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公开(公告)号：US5830701A
公开(公告)日：1998-11-03
申请号：US829239
申请日：1997-03-28
IPC分类号： G01N33/48 , C12N5/08 , G01N15/00 , G01N15/14 , G01N33/49 , G01N33/50 , C12Q1/04 , C12Q1/06 , G01N21/76
CPC分类号： G01N33/5094
摘要： Improved method for detecting and/or counting the appearance of hematopoietic progenitor cells which comprises the steps of mixing a blood sample with a reagent capable of detecting immature cells without employing any immunological techniques, obtaining cell information about the thus treated sample using a particle analyzer and constructing a cell distribution profile, delineating a portion of the profile as a zone in which at least one subclass of hematopoietic progenitor cells appear, and counting the cells within the zone. The method is capable of detecting the appearance of hematopoietic progenitor cells in a simple and positive manner.
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公开(公告)号：USRE39006E1
公开(公告)日：2006-03-07
申请号：US10704463
申请日：2003-11-07
申请人： Berend Houwen , Yukio Tsujino , Takashi Morikawa , Yoshiro Ikeuchi , Yukio Hamaguchi , Fu-sheng Wang , Kojiro Hirai
发明人： Berend Houwen , Yukio Tsujino , Takashi Morikawa , Yoshiro Ikeuchi , Yukio Hamaguchi , Fu-sheng Wang , Kojiro Hirai
IPC分类号： C12Q1/04
CPC分类号： G01N33/5094
摘要： Improved method for detecting and/or counting the appearance of hematopoietic progenitor cells which comprises the steps of mixing a blood sample with a reagent capable of detecting immature cells without employing any immunological techniques, obtaining cell information about the thus treated sample using a particle analyzer and constructing a cell distribution profile, delineating a portion of the profile as a zone in which at least one subclass of hematopoietic progenitor cells appear, and counting the cells within the zone. The method is capable of detecting the appearance of hematopoietic progenitor cells in a simple and positive manner.
摘要翻译： 用于检测和/或计数造血祖细胞外观的改进方法，其包括以下步骤：将血液样品与能够检测未成熟细胞的试剂混合而不使用任何免疫技术，使用粒子分析仪获得关于如此处理的样品的细胞信息; 构建细胞分布轮廓，将轮廓的一部分描绘为其中存在至少一个造血祖细胞亚类的区域，并对该区域内的细胞进行计数。 该方法能够以简单和积极的方式检测造血祖细胞的外观。
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3.再颁专利公开(公告)号：USRE38131E1
公开(公告)日：2003-06-03
申请号：US09977768
申请日：2001-10-15
IPC分类号： G01N3100
CPC分类号： G01N33/5094 , G01N33/721 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25 , Y10T436/25125 , Y10T436/2525
摘要： A reagent for measurement of leukocytes and hemoglobin concentration in the blood includes a cationic surfactant in an amount sufficient to lyse erythrocytes and denature hemoglobin, at least one of the following hemoglobin stabilizers: (a) sulfosalicylic acid, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, (b) 0.2 to 10.0 g/L of a water-soluble chelating agent having a nitrogen atom and a carboxyl group, and (c) piperazine, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, and a buffer for maintaining pH at 4 to 6.
摘要翻译： 白血球的测定用试剂和血液中的血红蛋白浓度包括阳离子表面活性剂，其量足以溶解红细胞，变性血红蛋白，以下血红蛋白稳定剂中的至少一种：（a）磺基水杨酸或其盐，有效量 用于促进血红蛋白转化成高铁血红蛋白，（b）0.2〜10.0g / L的具有氮原子和羧基的水溶性螯合剂，和（c）哌嗪或其盐，其量有效促进 将血红蛋白转化为高铁血红蛋白，以及将pH保持在4至6的缓冲液。
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4.发明授权
公开(公告)号：US5968832A
公开(公告)日：1999-10-19
申请号：US57250
申请日：1998-04-08
CPC分类号： G01N33/5094 , G01N33/721 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331 , Y10T436/25 , Y10T436/25125 , Y10T436/2525
摘要： A reagent for measurement of leukocytes and hemoglobin concentration in the blood includes a cationic surfactant in an amount sufficient to lyse erythrocytes and denature hemoglobin, at least one of the following hemoglobin stabilizers:(a) sulfosalicylic acid, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin,(b) 0.2 to 10.0 g/L of a water-soluble chelating agent having a nitrogen atom and a carboxyl group, and(c) piperazine, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, anda buffer for maintaining pH at 4 to 6.
