公开(公告)号：US20070015722A1

公开(公告)日：2007-01-18

申请号：US10561324

申请日：2004-06-03

申请人： Brian Kraynack ,  Brenda Baker ,  Balkrishen Bhat ,  Eric Swayze ,  Richard Griffey

发明人： Brian Kraynack ,  Brenda Baker ,  Balkrishen Bhat ,  Eric Swayze ,  Richard Griffey

IPC分类号： A61K48/00

CPC分类号： C12N15/111 ,  C12N15/113 ,  C12N2310/315 ,  C12N2310/317 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2320/51

摘要： The present invention provides double stranded compositions that have a region that is complementary to a target nucleic acid. One of the targeting strand or the second strand comprises linked ribofuranosyl nucleosides and the other strand comprises linked modified nucleosides that have 3′-endo conformational geometry. The strands can be linked together or separate and may contain additional groups. The present invention also provides methods of using the compositions for modulating gene expression.

摘要翻译： 本发明提供具有与靶核酸互补的区域的双链组合物。 靶向链或第二链中的一个包含连接的呋喃核糖核苷，另一条链包含具有3'端构象几何形状的连接的修饰核苷。 链可以连接在一起或分离，并且可以含有另外的基团。 本发明还提供使用该组合物调节基因表达的方法。

公开(公告)号：US20050118605A9

公开(公告)日：2005-06-02

申请号：US10700884

申请日：2003-11-04

申请人： Brenda Baker ,  Anne Eldrup ,  Muthiah Manoharan ,  Balkrishen Bhat ,  Richard Griffey ,  Eric Swayze ,  Stanley Crooke ,  Thazha Prakash ,  Kallanthottathil Rajeev

发明人： Brenda Baker ,  Anne Eldrup ,  Muthiah Manoharan ,  Balkrishen Bhat ,  Richard Griffey ,  Eric Swayze ,  Stanley Crooke ,  Thazha Prakash ,  Kallanthottathil Rajeev

IPC分类号： A61K48/00 ,  C12Q1/68

CPC分类号： C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/333 ,  C12N2310/336 ,  C12N2320/50

摘要： Oligomer compositions comprising first and second oligomers are provided wherein at least a portion of the first oligomer is capable of hybridizing with at least a portion of the second oligomer, at least a portion of the first oligomer is complementary to and capble of hybridizing to a selected target nucleic acid, and at least one of the first or second oligomers has a modified base for binding to an adenine or guanine base in the opposite strand. Oligonucleotide/protein compositions are also provided comprising an oligomer complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC), wherein the oligomer has a modified base for binding to an adenine or guanine base in the opposite strand.

公开(公告)号：US20050053976A1

公开(公告)日：2005-03-10

申请号：US10859825

申请日：2004-06-03

申请人： Brenda Baker ,  Anne Eldrup ,  Muthiah Manoharan ,  Balkrishen Bhat ,  Richard Griffey ,  Eric Swayze ,  Stanley Crooke

发明人： Brenda Baker ,  Anne Eldrup ,  Muthiah Manoharan ,  Balkrishen Bhat ,  Richard Griffey ,  Eric Swayze ,  Stanley Crooke

IPC分类号： A61K48/00 ,  C07H21/02 ,  C12Q1/68

CPC分类号： A61K31/713

摘要： Oligomer compositions comprising first and second oligomers are provided wherein at least a portion of the first oligomer is capable of hybridizing with at least a portion of the second oligomer, at least a portion of the first oligomer is complementary to and capble of hybridizing to a selected target nucleic acid, and at least one of the first or second oligomers includes at least one nucleotide comprising a chimeric organic composition. Oligomer/protein compositions are also provided comprising an oligomer complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC), wherein at least one nucleotide comprising a chimeric organic composition.

