公开(公告)号：US07320872B2

公开(公告)日：2008-01-22

申请号：US10183728

申请日：2002-06-27

申请人： Ann Marie Janson ,  Jonas Frisen ,  Clas Johansson ,  Stefan Momma ,  Diana Clarke ,  Ming Zhao ,  Urban Lendahl ,  Kioumars Delfani

发明人： Ann Marie Janson ,  Jonas Frisen ,  Clas Johansson ,  Stefan Momma ,  Diana Clarke ,  Ming Zhao ,  Urban Lendahl ,  Kioumars Delfani

IPC分类号： G01N33/567

CPC分类号： C12N5/0623 ,  A61K35/12

摘要： The invention relates to an ependymal neural CNS stem cell, which cell expresses the surface protein Notch 1 together with at least one surface protein chosen from the group of Notch 2, Notch 3, CAR (transmembrane protein binding adenovirus) and CFTR cystic fibrosis transmembrane conductance regulator), and which cell also comprises at least one cilium. The invention also relates to preparations, including pharmaceutical preparations, comprising ependymal neural CNS stem cells, in vitro and in vivo assays based thereon and various other uses of the novel ependymal cells according to the invention.

摘要翻译： 本发明涉及室管膜神经CNS干细胞，该细胞表达蛋白Notch1以及至少一种选自Notch 2，Notch 3，CAR（跨膜蛋白结合腺病毒）和CFTR囊性纤维化跨膜电导的表面蛋白 调节剂），哪个细胞还包含至少一个纤毛。 本发明还涉及包括室管膜神经CNS干细胞的药物制剂，其中基于其的体外和体内测定法以及根据本发明的新型室管膜细胞的各种其它用途的制剂。

公开(公告)号：US07279332B2

公开(公告)日：2007-10-09

申请号：US10326438

申请日：2002-12-20

申请人： Jonas Frisén ,  Ann Marie Janson ,  Clas Johansson ,  Stefan Momma ,  Diana Clarke ,  Ming Zhao ,  Urban Lendahl ,  Kioumars Delfani

发明人： Jonas Frisén ,  Ann Marie Janson ,  Clas Johansson ,  Stefan Momma ,  Diana Clarke ,  Ming Zhao ,  Urban Lendahl ,  Kioumars Delfani

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/08

CPC分类号： C12N5/0623 ,  C12N2510/00

摘要： The present invention relates to a method of determining whether a test compound is a proliferation-inducing or proliferation-inhibiting agent of ependymal neural stem cells. The method comprises: (a) contacting an ependymal neural stem cell with the test compound: and (b) determining the proliferation of the ependymal neural stem cell, wherein if the proliferation of the ependymal neural stem cell is increased in the presence of the test compound as compared to the absence of the test compound, the test compound is identified as a proliferation-inducing agent or wherein if the proliferation of the ependymal neural stem cell is decreased in the presence of the test compound as compared to the absence of the test compound, the test compound is identified as a proliferation-inhibiting agent.

摘要翻译： 本发明涉及一种测定化合物是否是室管膜神经干细胞增殖诱导或增殖抑制剂的方法。 该方法包括：（a）将室管膜神经干细胞与测试化合物接触：（b）确定室管膜神经干细胞的增殖，其中如果在试验存在的情况下增加室管膜神经干细胞的增殖 化合物与不存在测试化合物相比，被测化合物被鉴定为增殖诱导剂，或者如果在不存在测试的情况下，在测试化合物存在的情况下降低室管膜神经干细胞的增殖 化合物，测试化合物被鉴定为增殖抑制剂。

公开(公告)号：US06541247B1

公开(公告)日：2003-04-01

申请号：US09104772

申请日：1998-06-25

申请人： Jonas Frisën ,  Ann Marie Janson ,  Clas Johansson ,  Stefan Momma ,  Diana Clarke ,  Ming Zhao ,  Urban Lendahl ,  Kioumars Delfani

发明人： Jonas Frisën ,  Ann Marie Janson ,  Clas Johansson ,  Stefan Momma ,  Diana Clarke ,  Ming Zhao ,  Urban Lendahl ,  Kioumars Delfani

IPC分类号： C12N500

CPC分类号： C12N5/0623 ,  C12N2510/00

摘要： The present invention relates to a method of isolating ependymal neural CNS stem cells from a post-natal animal or a human, which method comprises the steps of (a) screening single cells obtained by dissociating CNS tissue from said animal for cells exhibiting at least one characteristic of an ependymal neural stem cell; and (b) recovering the cells that exhibit the characteristic or characteristics screened for in step (a). The screening may be performed for a specific cell surface protein or by previously labeling the ependymal cells. The invention also relates to isolated ependymal neural CNS stem cells, in vitro and in vivo assays based on the findings according to the invention and various uses of the ependymal neural stem cells according to the invention.

摘要翻译： 本发明涉及一种从产后动物或人体分离室管膜神经CNS干细胞的方法，该方法包括以下步骤：（a）筛选通过从所述动物解离CNS组织获得的细胞，所述单细胞显示至少一种 室管膜神经干细胞的特征; （b）回收表现出在步骤（a）中筛选的特征或特征的细胞。可以对特定的细胞表面蛋白进行筛选或通过预先标记室管膜细胞进行筛选。本发明还涉及分离的室管膜神经中枢神经系统干细胞 基于根据本发明的发现和根据本发明的室管膜神经干细胞的各种用途的体外和体内测定。

公开(公告)号：US20060222634A1

公开(公告)日：2006-10-05

申请号：US11392892

申请日：2006-03-29

申请人： Diana Clarke ,  Charlotte Smith ,  Richard Banas ,  Vivienne Marshall

发明人： Diana Clarke ,  Charlotte Smith ,  Richard Banas ,  Vivienne Marshall

IPC分类号： A01N63/00 ,  C12N5/00

CPC分类号： A61K35/50 ,  A61K8/982 ,  A61K35/12 ,  A61K2800/10 ,  A61Q19/00 ,  C12N5/0605 ,  C12N5/0629 ,  C12N2501/11 ,  C12N2502/02

摘要： The invention is directed to substantially purified amnion-derived cell populations, compositions comprising the substantially purified amnion-derived cell populations, and to methods of creating such substantially purified amnion-derived cell populations, as well as methods of use. The invention is further directed to antibodies, in particular, monoclonal antibodies, that bind to amnion-derived cells or, alternatively, to one or more amnion-derived cell surface protein markers. The invention is further directed to methods for producing the antibodies, methods for using the antibodies, and kits comprising the antibodies.