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1.发明公开公开(公告)号：US20240011019A1
公开(公告)日：2024-01-11
申请号：US18348201
申请日：2023-07-06
发明人： Joshua LaBaer
CPC分类号： C12N15/1062 , G01N33/6845 , G01N33/6854 , G01N2458/10
摘要： Methods and compositions for multiplexed protein-protein interaction profiling (e.g., immunoprofiling), based on nucleic acid tagging of polypeptides (e.g., by RNA display) are described. In some embodiments the described compositions and methods utilize a library of prey polypeptide targets linked to prey RNAs encoding them, and a population of bait polypeptides, e.g., a mixture of antibodies, that bind to one or more of the prey polypeptide targets and are used to isolate and identify the bound prey polypeptide targets by amplification of their associated prey RNAs and sequencing of the corresponding cDNAs. In other embodiments the prey polypeptide targets are linked to DNA Bar Codes, which serve as unique identifiers of the tagged polypeptide.
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公开(公告)号：US11525831B2
公开(公告)日：2022-12-13
申请号：US16743906
申请日：2020-01-15
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY , THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
发明人： Joshua LaBaer , Jie Wang , Ji Qui , Garrick Wallstrom , Karen Anderson , Jin Park , Jonine Figueroa
IPC分类号： G01N33/564 , G01N33/574 , C07K16/30 , C12Q1/6886 , C12N15/11
摘要： Cancer patients make antibodies to tumor-derived proteins that are potential biomarkers for early detection. Twenty-eight antigens have been identified as potential biomarkers for the early detection of basal-like breast cancer (Tables 1, 2). Also, a 13-AAb classifier has been developed that differentiate patients with BLBC from healthy controls with 33% sensitivity at 98% specificity (Table 3).
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公开(公告)号：US11243208B2
公开(公告)日：2022-02-08
申请号：US16317184
申请日：2017-07-11
申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY , THE BRIGHAM AND WOMEN'S HOSPITAL, INC.
IPC分类号： G01N31/00 , G01N33/53 , G01N33/574
摘要： Compositions and methods relating to a panel of antigen biomarkers for the early detection of ovarian cancer. The compositions and methods encompass antigen biomarkers coupled to a substrate, with the biomarkers being selected from the group consisting of one or more of ICAM3, CTAG2, p53, STYXL1, PVR, POMC, NUDT11, TRIM39, UHMK1, KSR1, and NXF3.
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4.发明申请公开(公告)号：US20210381028A1
公开(公告)日：2021-12-09
申请号：US17346008
申请日：2021-06-11
申请人： Arizona Board of Regents on Behalf of Arizona State University , Mayo Foundation for Medical Education and Research
发明人： Joshua LaBaer , Vel Murugan , Merica Vorachitti , Rahul Pannala , Douglas Faigel
IPC分类号： C12Q1/6806 , C12Q1/6851 , C12Q1/689
摘要： Provided herein are qPCR-based methods for quantitatively detecting and differentiating between live and dead pathogenic bacteria such as carbapenem-resistant Enterobacteriaceae (CRE). Also provided herein are simple, fast, and reliable genomic DNA extraction methods with improved efficiency.
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公开(公告)号：US20190302122A1
公开(公告)日：2019-10-03
申请号：US16317184
申请日：2017-07-11
申请人： Benjamin KATCHMAN , Karen ANDERSON , Garrick WALLSTROM , Joshua LABAER , Daniel CRAMER , ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY , THE BRIGHAM AND WOMEN'S HOSPITAL, INC.
IPC分类号： G01N33/574
摘要： Compositions and methods relating to a panel of antigen biomarkers for the early detection of ovarian cancer. The compositions and methods encompass antigen biomarkers coupled to a substrate, with the biomarkers being selected from the group consisting of one or more of ICAM3, CTAG2, p53, STYXL1, PVR, POMC, NUDT11, TRIM39, UHMK1, KSR1, and NXF3.