摘要翻译： 白血球的测定用试剂和血液中的血红蛋白浓度包括阳离子表面活性剂，其量足以溶解红细胞，变性血红蛋白，以下血红蛋白稳定剂中的至少一种：（a）磺基水杨酸或其盐，有效量 用于促进血红蛋白转化成高铁血红蛋白，（b）0.2〜10.0g / L的具有氮原子和羧基的水溶性螯合剂，和（c）哌嗪或其盐，其量有效促进 将血红蛋白转化成高铁血红蛋白，以及将pH保持在4-6的缓冲液。
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公开(公告)号：US06525807B1
公开(公告)日：2003-02-25
申请号：US09488538
申请日：2000-01-21
申请人： Takashi Morikawa , Hiroyuki Inoue , Kimiyo Kubo , Yoshiro Ikeuchi
发明人： Takashi Morikawa , Hiroyuki Inoue , Kimiyo Kubo , Yoshiro Ikeuchi
IPC分类号： G01N2100
CPC分类号： G01N21/64 , G01N15/1456 , G01N21/47 , G01N2015/0073 , G01N2015/008 , G01N2015/0084 , G01N2015/0088 , G01N2015/1037 , G01N2015/1062 , G01N2015/1477 , G01N2015/1486
摘要： A particle analyzing apparatus outputs a measurement result with high reliability with respect to a measurement item. The particle analyzing apparatus includes a measuring part, a judgement processing part, and an output part. The measuring part includes a fluid part, a control part, a first measuring part, a second measuring part, a hystogram preparing part, and a scattergram preparing part. The fluid part sucks up a blood sample in a test tube by a pipette. The control part controls the fluid part, the first measuring part, and the second measuring part. Measurement data measured by the first measuring part and the second measuring part are sent to the judgement processing part, and after judgement is made in the judgement processing part, a measurement result is outputted by the output part. The first measuring part makes measurement on the basis of an impedance system, and the second measuring part makes measurement on the basis of a flow cytometory system.
摘要翻译： 粒子分析装置相对于测量项目输出具有高可靠性的测量结果。 粒子分析装置包括测量部分，判断处理部分和输出部分。 测量部分包括流体部分，控制部分，第一测量部分，第二测量部分，气瓶图准备部分和散点图准备部分。 流体部分通过移液管吸取试管中的血样。 控制部分控制流体部分，第一测量部分和第二测量部分。 由第一测量部分和第二测量部分测量的测量数据被发送到判断处理部分，并且在判断处理部分进行判断之后，由输出部分输出测量结果。 第一测量部件基于阻抗系统进行测量，第二测量部件基于流式细胞仪系统进行测量。
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公开(公告)号：US20090076736A1
公开(公告)日：2009-03-19
申请号：US12204736
申请日：2008-09-04
申请人： Yoshiro Ikeuchi , Shinichiro Oguni , Daigo Fukuma , Masanori Imazu , Jo Linssen
发明人： Yoshiro Ikeuchi , Shinichiro Oguni , Daigo Fukuma , Masanori Imazu , Jo Linssen
CPC分类号： G01N15/1459 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10T436/101666
摘要： A sample analyzer including a detection unit for irradiating a biological sample with light and obtaining optical information; a cell classification processor for classifying cells contained in the biological sample into cell groups based on the optical information; a scattered light information obtaining processor for obtaining scattered light information relating to a cell included in a predetermined cell group; a calculation processor for calculating a component value corresponding to an amount of component contained in cell included in the predetermined cell group based on the scattered light information; and an output device for outputting the component value calculated by the calculation means is disclosed. A computer program product is also disclosed.
摘要翻译： 一种样本分析器，包括用于用光照射生物样品并获得光学信息的检测单元; 细胞分类处理器，用于基于所述光学信息将所述生物样品中包含的细胞分类成细胞组; 散射光信息获取处理器，用于获得与包括在预定单元组中的单元有关的散射光信息; 计算处理器，用于基于散射光信息计算与包含在所述预定单元组中的单元中包含的分量的量相对应的分量值; 并且公开了一种用于输出由计算装置计算出的分量值的输出装置。 还公开了一种计算机程序产品。
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公开(公告)号：US07450223B2
公开(公告)日：2008-11-11
申请号：US11844522
申请日：2007-08-24
申请人： Yoshiro Ikeuchi , Shinichiro Oguni , Daigo Fukuma , Masanori Imazu , Jo Lissen
发明人： Yoshiro Ikeuchi , Shinichiro Oguni , Daigo Fukuma , Masanori Imazu , Jo Lissen
IPC分类号： G01N33/48
CPC分类号： G01N15/1459 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10T436/101666
摘要： A sample analyzer including a detection unit for irradiating a biological sample with light and obtaining optical information; a cell classification processor for classifying cells contained in the biological sample into cell groups based on the optical information; a scattered light information obtaining processor for obtaining scattered light information relating to a cell included in a predetermined cell group; a calculation processor for calculating a component value corresponding to an amount of component contained in cell included in the predetermined cell group based on the scattered light information; and an output device for outputting the component value calculated by the calculation means is disclosed. A computer program product is also disclosed.
