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1.发明授权Diluting reagent and method compelling time-independent consistency in MCV assay 有权稀释试剂和方法在MCV测定中引人注目的时间依赖性一致性公开(公告)号：US06225124B1
公开(公告)日：2001-05-01
申请号：US09323689
申请日：1999-06-02
申请人： Berend Houwen , Kinya Uchihashi , Yukio Hamaguchi , Rolf Mast
发明人： Berend Houwen , Kinya Uchihashi , Yukio Hamaguchi , Rolf Mast
IPC分类号： G01N3348
CPC分类号： G01N33/5094 , G01N15/12 , Y10T436/10 , Y10T436/101666 , Y10T436/108331 , Y10T436/2525 , Y10T436/25625
摘要： Aqueous blood-sample diluting reagent and method of its use for compelling a morphological change in a blood sample to yield an MCV value assayed at elapsed time after the sample is drawn to be consistent within a diagnostically acceptable range with the original, immediate post-drawing MCV value. Selection of a small amount of a predetermined surfactant added within a limited range of concentration, and of a salt for adjusting osmotic pressure of the sample is thereby determined. The blood sample is treated with an anti-coagulant agent immediately post-drawing, and for assay in a particle analyzer at post-drawing elapsed time is diluted with the reagent solution. The reagent has an osmotic pressure (&pgr;) of approximately 150-400 mOsm/kg and a pH of 6.0-8.5. The surfactant is present in a 0.0005% to 0.5% concentration and has a hydrophile-lipophile balance (HLB) of 10-20.
摘要翻译： 水性血液样品稀释试剂及其用于强制血液样品中形态学变化的方法，以产生在样品抽出后经过时间测定的MCV值，使其在诊断上可接受的范围内与原始的立即后绘图 MCV值。 由此确定少量在有限浓度范围内加入的预定表面活性剂和用于调节样品渗透压的盐的选择。 血液样品立即在拉伸后用抗凝剂处理，并且在抽出后经过时间的粒子分析仪中用试剂溶液稀释。 试剂的渗透压（pI）为约150-400mOsm / kg，pH为6.0-8.5。 表面活性剂的浓度为0.0005％至0.5％，亲水亲油平衡（HLB）为10-20。
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公开(公告)号：US06911313B2
公开(公告)日：2005-06-28
申请号：US09058323
申请日：1998-04-09
IPC分类号： G01N33/50 , G01N33/569 , G01N33/80 , G01N33/53 , G01N21/76
CPC分类号： G01N33/5094 , G01N15/1459 , G01N33/56972 , G01N33/80 , G01N2015/1006 , G01N2015/1402 , Y10T436/107497 , Y10T436/108331 , Y10T436/13 , Y10T436/25125 , Y10T436/2525 , Y10T436/25375
摘要： A method for discriminating and counting erythroblasts comprises the steps of: (i) staining leukocytes in a hematologic sample by adding a fluorescent labeled antibody capable of binding specifically with leukocytes to the hematologic sample; (ii) raising the permeability only of cell membranes of erythroblasts in the hematologic sample to a nucleotide fluorescent dye which does not permeate a cell membrane usually, the nucleotide fluorescent dye having a fluorescent spectrum capable of being distinguished from that of a fluorescent labeling compound of the fluorescent labeled antibody in step (i); (iii) staining nuclei of the erythroblasts in the hematologic sample with the nucleotide fluorescent dye; (iv) subjecting the hematologic sample to flowcytometry to detect at least two fluorescent signals from each cell; and (v) discriminating and counting the erythroblasts from difference in intensity between the at least two fluorescent signals.
