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公开(公告)号：US20120058222A1
公开(公告)日：2012-03-08
申请号：US13319426
申请日：2010-05-19
申请人： Bo Spange Sorensen , Inge Lise Povlsen , Rie Mejldal , Karsten Matthias Kragh , Anja Hemmingsen Kellett-Smith , Rene Mikkelsen , Rikke L. Bundgaard Jenner
发明人： Bo Spange Sorensen , Inge Lise Povlsen , Rie Mejldal , Karsten Matthias Kragh , Anja Hemmingsen Kellett-Smith , Rene Mikkelsen , Rikke L. Bundgaard Jenner
CPC分类号： C12N9/20 , A21D8/042 , C12N9/1029 , C12N9/2417
摘要： The present invention relates to the use of an amylase and a lipolytic enzyme in combination to improve the stackability of bread, methods of preparing dough and baked products having a combination of such enzymes, as well as bread having particular bread stackability profiles.
摘要翻译： 本发明涉及组合淀粉酶和脂肪分解酶以改善面包的可堆叠性的用途，制备面团的方法和具有这种酶的组合的烘焙产品，以及具有特定面包堆叠性的面包。
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公开(公告)号：US08753859B2
公开(公告)日：2014-06-17
申请号：US13287492
申请日：2011-11-02
申请人： Karsten Matthias Kragh , Anja Hemmingsen Kellet-Smith , René Mikkelsen , Rie Mejldal , Rikke L. Bundgaard Jenner
发明人： Karsten Matthias Kragh , Anja Hemmingsen Kellet-Smith , René Mikkelsen , Rie Mejldal , Rikke L. Bundgaard Jenner
CPC分类号： C12P19/14 , C12N9/2417
摘要： This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products.
摘要翻译： 本发明涉及多肽，更具体地涉及淀粉酶多肽和编码这些多肽的核酸及其用途。 作为生产食品或饲料产品的非麦芽糖外消旋酶。
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公开(公告)号：US20120190075A1
公开(公告)日：2012-07-26
申请号：US13287492
申请日：2011-11-02
申请人： Karsten Matthias Kragh , Anja Hemmingsen Kellet-Smith , René Mikkelsen , Rie Mejldal , Rikke L. Bundgaard Jenner
发明人： Karsten Matthias Kragh , Anja Hemmingsen Kellet-Smith , René Mikkelsen , Rie Mejldal , Rikke L. Bundgaard Jenner
CPC分类号： C12P19/14 , C12N9/2417
摘要： This invention relates to polypeptides, more specifically amylase polypeptides and nucleic acids encoding these, and their uses e.g. as non-maltogenic exoamylases in producing food or feed products.
摘要翻译： 本发明涉及多肽，更具体地涉及淀粉酶多肽和编码这些多肽的核酸及其用途。 作为生产食品或饲料产品的非麦芽糖外消旋酶。
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公开(公告)号：US20090202675A1
公开(公告)日：2009-08-13
申请号：US12339718
申请日：2008-12-19
申请人： Patrick Maria Franciscus Derkx , Anja Kellet-Smith Hemmingen , Rie Mejldal , Bo Spange Sorensen , Karsten Matthias Kragh
发明人： Patrick Maria Franciscus Derkx , Anja Kellet-Smith Hemmingen , Rie Mejldal , Bo Spange Sorensen , Karsten Matthias Kragh
IPC分类号： A21D2/26 , C12N9/28 , C12P19/00 , C12N15/11 , C12N15/00 , C12N1/21 , C12N1/19 , C12P21/02 , A23L1/105 , A23K1/165 , A21D13/00
CPC分类号： C12N9/2417 , C12N9/2411 , C12N9/2414
摘要： We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 307 to lysine (K) or arginine (R), with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide further comprises an amino acid substitution at position 70, preferably G70D. The amino acid at positions 272 and 303 of the sequence of the are preferably histidine (H) and glycine (G).
