公开(公告)号：US09556255B2

公开(公告)日：2017-01-31

申请号：US13893763

申请日：2013-05-14

申请人： Athenix Corporation

发明人： Kimberly S. Sampson ,  Daniel J. Tomso

IPC分类号： C12N15/82 ,  C07K14/325 ,  C07K16/12

CPC分类号： C07K14/325 ,  A01N37/18 ,  C07K16/1278 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:61-121 and 133-141, or the nucleotide sequence set forth in SEQ ID NO:1-60, 124-132, and 142-283, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：61-121和133-141所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-60,124所示的核苷酸序列 -132和142-283，以及其变体和片段。

公开(公告)号：US20130310543A1

公开(公告)日：2013-11-21

申请号：US13893801

申请日：2013-05-14

申请人： Athenix Corporation

发明人： Kimberly S. Sampson ,  Daniel J. Tomso ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell

IPC分类号： C07K16/12

CPC分类号： C07K14/325 ,  A01N37/18 ,  C07K16/1278 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:61-121 and 133-141, or the nucleotide sequence set forth in SEQ ID NO:1-60, 124-132, and 142-283, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：61-121和133-141所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-60,124所示的核苷酸序列 -132和142-283，以及其变体和片段。

公开(公告)号：US20130305412A1

公开(公告)日：2013-11-14

申请号：US13893763

申请日：2013-05-14

申请人： Athenix Corporation

发明人： Kimberly S. Sampson ,  Daniel J. Tomso

IPC分类号： C12N15/82

CPC分类号： C07K14/325 ,  A01N37/18 ,  C07K16/1278 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:61-121 and 133-141, or the nucleotide sequence set forth in SEQ ID NO:1-60, 124-132, and 142-283, as well as variants and fragments thereof.

公开(公告)号：US09909140B2

公开(公告)日：2018-03-06

申请号：US14733552

申请日：2015-06-08

申请人： Athenix Corporation

发明人： Kimberly S. Sampson ,  Daniel J. Tomso ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell

IPC分类号： C12N15/82 ,  C07K14/325 ,  C07K14/32 ,  A01N37/18 ,  C07K16/12

CPC分类号： C12N15/8286 ,  A01N37/18 ,  C07K14/32 ,  C07K14/325 ,  C07K16/1278 ,  Y02A40/162

摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.

公开(公告)号：US09238823B2

公开(公告)日：2016-01-19

申请号：US13779524

申请日：2013-02-27

申请人： Athenix Corporation

发明人： Kimberly S. Sampson ,  Daniel J. Tomso ,  Rong Guo

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C07K14/345 ,  C12N15/32 ,  A01N37/18 ,  A01N25/26 ,  C07K14/195 ,  C07K14/325

CPC分类号： C12N15/8286 ,  C07K14/195 ,  C07K14/325 ,  C07K14/345 ,  Y02A40/162

摘要： Compositions and methods for conferring insecticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for a Brevibacillus-derived delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, 7, or 10, or the nucleotide sequence set forth in SEQ ID NO:1, 3, 5, 6, 8, 9, 11, 12, 13, 14, or 15, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含短杆菌属来源的δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了具有编码SEQ ID NO：2,4,7或10所示的氨基酸序列的核苷酸序列的分离的核酸分子或者SEQ ID NO： 5，6，8，9，11，12，13，14或15，以及其变体和片段。

公开(公告)号：US20130303440A1

公开(公告)日：2013-11-14

申请号：US13893647

申请日：2013-05-14

申请人： Athenix Corporation

发明人： Kimberly S. Sampson ,  Daniel J. Tomso ,  Rong Guo

IPC分类号： C07K2/00

CPC分类号： C07K2/00 ,  C07K14/325 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:28-62, or the nucleotide sequence set forth in SEQ ID NO:1-27, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：28-62所示氨基酸序列的核苷酸序列或SEQ ID NO：1-27所示的核苷酸序列，以及变体和 片段。

公开(公告)号：US20130210712A1

公开(公告)日：2013-08-15

申请号：US13863776

申请日：2013-04-16

申请人： Athenix Corporation

发明人： Kimberly S. Sampson ,  Daniel J. Tomso

IPC分类号： C07K14/325 ,  A01N37/46

CPC分类号： C07K14/325 ,  A01N37/46 ,  C07K14/32 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:13-24 or the nucleotide sequence set forth in SEQ ID NO:1-12 and 25-44, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了包含编码SEQ ID NO：13-24所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-12和25-44所示的核苷酸序列的分离的核酸分子，以及 作为其变体和片段。

公开(公告)号：US20130104259A1

公开(公告)日：2013-04-25

申请号：US13675572

申请日：2012-11-13

申请人： ATHENIX CORPORATION

发明人： Kimberly S. Sampson ,  Daniel J. Tomso ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell

IPC分类号： C07K14/325 ,  A01N37/18 ,  C12N15/82 ,  C07K16/12

CPC分类号： C12N15/8286 ,  A01N37/18 ,  C07K14/32 ,  C07K14/325 ,  C07K16/1278 ,  Y02A40/162

摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.

公开(公告)号：US20170101447A1

公开(公告)日：2017-04-13

申请号：US15384581

申请日：2016-12-20

申请人： Athenix Corporation

发明人： Kimberly S. Sampson ,  Daniel J. Tomso

IPC分类号： C07K14/325 ,  A01N37/18 ,  C12N15/82

CPC分类号： C07K14/325 ,  A01N37/18 ,  C07K16/1278 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:61-121 and 133-141, or the nucleotide sequence set forth in SEQ ID NO:1-60, 124-132, and 142-283, as well as variants and fragments thereof.

公开(公告)号：US20150299274A1

公开(公告)日：2015-10-22

申请号：US14733552

申请日：2015-06-08

申请人： Athenix Corporation

发明人： Kimberly S. Sampson ,  Daniel J. Tomso ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell

IPC分类号： C07K14/325 ,  A01N37/18 ,  C12N15/82

CPC分类号： C12N15/8286 ,  A01N37/18 ,  C07K14/32 ,  C07K14/325 ,  C07K16/1278 ,  Y02A40/162

摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.

摘要翻译： 提供了赋予宿主细胞杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在宿主细胞中转化和表达。 组合物还包含转化的宿主细胞。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：4,5,6,13或14所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2,3,11或12，以及其变体和片段。