公开(公告)号：US20230323433A1

公开(公告)日：2023-10-12

申请号：US18178927

申请日：2023-03-06

Applicant: Berkeley Lights, Inc.

Inventor： Matthew Asuka Kubit ,  Joshua David Mast ,  John Junyeon Kim ,  Alexander Gerald Olson ,  Preston Lock Ng ,  Arlvin Louis Ellefson ,  Shruthi Sreedhar Kubatur ,  Vincent Haw Tien Pai ,  Minha Park ,  Po-Yuan Tung ,  Jason C. Briggs ,  Patrick N. Ingram ,  Katrine Elise Dailey ,  Maryam Shansab ,  Jason M. McEwen ,  Adrienne T. Higa ,  Hongye Zhou ,  Zhen Hu ,  John A. Tenney

IPC: C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6804

CPC classification number: C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6844 ,  C12Q2600/16

Abstract: Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can encode proteins of interest, such as B cell receptor (BCR) heavy and light chain sequences. The expression constructs can be generated individually or in a paired/multiplexed manner, allowing rapid re-expression of individual proteins or protein complexes.

公开(公告)号：US20220401954A1

公开(公告)日：2022-12-22

申请号：US17660448

申请日：2022-04-25

Applicant: Berkeley Lights, Inc.

Inventor： Angel Navas Angeles ,  Johannes Paul Sluis ,  John A. Tenney ,  Yogesh Khemchandra Dhande ,  Patrick N. Ingram ,  Erin Chia-wei Hsi ,  Christopher C. Shing ,  John Junyeon Kim ,  Keith J. Breinlinger ,  Raziel Solomon Alon

IPC: B01L3/00 ,  B01L9/00 ,  G02B21/06 ,  G01N35/00 ,  G01N35/10

Abstract: Systems for operating a microfluidic device are described. The systems comprise a first surface configured to interface and operatively couple with a microfluidic device and a lid configured to retain the microfluidic device on the first surface. The lid comprises a first portion having a first fluid port configured to operatively couple with and flow fluidic medium into and/or out of a first fluid inlet/outlet of the microfluidic device and a second portion having a second fluid port configured to operatively couple with and flow fluidic medium into and/or out of a second fluid inlet/outlet of the microfluidic device. The second portion of the lid is separable from the first portion and movable between a closed position in which the second fluid port of the second portion of the cover is operatively coupled with the second fluid inlet/outlet of the microfluidic device and an open position in which a portion of the microfluidic device that contains the second fluid inlet/outlet is exposed. Other embodiments are described.