公开(公告)号：US20220356429A1

公开(公告)日：2022-11-10

申请号：US17743318

申请日：2022-05-12

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, JR. ,  Kristin G. BEAUMONT ,  Aathavan KARUNAKARAN ,  Natalie C. MARKS ,  Jason M. MCEWEN ,  Mark P. WHITE ,  J. Tanner NEVILL ,  Gang F. WANG ,  Andrew W. MCFARLAND ,  Daniele Malleo ,  Keith J. BREINLINGER ,  Xiao GUAN ,  Kevin T. CHAPMAN

IPC: C12M3/06 ,  C12M1/00

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

公开(公告)号：US20230023831A1

公开(公告)日：2023-01-26

申请号：US17930649

申请日：2022-09-08

Applicant: BERKELEY LIGHTS, INC

Inventor： Kristin G. BEAUMONT ,  Peter J. BEEMILLER ,  Volker L.S. KURZ ,  Gregory G. LAVIEU ,  Xiaohua WANG ,  Aathavan KARUNAKARAN

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/545 ,  G01N33/58

Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US20210071124A1

公开(公告)日：2021-03-11

申请号：US16920365

申请日：2020-07-02

Applicant: Berkeley Lights, Inc.

Inventor： Randall D. LOWE, JR. ,  Kristin G. BEAUMONT ,  Aathavan KARUNAKARAN ,  Natalie C. MARKS ,  Jason M. MCEWEN ,  Mark P. WHITE ,  J. Tanner NEVILL ,  Gang F. WANG ,  Andrew W. MCFARLAND ,  Daniele Malleo ,  Keith J. BREINLINGER ,  Xiao GUAN ,  Kevin T. CHAPMAN

IPC: C12M3/06 ,  C12M1/00

Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.