摘要翻译： 用于鉴别和计数红细胞的方法包括以下步骤：（i）通过将能够与白细胞特异性结合的荧光标记抗体加入到血液样品中来染色血液学样品中的白细胞; （ii）将血液学样品中的红细胞的细胞膜的渗透性提高至不透过细胞膜的核苷酸荧光染料，所述核苷酸荧光染料具有与荧光标记化合物的荧光标记化合物不同的荧光光谱 步骤（i）中的荧光标记抗体; （iii）用核苷酸荧光染料染色血液样品中的红细胞核; （iv）使血液样品经流式细胞术检测来自每个细胞的至少两个荧光信号; 和（v）鉴别和计数红细胞与至少两个荧光信号之间的强度差异。
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公开(公告)号：US06900023B1
公开(公告)日：2005-05-31
申请号：US09388899
申请日：1999-09-02
IPC分类号： G01N33/49 , G01N15/14 , G01N33/50 , G01N33/53 , G01N33/536 , G01N33/569
CPC分类号： G01N33/56972 , G01N33/5094 , G01N2333/70546 , Y10S435/962 , Y10S436/80 , Y10S436/824 , Y10S436/825 , Y10T436/101666 , Y10T436/107497 , Y10T436/13 , Y10T436/25 , Y10T436/25125
摘要： A method for classifying and counting leukocytes comprises the steps of: (1) adding to a hematological sample the following fluorescence-labeled antibodies labeled with fluorescent dyes which emit fluorescences distinguishable from each other; (a) a first fluorescence-labeled antibody (1st antibody) which bonds specifically to leukocytes, (b) a second fluorescence-labeled antibody (2nd antibody) which bonds to at least one kind of neutrophilic cells, and (c) a third fluorescence-labeled antibody (3rd antibody) which bonds to at least one kind of immature granulocytic cells, in order to stain leukocytic cells in the sample, and removing erythrocytes from the sample; (2) analyzing the resulting sample using a flow cytometer to measure at least one scattered light signal and three separate fluorescence signals; (3) defining a group of granulocytic cells on the basis of intensity of the scattered light and intensity of fluorescence from the 1st antibody; (4) defining neutrophilic cells in the defined group of granulocylic cells on the basis of the intensity of the fluorescence from the 1st antibody and intensity of fluorescence from the 2nd or 3rd antibody; (5) classifying the defined group of the neutrophilic cells into groups of neutrophilic cells different in degree of maturity on the basis of the intensity of the fluorescence from the 2nd antibody and the intensity of the fluorescence from the 3rd antibody, and counting the number of cells in each of the groups.
摘要翻译： 用于白细胞分类和计数的方法包括以下步骤：（1）向血液样品中加入以荧光染料标记的荧光标记的抗体，所述荧光染料发射荧光彼此区分; （a）与白细胞特异性结合的第一荧光标记抗体（第一抗体），（b）与至少一种嗜中性粒细胞结合的第二荧光标记抗体（第二抗体），和（c）第三荧光 标记的抗体（第3抗体），其与至少一种未成熟的粒细胞细胞结合，以染色样品中的白细胞，并从样品中除去红细胞; （2）使用流式细胞仪分析所得样品以测量至少一个散射光信号和三个分离的荧光信号; （3）基于散射光的强度和第一抗体的荧光强度来定义一组粒细胞; （4）基于来自第一抗体的荧光强度和来自第二或第三抗体的荧光强度来定义所定义的颗粒细胞组中的嗜中性细胞; （5）基于来自第二抗体的荧光强度和来自第三抗体的荧光强度，将所定义的嗜中性粒细胞群分类成不同程度的嗜中性粒细胞组，并计数第 每个组中的细胞。
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公开(公告)号：USRE39006E1
公开(公告)日：2006-03-07
申请号：US10704463
申请日：2003-11-07
申请人： Berend Houwen , Yukio Tsujino , Takashi Morikawa , Yoshiro Ikeuchi , Yukio Hamaguchi , Fu-sheng Wang , Kojiro Hirai
发明人： Berend Houwen , Yukio Tsujino , Takashi Morikawa , Yoshiro Ikeuchi , Yukio Hamaguchi , Fu-sheng Wang , Kojiro Hirai
IPC分类号： C12Q1/04
CPC分类号： G01N33/5094
摘要： Improved method for detecting and/or counting the appearance of hematopoietic progenitor cells which comprises the steps of mixing a blood sample with a reagent capable of detecting immature cells without employing any immunological techniques, obtaining cell information about the thus treated sample using a particle analyzer and constructing a cell distribution profile, delineating a portion of the profile as a zone in which at least one subclass of hematopoietic progenitor cells appear, and counting the cells within the zone. The method is capable of detecting the appearance of hematopoietic progenitor cells in a simple and positive manner.