摘要翻译： 我们描述了可从具有非麦芽糖外切酶活性的亲本多肽衍生的PS4变体多肽，其中PS4变体多肽包含307位氨基酸取代赖氨酸（K）或精氨酸（R），参考位置编号 优选地，PS4变体多肽还包含70位氨基酸取代，优选G70D。 序列272和303位氨基酸优选为组氨酸（H）和甘氨酸（G）。
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5.发明申请Exo-Specific Amylase Polypeptides, Nucleic Acids Encoding Those Polypeptides And Uses Thereof 有权超特异性淀粉酶多肽，编码这些多肽的核酸及其用途公开(公告)号：US20080274531A1
公开(公告)日：2008-11-06
申请号：US12024324
申请日：2008-02-01
申请人： Casper Tune Berg , Kirsten Bojsen , Patrick M.F. Derkx , Carol Fioresi , Gijsbert Gerritse , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Charlotte Refdahl Thoudahl , Anja Hemmingsen Kellett-Smith
发明人： Casper Tune Berg , Kirsten Bojsen , Patrick M.F. Derkx , Carol Fioresi , Gijsbert Gerritse , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Charlotte Refdahl Thoudahl , Anja Hemmingsen Kellett-Smith
CPC分类号： C12N9/2417
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically the amylases of the current invention show an altered exospecifity.
摘要翻译： 本发明涉及淀粉酶多肽，以及编码多肽的核酸及其用途。 本发明的淀粉酶已被设计成具有更多的有益品质。 具体地说，本发明的淀粉酶表现出改变的特异性。
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6.发明授权Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof 有权特异性淀粉酶多肽，编码那些多肽的核酸及其用途公开(公告)号：US08809032B2
公开(公告)日：2014-08-19
申请号：US12024324
申请日：2008-02-01
申请人： Casper Tune Berg , Kirsten Bojsen , Patrick M. F. Derkx , Carol Fioresi , Gijsbert Gerritse , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Charlotte Refdahl Thoudahl , Anja Hemmingsen Kellett-Smith
发明人： Casper Tune Berg , Kirsten Bojsen , Patrick M. F. Derkx , Carol Fioresi , Gijsbert Gerritse , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Charlotte Refdahl Thoudahl , Anja Hemmingsen Kellett-Smith
CPC分类号： C12N9/2417
摘要： This invention relates to amylase polypeptides, and nucleic acids encoding the polypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically the amylases of the current invention show an altered exospecifity.
摘要翻译： 本发明涉及淀粉酶多肽，以及编码多肽的核酸及其用途。 本发明的淀粉酶已被设计成具有更多的有益品质。 具体地说，本发明的淀粉酶表现出改变的特异性。
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公开(公告)号：US20080227173A1
公开(公告)日：2008-09-18
申请号：US11534624
申请日：2006-09-22
申请人： Casper Tune BERG , Patrick Maria Franciscus Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Hemmingen Kellet-Smith , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Bo Spange Sorensen , Charlotte Refdahl Thoudahl
发明人： Casper Tune BERG , Patrick Maria Franciscus Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Hemmingen Kellet-Smith , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Bo Spange Sorensen , Charlotte Refdahl Thoudahl
IPC分类号： C12N9/28
CPC分类号： A21D8/042 , A23K20/147 , A23K50/30 , A23K50/75 , A23L33/18 , C12N9/2411 , C12N9/2414 , C12N9/2417 , C12N9/2425 , C12N9/2428
摘要： The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.
