公开(公告)号：US20030152994A1

公开(公告)日：2003-08-14

申请号：US10373450

申请日：2003-02-24

Applicant: Applera Corporation

Inventor： Timothy W. Woudenberg ,  Michael Albin ,  Reid B. Kowallis ,  Yefim Raysberg ,  Robert P. Ragusa ,  Emily S. Winn-Deen

IPC: C12Q001/68 ,  C12M001/34

CPC classification number: B01L3/5027 ,  B01L3/5025 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0642 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/049 ,  C12Q1/08 ,  G01N35/08 ,  G01N35/10 ,  G01N2035/00148 ,  G01N2035/00356 ,  G01N2035/1032 ,  G01N2035/1034 ,  Y10S436/807

Abstract: The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Abstract translation: 本发明涉及一种用于同时测试样品的一种或多种选择的分析物的存在，不存在和/或量的方法和装置。 本发明在一个方面包括用于检测或定量液体样品中的多种不同分析物的装置。 该装置包括限定样品分配网络的基板，其具有（i）样品入口，（ii）一个或多个检测室，以及（iii）通道装置，其在每个室之间提供死端流体连接，并且入口 。 每个室可以包括有效地与可能存在于样品中的选定分析物反应的分析物特异性试剂和用于检测信号的检测装置。 还公开了利用该装置的方法。