摘要翻译： 用于检测和/或计数造血祖细胞外观的改进方法，其包括以下步骤：将血液样品与能够检测未成熟细胞的试剂混合而不使用任何免疫技术，使用粒子分析仪获得关于如此处理的样品的细胞信息; 构建细胞分布轮廓，将轮廓的一部分描绘为其中存在至少一个造血祖细胞亚类的区域，并对该区域内的细胞进行计数。 该方法能够以简单和积极的方式检测造血祖细胞的外观。
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公开(公告)号：US5830701A
公开(公告)日：1998-11-03
申请号：US829239
申请日：1997-03-28
IPC分类号： G01N33/48 , C12N5/08 , G01N15/00 , G01N15/14 , G01N33/49 , G01N33/50 , C12Q1/04 , C12Q1/06 , G01N21/76
CPC分类号： G01N33/5094
摘要： Improved method for detecting and/or counting the appearance of hematopoietic progenitor cells which comprises the steps of mixing a blood sample with a reagent capable of detecting immature cells without employing any immunological techniques, obtaining cell information about the thus treated sample using a particle analyzer and constructing a cell distribution profile, delineating a portion of the profile as a zone in which at least one subclass of hematopoietic progenitor cells appear, and counting the cells within the zone. The method is capable of detecting the appearance of hematopoietic progenitor cells in a simple and positive manner.
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6.发明授权
公开(公告)号：US5968832A
公开(公告)日：1999-10-19
申请号：US57250
申请日：1998-04-08
CPC分类号： G01N33/5094 , G01N33/721 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25 , Y10T436/25125 , Y10T436/2525
摘要： A reagent for measurement of leukocytes and hemoglobin concentration in the blood includes a cationic surfactant in an amount sufficient to lyse erythrocytes and denature hemoglobin, at least one of the following hemoglobin stabilizers:(a) sulfosalicylic acid, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin,(b) 0.2 to 10.0 g/L of a water-soluble chelating agent having a nitrogen atom and a carboxyl group, and(c) piperazine, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, anda buffer for maintaining pH at 4 to 6.
摘要翻译： 白血球的测定用试剂和血液中的血红蛋白浓度包括阳离子表面活性剂，其量足以溶解红细胞，变性血红蛋白，以下血红蛋白稳定剂中的至少一种：（a）磺基水杨酸或其盐，有效量 用于促进血红蛋白转化成高铁血红蛋白，（b）0.2〜10.0g / L的具有氮原子和羧基的水溶性螯合剂，和（c）哌嗪或其盐，其量有效促进 将血红蛋白转化成高铁血红蛋白，以及将pH保持在4-6的缓冲液。
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7.再颁专利公开(公告)号：USRE38131E1
公开(公告)日：2003-06-03
申请号：US09977768
申请日：2001-10-15
IPC分类号： G01N3100
CPC分类号： G01N33/5094 , G01N33/721 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25 , Y10T436/25125 , Y10T436/2525
摘要： A reagent for measurement of leukocytes and hemoglobin concentration in the blood includes a cationic surfactant in an amount sufficient to lyse erythrocytes and denature hemoglobin, at least one of the following hemoglobin stabilizers: (a) sulfosalicylic acid, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, (b) 0.2 to 10.0 g/L of a water-soluble chelating agent having a nitrogen atom and a carboxyl group, and (c) piperazine, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, and a buffer for maintaining pH at 4 to 6.
摘要翻译： 白血球的测定用试剂和血液中的血红蛋白浓度包括阳离子表面活性剂，其量足以溶解红细胞，变性血红蛋白，以下血红蛋白稳定剂中的至少一种：（a）磺基水杨酸或其盐，有效量 用于促进血红蛋白转化成高铁血红蛋白，（b）0.2〜10.0g / L的具有氮原子和羧基的水溶性螯合剂，和（c）哌嗪或其盐，其量有效促进 将血红蛋白转化为高铁血红蛋白，以及将pH保持在4至6的缓冲液。
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公开(公告)号：US5651098A
公开(公告)日：1997-07-22
申请号：US319789
申请日：1994-10-07
申请人： Haruki Inoue , Mayumi Mizutani , Hideo Yoshida , Hitoshi Onizawa , Kenichi Nakamura , Yukio Hamaguchi , Masami Shiozawa
发明人： Haruki Inoue , Mayumi Mizutani , Hideo Yoshida , Hitoshi Onizawa , Kenichi Nakamura , Yukio Hamaguchi , Masami Shiozawa
CPC分类号： G06Q10/04 , Y10S706/922
摘要： A system for making an optimum plan for a given problem at high speed. The system sets a planning problem, etc., prepares an objective function and finalizes a plan for minimizing or maximizing the objective function value, and then stores necessary variables. The system prepares as many parent plans of a first generation as a given number (population), calculates objective function values, sorts plans in descending or ascending order of the objective function values of the plans, when selection numbers are assigned to plans in order of a ratio of the objective function value of each plan to the total of the objective function values of the plans, selects parent plans by using numbers indicated by as many constants as the population determined for each generation as the selection numbers, and replaces two elements, arranged at order positions specified by random numbers, with each other for each of the selected parent plans, to prepare child plans.
