公开(公告)号：US20200171501A1

公开(公告)日：2020-06-04

申请号：US16661310

申请日：2019-10-23

申请人： Berkeley Lights, Inc.

发明人： Jason M. McEwen ,  Magali Soumillon ,  Shao Ning Pei ,  Randall D. Lowe, Jr. ,  Samira A. Nedungadi ,  Volker L.S. Kurz ,  Jian Gong ,  Yara X. Mejia Gonzalez ,  Mckenzi S. Toh ,  Brian A. Rabkin ,  Jason C. Briggs ,  Darcy K. Kelly-Greene ,  James M. Porter, Jr.

IPC分类号： B01L3/00 ,  B01L7/00 ,  B03C5/00 ,  B03C5/02

摘要： Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided for processing biological cells, e.g., for use in nucleic acid library preparation and/or synthesis (including amplification). The devices include a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode. Methods of nucleic acid library preparation and/or synthesis can involve providing reagents to cells or nucleic acids by merging appropriate droplets on a droplet actuation surface within a water-immiscible organic liquid and can be performed in the presence of appropriate surfactants. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are systems for temperature control of the microfluidic device during nucleic acid library preparation and/or synthesis which can reduce temperature overshooting during heating and cooling steps.

公开(公告)号：US20200064337A1

公开(公告)日：2020-02-27

申请号：US16391063

申请日：2019-04-22

申请人： Berkeley Lights, Inc.

发明人： Minha Park ,  Jason C. Briggs ,  Jason M. McEwen ,  Ravi K. Ramenani ,  Hariharasudhan Chirra Dinakar ,  Kai W. Szeto ,  Adrienne T. Higa ,  Mark P. White ,  Randall D. Lowe, JR. ,  Xiaohua Wang ,  Kevin T. Chapman

IPC分类号： G01N33/50 ,  B01L3/00 ,  C12N5/0781 ,  G01N33/68 ,  C12Q1/6876

摘要： Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described.