摘要翻译： 一种样本分析器，包括用于用光照射生物样品并获得光学信息的检测单元; 细胞分类处理器，用于基于所述光学信息将所述生物样品中包含的细胞分类成细胞组; 散射光信息获取处理器，用于获得与包括在预定单元组中的单元有关的散射光信息; 计算处理器，用于基于散射光信息计算与包含在所述预定单元组中的单元中包含的分量的量相对应的分量值; 并且公开了一种用于输出由计算装置计算出的分量值的输出装置。 还公开了一种计算机程序产品。
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8.发明授权Method of staining, detection and counting bacteria, and a diluent for bacterial stain 有权染色，检测和计数细菌的方法，以及用于细菌染色的稀释剂公开(公告)号：US07309581B2
公开(公告)日：2007-12-18
申请号：US10005753
申请日：2001-10-29
CPC分类号： G01N1/30 , C12Q1/04 , G01N2001/302 , Y10T436/108331
摘要： A method of staining bacteria comprises: working a polymethine dye on a sample in the presence of a substance capable of reducing nitrite ions to stain bacteria in the sample. A method of detecting bacteria comprises the following steps of: (1) working a polymethine dye on a sample by a method as described above to stain bacteria in the sample, (2) introducing the thus treated sample into a detecting part of a flow cytometer and irradiating cells of the stained bacteria one by one with light to measure scattered light and fluorescent light emitted from each of the cells; and (3) discriminating the bacteria from other components in accordance with an intensity of a scattered light signal and an intensity of a fluorescent light signal or a pulse width reflecting the length of particles to count the bacteria.
摘要翻译： 染色细菌的方法包括：在能够还原亚硝酸根离子的物质存在下在样品上加工聚甲炔染料以染色样品中的细菌。 检测细菌的方法包括以下步骤：（1）通过上述方法在样品上加工聚甲炔染料以染色样品中的细菌，（2）将如此处理的样品引入流式细胞仪的检测部分 用光照射染色细菌的细胞，测量从每个细胞发出的散射光和荧光; 和（3）根据散射光信号的强度和荧光信号的强度或反映粒子长度的脉冲宽度来区别细菌与其它成分，以计数细菌。
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公开(公告)号：US20060223138A1
公开(公告)日：2006-10-05
申请号：US11392651
申请日：2006-03-30
CPC分类号： G01N15/147 , G01N33/5002 , G01N33/5094 , G01N33/80 , G01N2015/0073
摘要： A method for counting megakaryocytes in a specimen is described. In the method, first, erythrocytes in the specimen are lysed and nucleic acid in the megakaryocytes is stained with a fluorescent dye, and thereby, a measurement sample is prepared. Next, the cells in the measurement sample are irradiated with excited light so that the forward scattered light, the side scattered light and the fluorescence, which are emitted from the cells, are detected. Megakaryocytes are identified on the basis of the detected forward scattered light, the fluorescence and the side scattered light. Then, the identified megakaryocytes are counted.
摘要翻译： 描述了一种用于计数样本中的巨核细胞的方法。 在该方法中，首先将样品中的红细胞裂解，并用荧光染料染色巨核细胞中的核酸，由此制备测定样品。 接下来，用激发光照射测量样品中的单元，从而检测从单元发射的前向散射光，侧向散射光和荧光。 在检测到的前向散射光，荧光和侧向散射光的基础上鉴定巨核细胞。 然后，计算鉴定的巨核细胞。
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公开(公告)号：US20050219527A1
公开(公告)日：2005-10-06
申请号：US11076048
申请日：2005-03-10
申请人： Yoshiro Ikeuchi , Shinichiro Oguni , Daigo Fukuma , Masanori Imazu , Jo Linssen
发明人： Yoshiro Ikeuchi , Shinichiro Oguni , Daigo Fukuma , Masanori Imazu , Jo Linssen
CPC分类号： G01N15/1459 , G01N2015/1006 , G01N2015/1402 , G01N2015/1477 , Y10T436/101666
摘要： A sample analyzer including a detection unit for irradiating a biological sample with light and obtaining optical information; a cell classification processor for classifying cells contained in the biological sample into cell groups based on the optical information; a scattered light information obtaining processor for obtaining scattered light information relating to a cell included in a predetermined cell group; a calculation processor for calculating a component value corresponding to an amount of component contained in cell included in the predetermined cell group based on the scattered light information; and an output device for outputting the component value calculated by the calculation means is disclosed. A computer program product is also disclosed.
摘要翻译： 一种样本分析器，包括用于用光照射生物样品并获得光学信息的检测单元; 细胞分类处理器，用于基于所述光学信息将所述生物样品中包含的细胞分类成细胞组; 散射光信息获取处理器，用于获得与包括在预定单元组中的单元有关的散射光信息; 计算处理器，用于基于散射光信息计算与包含在所述预定单元组中的单元中包含的分量的量相对应的分量值; 并且公开了一种用于输出由计算装置计算出的分量值的输出装置。 还公开了一种计算机程序产品。
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