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    Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
    4.
    发明授权
    Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production 失效
    表达金属蛋白酶水平降低的宿主细胞和使用宿主细胞在蛋白质生产中的方法

    公开(公告)号：US5861280A

    公开(公告)日：1999-01-19

    申请号：US894772

    申请日：1997-08-27

    申请人： Jan Lehmbeck

    发明人： Jan Lehmbeck

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N9/58 ,  C12N9/62 ,  C12N9/64 ,  C12N15/80 ,  C12P21/00 ,  C12P21/02

    CPC分类号： C12N9/6483 ,  C12N15/80 ,  C12N9/58 ,  C12N9/62 ,  C12Y304/23004

    摘要： The present invention relates to novel host cells and to methods of producing proteins. More specifically the invention relates to a host cell useful for the expression of heterologous proteins, which host cell has been genetically modified in order to express significantly reduced levels of a metalloprotease. Moreover the invention relates to a method of producing a heterologous protein, which method comprises cultivating the host cell in a suitable growth medium, followed by recovery of the desired protein.

    摘要翻译： PCT No.PCT / DK96 / 00111 Sec。 371日期1997年8月27日第 102（e）日期1997年8月27日PCT 1996年3月20日PCT公布。 公开号WO96 / 29391 日期1996年9月26日本发明涉及新型宿主细胞和生产蛋白质的方法。 更具体地，本发明涉及可用于表达异源蛋白质的宿主细胞，其宿主细胞已被遗传修饰以表达显着降低的金属蛋白酶水平。 此外，本发明涉及产生异源蛋白质的方法，该方法包括在合适的生长培养基中培养宿主细胞，然后回收所需的蛋白质。

    Use of Fungal Mutants for Expression of Antibodies
    5.
    发明申请
    Use of Fungal Mutants for Expression of Antibodies 有权
    使用真菌突变体表达抗体

    公开(公告)号：US20080248530A1

    公开(公告)日：2008-10-09

    申请号：US12065924

    申请日：2006-10-17

    申请人： Mogens Trier Hansen ,  Christian Isak Jorgensen ,  Jan Lehmbeck

    发明人： Mogens Trier Hansen ,  Christian Isak Jorgensen ,  Jan Lehmbeck

    IPC分类号： C12P21/00 ,  C12N1/14

    CPC分类号： C12N9/58 ,  C07K16/00 ,  C12N9/62 ,  C12N15/80 ,  C12P21/02

    摘要： The present invention relates to a filamentous fungal cell in which an endogenous alkaline protease activity, an endogenous neutral metalloprotease activity and an endogenous serine protease activity of the subtilisin type have been completely or partially inactivated. Particularly the endogenous alkaline protease activity, the endogenous neutral metalloprotease activity and the endogenous serine protease activity of the subtilisin type are encoded by the alp, npI and pepC genes respectively. The filamentous fungal cell is particularly suitable for production of heterologous proteins such as antibodies.

    摘要翻译： 本发明涉及丝状真菌细胞，其中内源性碱性蛋白酶活性，内源性中性金属蛋白酶活性和枯草杆菌蛋白酶类型的内源性丝氨酸蛋白酶活性已被完全或部分失活。 特别地，内源性碱性蛋白酶活性，内源性中性金属蛋白酶活性和枯草杆菌蛋白酶类型的内源性丝氨酸蛋白酶活性分别由alp，npI和pepC基因编码。 丝状真菌细胞特别适用于产生异源蛋白如抗体。

    Methods for producing polypeptides in aspergillus mutant cells
    6.
    发明授权
    Methods for producing polypeptides in aspergillus mutant cells 失效
    在曲霉突变体细胞中产生多肽的方法

    公开(公告)号：US06828137B2

    公开(公告)日：2004-12-07

    申请号：US10099704

    申请日：2002-03-15

    申请人： Bjorn Eggert Christensen ,  Henrik Mollgaard ,  Svend Kaasgaard ,  Jan Lehmbeck

    发明人： Bjorn Eggert Christensen ,  Henrik Mollgaard ,  Svend Kaasgaard ,  Jan Lehmbeck

    IPC分类号： C12N988

    CPC分类号： C12N9/1085 ,  C12N9/00 ,  C12N9/10 ,  C12N9/20 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12R1/69 ,  C12Y302/01008

    摘要： The present invention relates to methods for producing a polypeptide of interest by (a) cultivating a mutant of a parent Aspergillus cell, wherein (i) the mutant comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes responsible for the biosynthesis or secretion of at least one toxin, and (ii) the mutant produces less of the toxin than the parent Aspergillus cell when cultured under the same conditions; and (b) isolating the polypeptide from the culture medium. Also, mutants of Aspergillus cells are provided, as well as methods for obtaining the mutant cells.

    摘要翻译： 本发明涉及通过（a）培养母体曲霉细胞的突变体来产生感兴趣多肽的方法，其中（i）突变体包含编码多肽的第一核酸序列和包含修饰的第二核酸序列 负责至少一种毒素的生物合成或分泌的基因中的至少一个，和（ii）当在相同条件下培养时，突变体产生比母体曲霉细胞少的毒素; 和（b）从培养基中分离多肽。 此外，提供了曲霉菌细胞的突变体，以及获得突变细胞的方法。

    Host cells and methods of producing proteins
    7.
    发明授权
    Host cells and methods of producing proteins 有权
    宿主细胞和产生蛋白质的方法

    公开(公告)号：US06352841B1

    公开(公告)日：2002-03-05

    申请号：US09252509

    申请日：1999-02-18

    申请人： Jan Lehmbeck

    发明人： Jan Lehmbeck

    IPC分类号： C12P2102

    CPC分类号： C12N9/62 ,  C12N9/20 ,  C12N9/58 ,  C12N15/80

    摘要： The present invention relates to novel host cells and to methods of producing proteins. More specifically the invention relates to a host cell useful for the expression of heterologous proteins, in which the host cell has been genetically modified in order to express significantly reduced levels of a metalloprotease and an alkaline protease. Moreover the invention relates to a method of producing a heterologous protein, which method comprises cultivating the host cell in a suitable growth medium, followed by recovery of the desired protein.