摘要翻译： 提供包含第一和第二寡聚物的低聚物组合物，其中至少一部分第一寡聚体能够与至少一部分第二寡聚物杂交，至少一部分第一寡聚体与所选择的寡核苷酸杂交互补和杂交 靶核酸，并且所述第一或第二寡聚体中的至少一个包含至少一个包含嵌合有机组合物的核苷酸。 还提供寡聚物/蛋白质组合物，其包含与选定的靶核酸互补并且能够与选定的靶核酸杂交的寡聚体和至少一种包含至少一部分RNA诱导的沉默复合物（RISC）的蛋白质，其中至少一个核苷酸包含 嵌合有机组合物。

公开(公告)号：US20050080246A1

公开(公告)日：2005-04-14

申请号：US10860265

申请日：2004-06-03

申请人： Charles Allerson ,  Balkrishen Bhat ,  Anne Eldrup ,  Muthiah Manoharan ,  Richard Griffey ,  Brenda Baker ,  Eric Swayze

发明人： Charles Allerson ,  Balkrishen Bhat ,  Anne Eldrup ,  Muthiah Manoharan ,  Richard Griffey ,  Brenda Baker ,  Eric Swayze

IPC分类号： A61K48/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/113 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/323 ,  C12N2320/30 ,  C12N2320/51

摘要： The present invention provides compositions comprising at least one oligomeric compound comprising an alternating motif and further include a region that is complementary to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In preferred embodiments the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression.

摘要翻译： 本发明提供包含至少一种包含交替基序的低聚化合物并且还包括与核酸靶互补的区域的组合物。 该组合物可用于靶向选定的核酸分子并调节一个或多个基因的表达。 在优选的实施方案中，本发明的组合物与靶RNA的一部分杂交导致靶RNA的正常功能丧失。 本发明还提供了调节基因表达的方法。

公开(公告)号：US20050026160A1

公开(公告)日：2005-02-03

申请号：US10701007

申请日：2003-11-04

申请人： Charles Allerson ,  Balkrishen Bhat ,  Anne Eldrup ,  Muthiah Manoharan ,  Richard Griffey ,  Brenda Baker ,  Eric Swayze

发明人： Charles Allerson ,  Balkrishen Bhat ,  Anne Eldrup ,  Muthiah Manoharan ,  Richard Griffey ,  Brenda Baker ,  Eric Swayze

IPC分类号： C07H21/00 ,  C07H21/04 ,  C12N15/113 ,  C12Q1/68

CPC分类号： C07H21/00 ,  C07H21/04 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/323 ,  C12N2310/343 ,  C12N2310/3521 ,  C12N2310/3533 ,  C12N2320/51

摘要： The present invention provides compositions comprising at least one oligomeric compound comprising an alternating motif and further include a region that is complementary to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In preferred embodiments the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression.

公开(公告)号：US20050261218A1

公开(公告)日：2005-11-24

申请号：US10909125

申请日：2004-07-30

申请人： Christine Esau ,  Bridget Lollo ,  C. Bennett ,  Susan Freier ,  Richard Griffey ,  Brenda Baker ,  Timothy Vickers ,  Eric Marcusson ,  Erich Koller ,  Eric Swayze ,  Ravi Jain ,  Balkrishen Bhat ,  Eigen Peralta

发明人： Christine Esau ,  Bridget Lollo ,  C. Bennett ,  Susan Freier ,  Richard Griffey ,  Brenda Baker ,  Timothy Vickers ,  Eric Marcusson ,  Erich Koller ,  Eric Swayze ,  Ravi Jain ,  Balkrishen Bhat ,  Eigen Peralta

IPC分类号： A61K20060101 ,  A61K48/00 ,  C07H21/02 ,  C12N15/11 ,  C12N15/113

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/113 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/31 ,  C12N2310/315 ,  C12N2310/317 ,  C12N2310/3181 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2310/351 ,  C12N2310/3515 ,  C12N2320/11 ,  C12N2320/30 ,  C12N2320/50 ,  C12N2310/3525

摘要： Compounds, compositions and methods are provided for modulating the expression and function of small non-coding RNAs. The compositions comprise oligomeric compounds, targeted to small non-coding RNAs. Methods of using these compounds for modulation of small non-coding RNAs as well as downstream targets of these RNAs and for diagnosis and treatment of disease associated with small non-coding RNAs are also provided.

