske

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C12N1/06 ,  C12N15/1003 ,  C12Q1/6806 ,  G01N33/6803 ,  C12Q2531/113 ,  C12Q2527/137 ,  C12Q2527/125

    摘要： The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymers, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more of these compounds. The invention provides in particular a method for the lysis of a biological sample, and methods for the stabilization of biomolecules, a method for the decrease of inhibiting effects in a sample containing biomolecules and a differential masking method.

    摘要翻译： 本发明通常提供了用于样品制备用于随后的含有至少一种核酸和/或一种蛋白质的样品的制备，加工或分析方法的方法，其中所述方法包括以下步骤：A）提供 包含至少一种核酸和/或蛋白质的样品，B）使样品与流体或固体组合物接触以产生流体样品制备物，其中所述组合物含有至少一种选自氮的化合物 包括a）多胺，b）氨基酸和寡聚和多肽，c）包含这些含氮化合物的含氮杂环化合物，包括均相杂聚物，d）R1R2NR3型胺，其中R1，R2 和R 3彼此独立地选自H，C 1 -C 5烷基和芳基，其中R 1，R 2和R 3不同时为H，e）羧酸酰胺，f ）无机铵盐，g）含有内盐化合物的铵基，h）抗生素结合核酸，i）结合在DNA的小腔中的化合物，以及两种或更多种这些化合物的混合物。 本发明特别提供了生物样品的裂解方法，以及用于稳定生物分子的方法，降低含有生物分子的样品中的抑制作用的方法和差示掩蔽法。