公开(公告)号：US20240294878A1

公开(公告)日：2024-09-05

申请号：US18578527

申请日：2022-07-13

Applicant: Arne JENSEN ,  Metin COLPAN

Inventor： Arne JENSEN ,  Metin COLPAN

IPC: C12N5/0775

CPC classification number: C12N5/0665 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/2306 ,  C12N2501/26 ,  C12N2502/1323 ,  C12N2533/52

Abstract: The invention relates to a method of producing mesenchymal stem cell derived exosomes comprising the steps of,
performing a method of producing a clonal mesenchymal stem cell line capable of producing exosomes (MSC-derived extracellular vesicles) comprising the steps of,
providing human induced pluripotent stem cells (hiPSCs),
generating therefrom an immortalized clonal cell line of mesenchymal stem cells (IMSCs),
characterizing the potential of the IMSCs to produce exosomes,
cultivating the desired IMSCs such that the IMSCs produce exosomes,
isolating the exosomes.
The invention relates to extracellular vesicles or exosomes, produced according to the invention.
The invention relates to the use of these EVs as a medicament and in a pharmaceutical composition.

公开(公告)号：US20250002860A1

公开(公告)日：2025-01-02

申请号：US18578612

申请日：2022-07-13

Applicant: Arne JENSEN ,  Metin COLPAN

Inventor： Arne JENSEN ,  Metin COLPAN

IPC: C12N5/0775 ,  A61K35/28 ,  C12N5/074

Abstract: The invention relates to a method of producing a clonal mesenchymal stem cell line capable of producing exosomes (MSC-derived extracellular vesicles) comprising the steps of, providing human induced pluripotent stem cells (hiPSCs), generating therefrom an immortalized clonal cell line of mesenchymal stem cells (IMSC), optionally characterizing the potential of the IMSCs to produce exosomes. The invention also relates to a stem cell line produced according to the invention. The invention relates to the cell line according to the invention, for use as a medicament. The invention relates to the cell line according to the invention for use in treating a disease that is amenable to treatment with stem cell therapy. The invention also relates to the use of a stem cell line according to the invention for producing EVs.

公开(公告)号：US20240293470A1

公开(公告)日：2024-09-05

申请号：US18578575

申请日：2022-07-13

Applicant: Arne JENSEN ,  Metin COLPAN

Inventor： Arne JENSEN ,  Metin COLPAN

IPC: A61K35/38 ,  C12N5/0775

CPC classification number: A61K35/38 ,  C12N5/0662 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/727 ,  C12N2506/1369

Abstract: The invention relates to mesenchymal stem cell derived exosomes produced by a method comprising the steps of,



performing a method of producing a clonal mesenchymal stem cell line capable of producing exosomes (MSC-derived extracellular vesicles) comprising the steps of, providing human induced pluripotent stem cells (hiPSCs),

generating therefrom an immortalized clonal cell line of mesenchymal stem cells (IMSCs),
characterizing the potential of the IMSCs to produce exosomes,


cultivating the desired IMSCs such that the IMSCs produce exosomes,
isolating the exosomes.

公开(公告)号：US20100159532A1

公开(公告)日：2010-06-24

申请号：US12640798

申请日：2009-12-17

Applicant: Metin COLPAN ,  Ralf Himmelreich ,  Thomas Rothmann

Inventor： Metin COLPAN ,  Ralf Himmelreich ,  Thomas Rothmann

IPC: C12P19/34 ,  C12M1/40

CPC classification number: B01L3/502 ,  B01L3/505 ,  B01L3/5082 ,  B01L7/52 ,  B01L2300/0832 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q2547/10

Abstract: The invention relates to a method and a device for performing a nucleic acid preparation and/or amplification.For the implementation of the method, a flexible tube is provided, which is always sealed below and hence at one end during the implementation of the sample preparation and/or amplification. In this tube, a biological sample is prepared and/or amplified. The use of only one tube is very economical. The tube is a disposable item which after a preparation or amplification can with its contents be disposed of environmentally correctly. Since moreover the tube has only to be filled with sample substances and reagents at a given time and manipulated, the method can also be simply and economically performed automatically.

Abstract translation: 本发明涉及一种用于进行核酸制备和/或扩增的方法和装置。 为了实现该方法，提供了柔性管，其在样品制备和/或扩增的实施期间总是被密封在下方，因此在其一端被密封。 在该管中，制备和/或扩增生物样品。 只使用一根管是非常经济的。 该管是一种一次性物品，其在制备或扩增后可以使其内容物在环境上被正确地处理。 此外，管子在给定的时间只需要填充样品物质和试剂并进行操作，该方法也可以简单和经济地自动执行。