摘要翻译： 提供化合物，组合物和方法用于调节小非编码RNA的表达和功能。 组合物包含靶向小的非编码RNA的寡聚化合物。 还提供了使用这些化合物调节小非编码RNA以及这些RNA的下游靶标以及用于诊断和治疗与小非编码RNA相关的疾病的方法。

公开(公告)号：US20050042647A1

公开(公告)日：2005-02-24

申请号：US10860455

申请日：2004-06-03

申请人： Brenda Baker ,  Anne Eldrup ,  Muthiah Manoharan ,  Balkrishen Bhat ,  Richard Griffey ,  Eric Swayze ,  Stanley Crooke ,  Thazha Prakash

发明人： Brenda Baker ,  Anne Eldrup ,  Muthiah Manoharan ,  Balkrishen Bhat ,  Richard Griffey ,  Eric Swayze ,  Stanley Crooke ,  Thazha Prakash

IPC分类号： A61K48/00 ,  C07H21/02 ,  C12Q1/68

CPC分类号： C07H21/02 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/319 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/342 ,  C12N2310/343 ,  C12N2310/335 ,  C12N2310/3521 ,  C12N2310/321 ,  C12N2310/3525

摘要： Oligonucleotide compositions comprising first and second oligonucleotides are provided wherein at least a portion of the first oligonucleotide is capable of hybridizing with at least a portion of the second oligonucleotide, at least a portion of the first oligonucleotide is complementary to and capble of hybridizing to a selected target nucleic acid, and at least one of the first or second oligonucleotides includes at least one nucleotide having a modified phosphorous-containing internucleoside linkage. Oligonucleotide/protein compositions are also provided comprising an oligonucleotide complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC), wherein at least one nucleotide of the oligonucleotide has a modified phosphorous-containing internucleoside linkage.

摘要翻译： 提供了包含第一和第二寡核苷酸的寡核苷酸组合物，其中至少一部分第一寡核苷酸能够与至少一部分第二寡核苷酸杂交，第一寡核苷酸的至少一部分与选定的 靶核酸，并且第一或第二寡核苷酸中的至少一个包含至少一个具有修饰的含磷核苷间键的核苷酸。 还提供了寡核苷酸/蛋白质组合物，其包含与选定的靶核酸互补并且能够与选定的靶核酸杂交的寡核苷酸和至少一种包含至少一部分RNA诱导的沉默复合物（RISC）的蛋白质，其中至少一个核苷酸 寡核苷酸具有修饰的含磷核苷键。

公开(公告)号：US20050037370A1

公开(公告)日：2005-02-17

申请号：US10700884

申请日：2003-11-04

申请人： Brenda Baker ,  Anne Eldrup ,  Muthiah Manoharan ,  Balkrishen Bhat ,  Richard Griffey ,  Eric Swayze ,  Stanley Crooke ,  Thazha Prakash ,  Kallanthottathil Rajeev

发明人： Brenda Baker ,  Anne Eldrup ,  Muthiah Manoharan ,  Balkrishen Bhat ,  Richard Griffey ,  Eric Swayze ,  Stanley Crooke ,  Thazha Prakash ,  Kallanthottathil Rajeev

IPC分类号： A61K48/00 ,  C12Q1/68

CPC分类号： C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/333 ,  C12N2310/336 ,  C12N2320/50

摘要： Oligomer compositions comprising first and second oligomers are provided wherein at least a portion of the first oligomer is capable of hybridizing with at least a portion of the second oligomer, at least a portion of the first oligomer is complementary to and capble of hybridizing to a selected target nucleic acid, and at least one of the first or second oligomers has a modified base for binding to an adenine or guanine base in the opposite strand. Oligonucleotide/protein compositions are also provided comprising an oligomer complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC), wherein the oligomer has a modified base for binding to an adenine or guanine base in the opposite strand.