摘要翻译： 用于为给定问题高速制定最佳计划的系统。 系统设置规划问题等，准备目标函数，并确定最小化或最大化目标函数值的计划，然后存储必要的变量。 该系统准备与第一代作为给定数量（人口）一样多的父母计划，计算目标函数值，按照计划的目标函数值按降序或升序对计划进行排序，当选择编号按照 每个计划的目标函数值与计划的目标函数值的总和的比率，通过使用由与为每一代确定的群体一样多的常数指示的数字作为选择数来选择父计划，并且替换两个元素， 按照随机数指定的订单位置进行排列，对于每个选定的母计划，彼此分配，以制定子计划。
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公开(公告)号：US5389549A
公开(公告)日：1995-02-14
申请号：US964347
申请日：1992-10-21
申请人： Yukio Hamaguchi , Kenji Ito , Yukio Tsujino , Kazuhiro Moriyama , Ikuya Takenaka , Takashi Morikawa , Hitomi Ohmi
发明人： Yukio Hamaguchi , Kenji Ito , Yukio Tsujino , Kazuhiro Moriyama , Ikuya Takenaka , Takashi Morikawa , Hitomi Ohmi
CPC分类号： G01N33/5094 , G01N2015/1488 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： Reagents for enabling leukocytes to be classified and counted lyse erythrocytes and act on leukocytes to stabilize them are disclosed. The reagents contain polyoxyethylene-based surfactants, and may contain hyperosmotic or hypoosmotic agents and solubilizing agents. One embodiment of the invention contains surfactants selected from the anionic and nonionic polyoxyethylene-based surfactants having 18-30 repeating oxyethylene units per molecule. A second embodiment of the invention contains nonionic polyoxyethylene surfactants only, having 20-100 repeating oxyethylene units per molecule, does not employ either saponin or quaternary ammonium compounds, and provides for adjustment of the pH of the resulting solutions. The invention allows leukocytes to be classified into as many as five types, each type to be quantified, and enables the detection of abnormal cells, when used in conjunction with particulate analysis flow cytometers employing the RF method and the DC method.
摘要翻译： 公开了使白细胞分类和计数裂解红细胞并作用于白细胞以使其稳定的试剂。 试剂含有聚氧乙烯基表面活性剂，并且可能含有高渗或低渗透剂和增溶剂。 本发明的一个实施方案包含选自阴离子和非离子聚氧乙烯基表面活性剂的表面活性剂，每分子具有18-30个重复氧乙烯单元。 本发明的第二个实施方案仅含有非离子聚氧乙烯表面活性剂，每分子具有20-100个重复氧化乙烯单元，不使用皂苷或季铵化合物，并且用于调节所得溶液的pH。 本发明允许将白细胞分为多种五种类型，每种类型被定量，并且当与使用RF方法和DC方法的颗粒分析流式细胞仪结合使用时，能够检测异常细胞。
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10.发明授权
公开(公告)号：US5116539A
公开(公告)日：1992-05-26
申请号：US266860
申请日：1988-11-03
申请人： Yukio Hamaguchi , Yukio Tsujino
发明人： Yukio Hamaguchi , Yukio Tsujino
CPC分类号： G01N33/721 , G01N33/5094 , Y10S516/01 , Y10T436/101666
摘要： A reagent for and method of measuring leukocytes or hemoglobin or both in blood using a reagent which is water-soluble mixture containing:(a) a polyoxyethylene-based nonionic surfactant represented by the formula:R.sub.1 --R.sub.2 (CH.sub.2 CH.sub.2 O).sub.n --H where R.sub.1 is an alkyl, alkenyl or alkynyl group having 6 to 24 carbon atoms, R.sub.2 is --O--, ##STR1## or --COO--, and n is an integer of 6 to 50, and (b) a buffer to adjust the pH of the solution within the range of 3-11 is disclosed. Measurements of hemoglobin in blood samples are of extreme importance to clinical diagnosis of such diseases as leukemia and anemia. Measurements of eosinophils, which are in the category of leukocytes, are important to the diagnosis of allergic conditions.
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