公开(公告)号：US20230323433A1

公开(公告)日：2023-10-12

申请号：US18178927

申请日：2023-03-06

申请人： Berkeley Lights, Inc.

发明人： Matthew Asuka Kubit ,  Joshua David Mast ,  John Junyeon Kim ,  Alexander Gerald Olson ,  Preston Lock Ng ,  Arlvin Louis Ellefson ,  Shruthi Sreedhar Kubatur ,  Vincent Haw Tien Pai ,  Minha Park ,  Po-Yuan Tung ,  Jason C. Briggs ,  Patrick N. Ingram ,  Katrine Elise Dailey ,  Maryam Shansab ,  Jason M. McEwen ,  Adrienne T. Higa ,  Hongye Zhou ,  Zhen Hu ,  John A. Tenney

IPC分类号： C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6804

CPC分类号： C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6844 ,  C12Q2600/16

摘要： Disclosed herein are methods for performing assays, including general functional assays, on a biological cell. Also disclosed herein are methods of barcoding the 5′ ends of RNA from a biological cell and methods of preparation of expression constructs from the barcoded RNA. The barcoded RNA can encode proteins of interest, such as B cell receptor (BCR) heavy and light chain sequences. The expression constructs can be generated individually or in a paired/multiplexed manner, allowing rapid re-expression of individual proteins or protein complexes.

公开(公告)号：US20200064337A1

公开(公告)日：2020-02-27

申请号：US16391063

申请日：2019-04-22

申请人： Berkeley Lights, Inc.

发明人： Minha Park ,  Jason C. Briggs ,  Jason M. McEwen ,  Ravi K. Ramenani ,  Hariharasudhan Chirra Dinakar ,  Kai W. Szeto ,  Adrienne T. Higa ,  Mark P. White ,  Randall D. Lowe, JR. ,  Xiaohua Wang ,  Kevin T. Chapman

IPC分类号： G01N33/50 ,  B01L3/00 ,  C12N5/0781 ,  G01N33/68 ,  C12Q1/6876

摘要： Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described.

公开(公告)号：US10712344B2

公开(公告)日：2020-07-14

申请号：US15406289

申请日：2017-01-13

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  George L. Fox ,  Peggy A. Radel ,  Mark P. White ,  Xiaohua Wang ,  Minha Park ,  Guido K. Stadler ,  Randall D. Lowe, Jr. ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Gang F. Wang

IPC分类号： B01L3/00 ,  G01N33/574 ,  C07K16/00 ,  G01N33/569 ,  C07K16/28 ,  G01N33/50 ,  C07K16/30 ,  C12Q1/6886 ,  G01N33/543

摘要： A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

公开(公告)号：US11971409B2

公开(公告)日：2024-04-30

申请号：US16892642

申请日：2020-06-04

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Mark P. White ,  Xiaohua Wang ,  Minha Park ,  Guido K. Stadler ,  Randall D. Lowe, Jr. ,  Xiao Guan Radstrom ,  Jason M. McEwen ,  Gang F. Wang ,  George L. Fox ,  Peggy A. Radel

IPC分类号： B01L3/00 ,  C07K16/00 ,  C07K16/28 ,  C07K16/30 ,  C12Q1/6886 ,  G01N33/50 ,  G01N33/543 ,  G01N33/569 ,  G01N33/574

CPC分类号： G01N33/574 ,  B01L3/502761 ,  C07K16/00 ,  C07K16/2887 ,  C07K16/30 ,  C12Q1/6886 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/54366 ,  G01N33/56972 ,  B01L2200/0647 ,  G01N2800/7028

摘要： A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

公开(公告)号：US20170276679A1

公开(公告)日：2017-09-28

申请号：US15406289

申请日：2017-01-13

申请人： Berkeley Lights, Inc.

发明人： Kevin T. Chapman ,  Mark P. White ,  Xiaohua Wang ,  Minha Park ,  Guido K. Stadler ,  Randall D. Lowe, Jr. ,  Xiao Guan ,  Jason M. McEwen ,  Gang Wang ,  George L. Fox ,  Peggy A. Radel

IPC分类号： G01N33/574 ,  C12Q1/68 ,  G01N33/543 ,  B01L3/00

摘要： A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.