    摘要翻译： 本发明涉及新型宿主细胞和生产蛋白质的方法。 更具体地，本发明涉及用于表达异源蛋白质的宿主细胞，其中宿主细胞已被遗传修饰以表达显着降低的金属蛋白酶和碱性蛋白酶的水平。 此外，本发明涉及产生异源蛋白质的方法，该方法包括在合适的生长培养基中培养宿主细胞，然后回收所需的蛋白质。

    FUNGAL PEPC INHIBITOR
    8.
    发明申请
    FUNGAL PEPC INHIBITOR 失效
    真菌PEPC抑制剂

    公开(公告)号：US20100273980A1

    公开(公告)日：2010-10-28

    申请号：US12742545

    申请日：2008-12-02

    申请人： Jan Lehmbeck ,  Hiroaki Udagawa

    发明人： Jan Lehmbeck ,  Hiroaki Udagawa

    IPC分类号： C07K14/38 ,  C12P21/02

    CPC分类号： C07K14/38 ,  C12N15/80

    摘要： The present invention relates to an isolated polypeptide having PepC inhibitory activity as well as to a method for producing a heterologous polypeptide of interest in an Aspergillus host cell comprising: (a) cultivating the Aspergillus host cell comprising a first and a second nucleic acid sequences under conditions conducive for the expression of the polypeptides encoded by the said first and second nucleic acid sequences, and wherein the first nucleic acid sequence encodes a heterologous polypeptide of interest and the second nucleic acid encodes the inhibitor polypeptide of the invention, and wherein the inhibitor polypeptide is expressed from a recombinant nucleic acid construct resulting in an increased level of the inhibitor polypeptide compared to an Aspergillus host cell not comprising the recombinant nucleic acid construct; and (b) recovering the heterologous polypeptide.

    摘要翻译： 本发明涉及具有PepC抑制活性的分离多肽以及在曲霉宿主细胞中产生感兴趣的异源多肽的方法，包括：（a）培养包含第一和第二核酸序列的曲霉属宿主细胞， 有利于由所述第一和第二核酸序列编码的多肽的表达的条件，并且其中所述第一核酸序列编码感兴趣的异源多肽，并且所述第二核酸编码本发明的抑制剂多肽，并且其中所述抑制剂多肽 由重组核酸构建体表达，与不包含重组核酸构建体的曲霉属宿主细胞相比，导致抑制剂多肽的水平增加; 和（b）回收异源多肽。

    Overexpression of the Chaperone BIP in a Heterokaryon
    9.
    发明申请
    Overexpression of the Chaperone BIP in a Heterokaryon 审中-公开
    分子伴侣BIP在异位核苷酸中的过表达

    公开(公告)号：US20100062491A1

    公开(公告)日：2010-03-11

    申请号：US12519480

    申请日：2008-01-03

    申请人： Jan Lehmbeck

    发明人： Jan Lehmbeck

    IPC分类号： C12P21/00

    CPC分类号： C07K16/00 ,  C12N15/80 ,  C12P21/02

    摘要： The present invention relates to a method for increasing the production yield of a secreted antibody or antibody fragment in a filamentous fungal host cell, comprising: recombinant expression of the antibody or antibody fragment and over-expressing a BiP chaperone protein.

    摘要翻译： 本发明涉及增加丝状真菌宿主细胞中分泌的抗体或抗体片段的产量的方法，其包括：重组表达所述抗体或抗体片段并过度表达BiP伴侣蛋白。

    Methods for producing polypeptides in Aspergillus mutant cells
    10.
    发明授权
    Methods for producing polypeptides in Aspergillus mutant cells 有权
    在曲霉突变体细胞中产生多肽的方法

    公开(公告)号：US07241614B2

    公开(公告)日：2007-07-10

    申请号：US10973973

    申请日：2004-10-26

    申请人： Bjorn Eggert Christensen ,  Henrik Mollgaard ,  Svend Kaasgaard ,  Jan Lehmbeck

    发明人： Bjorn Eggert Christensen ,  Henrik Mollgaard ,  Svend Kaasgaard ,  Jan Lehmbeck

    IPC分类号： C12N1/14 ,  C12P21/06 ,  C12P21/04

    CPC分类号： C12N9/1085 ,  C12N9/00 ,  C12N9/10 ,  C12N9/20 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/88 ,  C12N15/80 ,  C12P21/02 ,  C12R1/69 ,  C12Y302/01008

    摘要： The present invention relates to methods for producing a polypeptide of interest by (a) cultivating a mutant of a parent Aspergillus cell, wherein (i) the mutant comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes responsible for the biosynthesis or secretion of at least one toxin, and (ii) the mutant produces less of the toxin than the parent Aspergillus cell when cultured under the same conditions; and (b) isolating the polypeptide from the culture medium. Also, mutants of Aspergillus cells are provided, as well as methods for obtaining the mutant cells.

    摘要翻译： 本发明涉及通过（a）培养母体曲霉细胞的突变体来产生感兴趣多肽的方法，其中（i）突变体包含编码多肽的第一核酸序列和包含修饰的第二核酸序列 负责至少一种毒素的生物合成或分泌的基因中的至少一个，和（ii）当在相同条件下培养时，突变体产生比母体曲霉细胞少的毒素; 和（b）从培养基中分离多肽。 此外，提供了曲霉菌细胞的突变体，以及获得突变细胞的方法。