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6.发明申请公开(公告)号：US20180201923A1
公开(公告)日：2018-07-19
申请号：US15904905
申请日：2018-02-26
发明人： Joshua LaBaer
CPC分类号： C12N15/1062 , G01N33/6845 , G01N33/6854 , G01N2458/10
摘要： Methods and compositions for multiplexed protein-protein interaction profiling (e.g., immunoprofiling), based on nucleic acid tagging of polypeptides (e.g., by RNA display) are described. In some embodiments the described compositions and methods utilize a library of prey polypeptide targets linked to prey RNAs encoding them, and a population of bait polypeptides, e.g., a mixture of antibodies, that bind to one or more of the prey polypeptide targets and are used to isolate and identify the bound prey polypeptide targets by amplification of their associated prey RNAs and sequencing of the corresponding cDNAs. In other embodiments the prey polypeptide targets are linked to DNA Bar Codes, which serve as unique identifiers of the tagged polypeptide.
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公开(公告)号：US20170363631A1
公开(公告)日：2017-12-21
申请号：US15534203
申请日：2015-12-09
发明人： Joshua LaBaer , Jie Wang , Ji Qiu , Garrick Wallstrom , Karen Anderson , Jin Park , Jonine Figueroa
IPC分类号： G01N33/574 , C12Q1/68 , C07K16/30 , C12N15/11
CPC分类号： G01N33/57415 , C07K16/3015 , C12N15/11 , C12Q1/6886 , C12Q2600/112 , C12Q2600/118 , C12Q2600/158 , G01N33/564
摘要： Cancer patients make antibodies to tumor-derived proteins that are potential biomarkers for early detection. Twenty-eight antigens have been identified as potential biomarkers for the early detection of basal-like breast cancer (Tables 1, 2). Also, a 13-AAb classifier has been developed that differentiate patients with BLBC from healthy controls with 33% sensitivity at 98% specificity (Table 3).
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公开(公告)号：US20240310369A1
公开(公告)日：2024-09-19
申请号：US18489398
申请日：2023-10-18
发明人： Joshua LaBaer , Bharath Takulapalli
IPC分类号： G01N33/543
CPC分类号： G01N33/54373 , G01N2440/00
摘要： Described herein are biosensor microarrays comprising detector polypeptide monolayers substantially free of contaminants. Also provided are methods for generation of such biosensor microarrays by capture of polypeptides by arrays comprising capture moieties and associated sensors.
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9.发明授权公开(公告)号：US12030909B2
公开(公告)日：2024-07-09
申请号：US16811573
申请日：2020-03-06
发明人： Joshua LaBaer
CPC分类号： C07H21/04 , B01D15/3819 , G01N33/6803 , G01N33/6854 , G01N2458/10
摘要： Provided herein are affinity reagents having affinity for particular target, each reagent having a unique DNA barcode, and methods for using the same to measure the abundance of targets in a sample. In particular, methods are provided in which unique barcodes linked to affinity reagents are contacted to a sample to bind antigens if present in said sample. In cases in which the affinity reagents are antibodies and the targets are antigens, antibodies that are bound to their target antigens can be separated from unbound antibodies and the DNA barcode associated with the affinity reagent is amplified, such as with a PCR reaction. In some cases, amplified barcode DNA is subjected to DNA sequencing as a measure of the levels of the target protein in the sample.
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10.发明申请公开(公告)号：US20210130391A1
公开(公告)日：2021-05-06
申请号：US16811573
申请日：2020-03-06
发明人： Joshua LaBaer
IPC分类号： C07H21/04
摘要： Provided herein are affinity reagents having affinity for particular target, each reagent having a unique DNA barcode, and methods for using the same to measure the abundance of targets in a sample. In particular, methods are provided in which unique barcodes linked to affinity reagents are contacted to a sample to bind antigens if present in said sample. In cases in which the affinity reagents are antibodies and the targets are antigens, antibodies that are bound to their target antigens can be separated from unbound antibodies and the DNA barcode associated with the affinity reagent is amplified, such as with a PCR reaction. In some cases, amplified barcode DNA is subjected to DNA sequencing as a measure of the levels of the target protein in the sample.
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