摘要翻译： 提供包含第一和第二寡聚物的低聚物组合物，其中至少一部分第一寡聚体能够与至少一部分第二寡聚物杂交，至少一部分第一寡聚体与所选择的寡核苷酸杂交互补和杂交 靶核酸，并且第一或第二寡聚体中的至少一个具有与相对链中的腺嘌呤或鸟嘌呤碱基结合的修饰碱基。 还提供了寡核苷酸/蛋白质组合物，其包含与选定的靶核酸互补并且能够与选定的靶核酸杂交的寡聚体和至少一种包含RNA诱导的沉默复合物（RISC）的至少一部分的蛋白质，其中所述寡聚物具有修饰的碱基 用于与相对链中的腺嘌呤或鸟嘌呤碱基结合。

公开(公告)号：US20080039618A1

公开(公告)日：2008-02-14

申请号：US11871436

申请日：2007-10-12

申请人： Charles Allerson ,  Balkrishen Bhat ,  Anne Eldrup ,  Muthiah Manoharan ,  Richard Griffey ,  Brenda Baker ,  Eric Swayze

发明人： Charles Allerson ,  Balkrishen Bhat ,  Anne Eldrup ,  Muthiah Manoharan ,  Richard Griffey ,  Brenda Baker ,  Eric Swayze

IPC分类号： C07H21/04

CPC分类号： C07H21/00 ,  C07H21/04 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/323 ,  C12N2310/343 ,  C12N2310/3521 ,  C12N2310/3533 ,  C12N2320/51

摘要： Compositions comprising first and second oligomers are provided wherein at least a portion of the first oligomer is capable of hybridizing with at least a portion of the second oligomer, at least a portion of the first oligomer is complementary to and capable of hybridizing to a selected target nucleic acid, and at least one of the first or second oligomers includes a modification comprising a polycyclic sugar surrogate. Oligomer/protein compositions are also provided comprising an oligomer complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC), wherein at least one nucleoside of the oligomer has a polycyclic sugar surrogate modification.

摘要翻译： 提供包含第一和第二寡聚体的组合物，其中至少一部分第一寡聚体能够与至少一部分第二寡聚物杂交，至少一部分第一寡聚体与选定的靶互补并能够与选定的靶 核酸，并且第一或第二寡聚体中的至少一种包括包含多环糖替代物的修饰物。 还提供寡聚物/蛋白质组合物，其包含与选定的靶核酸互补并且能够与选定的靶核酸杂交的寡聚体和至少一种包含至少一部分RNA诱导的沉默复合物（RISC）的蛋白质，其中至少一种核苷 低聚物具有多环糖替代物修饰。

公开(公告)号：US20060084094A1

公开(公告)日：2006-04-20

申请号：US11221001

申请日：2005-09-07

申请人： C. Bennett ,  Robert McKay ,  Brett Monia ,  Brenda Baker ,  Namir Sioufi ,  Richard Griffey ,  Balkrishen Bhat ,  Eric Swayze

发明人： C. Bennett ,  Robert McKay ,  Brett Monia ,  Brenda Baker ,  Namir Sioufi ,  Richard Griffey ,  Balkrishen Bhat ,  Eric Swayze

IPC分类号： C12Q1/68 ,  C07H21/02

CPC分类号： C12N15/111 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/323 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2310/3515 ,  C12N2320/11 ,  C12N2320/32 ,  C12Y301/03048 ,  C12N2310/3521 ,  C12N2310/3525

摘要： The present invention provides methods of identifying oligomeric compounds, such as siRNA and double-stranded RNA compounds, having bioactivity in vivo, and kits.

摘要翻译： 本发明提供了鉴定体内具有生物活性的寡聚化合物如siRNA和双链RNA化合物的方法以及试剂盒。