摘要翻译： 本发明描述了可衍生自具有选自以下的淀粉酶活性的亲本多肽的PS4变体多肽： 161,178,179,223,229,272,303,307,309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参照SEQ ID NO：1所示的假单胞菌属酵母胞外淀粉酶序列的位置编号，这种多肽作为食品或饲料添加剂的使用，以及编码这样的核酸。
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8.发明申请公开(公告)号：US20080292747A1
公开(公告)日：2008-11-27
申请号：US11970473
申请日：2008-01-07
申请人： Casper Tune Berg , Patrick Maria Franciscus Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Hemmingen Kellet-Smith , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Bo Spange Sorensen , Charlotte Refdahl Thoudahl
发明人： Casper Tune Berg , Patrick Maria Franciscus Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Hemmingen Kellet-Smith , Karsten Matthias Kragh , Wei Liu , Andrew Shaw , Bo Spange Sorensen , Charlotte Refdahl Thoudahl
CPC分类号： A21D8/042 , A23L33/18 , C12N9/2411 , C12N9/2414 , C12N9/2417 , C12N9/2425 , C12N9/2428
摘要： Described is a PS4 variant polypeptide derivable from a polypeptide having amylase activity selected from: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; referring to the numbering of a Pseudomonas saccharophilia exoamylase shown as SEQ ID NO: 1.
摘要翻译： 描述了可衍生自具有选自以下的淀粉酶活性的多肽的PS4变体多肽： 223,229,272,303,307,309和334; （b）包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; （c）包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和（d）包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 指的是SEQ ID NO：1所示的假单胞菌糖酵母外切酶的编号。
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公开(公告)号：US08178336B2
公开(公告)日：2012-05-15
申请号：US12339718
申请日：2008-12-19
申请人： Patrick Maria Franciscus Derkx , Anja Kellet-Smith Hemmingen , Rie Mejldal , Bo Spange Sørensen , Karsten Matthias Kragh
发明人： Patrick Maria Franciscus Derkx , Anja Kellet-Smith Hemmingen , Rie Mejldal , Bo Spange Sørensen , Karsten Matthias Kragh
CPC分类号： C12N9/2417 , C12N9/2411 , C12N9/2414
摘要： We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 307 to lysine (K) or arginine (R), with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide further comprises an amino acid substitution at position 70, preferably G70D. The amino acid at positions 272 and 303 of the sequence of the are preferably histidine (H) and glycine (G).
摘要翻译： 我们描述了可从具有非麦芽糖外切酶活性的亲本多肽衍生的PS4变体多肽，其中PS4变体多肽包含307位氨基酸取代赖氨酸（K）或精氨酸（R），参考位置编号 优选地，PS4变体多肽还包含70位氨基酸取代，优选G70D。 序列272和303位氨基酸优选为组氨酸（H）和甘氨酸（G）。
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公开(公告)号：US08802398B2
公开(公告)日：2014-08-12
申请号：US12915853
申请日：2010-10-29
申请人： Jorn Dalgaard Mikkelsen , Karsten Matthias Kragh , Rene Mikkelsen , Patrick Maria Franciscus Derkx , Shukun Yu , Harm Mulder , Igor Nikolaev
发明人： Jorn Dalgaard Mikkelsen , Karsten Matthias Kragh , Rene Mikkelsen , Patrick Maria Franciscus Derkx , Shukun Yu , Harm Mulder , Igor Nikolaev
CPC分类号： C12N11/14 , C12N9/0006 , C12P19/00
摘要： A method of oxidizing a saccharide, by contacting the saccharide with an alcohol dehydrogenase (ADH) enzyme selected from a quinone redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide (NAD+) redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide phosphate (NADP+) redox cofactor-dependent ADH, and any combination thereof is described. An oxidized saccharide obtainable by the method and products, in particular food products and paper products, containing the oxidized saccharide, are also described.
摘要翻译： 通过使糖类与选自醌氧化还原辅因子ADH，烟酰胺腺嘌呤二核苷酸（NAD +）氧化还原辅因子ADH，烟酰胺腺嘌呤二核苷酸磷酸（NADP +））的醇脱氢酶（ADH）酶接触来进行氧化糖的方法， 描述了氧化还原辅因子依赖性ADH及其任何组合。 还描述了通过该方法获得的氧化糖，特别是含有氧化糖的食品和纸制品。
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