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公开(公告)号：US07250274B2
公开(公告)日：2007-07-31
申请号：US10735149
申请日：2003-12-12
申请人： Chung Chan , Bruce L. Zamost , Douglas C. Covert , Hong Y. Liu , Karen S. De Jongh , Jeffrey D. Meyer , Susan D. Holderman
发明人： Chung Chan , Bruce L. Zamost , Douglas C. Covert , Hong Y. Liu , Karen S. De Jongh , Jeffrey D. Meyer , Susan D. Holderman
IPC分类号： C12P21/02
CPC分类号： C07K14/54
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the IL-21 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains transformed with an IL-21 expression vector.
摘要翻译： 描述了用于大规模生产IL-21的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-21编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 使用表达载体，在补料分批发酵中，在大肠杆菌中产生IL-21基因至大于1g / L的水平。 还包括用IL-21表达载体转化的OmpT缺陷型大肠杆菌菌株。
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公开(公告)号：US07622561B2
公开(公告)日：2009-11-24
申请号：US11539045
申请日：2006-10-05
申请人： Bruce L. Zamost , Hong Y. Liu , Karen S. De Jongh
发明人： Bruce L. Zamost , Hong Y. Liu , Karen S. De Jongh
CPC分类号： C07K14/54
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Using the expression vectors, the IL-21 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains transformed with an IL-21 expression vector.
摘要翻译： 描述了用于大规模生产IL-21的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-21编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 使用表达载体，在补料分批发酵中，在大肠杆菌中产生IL-21基因至大于1g / L的水平。 还包括用IL-21表达载体转化的OmpT缺陷型大肠杆菌菌株。
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公开(公告)号：US20080096252A1
公开(公告)日：2008-04-24
申请号：US11538688
申请日：2006-10-04
摘要： The expression vectors and methods using an E. Coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US06258559B1
公开(公告)日：2001-07-10
申请号：US09532803
申请日：2000-03-21
申请人： Bruce L. Zamost
发明人： Bruce L. Zamost
IPC分类号： C12P2106
摘要： Methylotrophic yeasts are useful hosts for the production of commercially valuable recombinant proteins. However, the development of large-scale cultures of recombinant methylotrophic yeasts has been hindered by the formation of precipitation in culture media. A new soluble minimal medium overcomes this problem. Moreover, new feeding schemes provide cultures of high biomass, which produce biologically active recombinant protein.
摘要翻译： 甲基营养酵母是生产有价值的重组蛋白质的有用宿主。 然而，重组甲基营养酵母的大规模培养物的发育受到培养基中沉淀的形成的阻碍。 一种新的可溶性基本培养基克服了这个问题。 此外，新的饲养方案提供高生物量的培养物，其产生生物活性重组蛋白。
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公开(公告)号：US07759092B2
公开(公告)日：2010-07-20
申请号：US12537935
申请日：2009-08-07
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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6.发明授权Thermostable arabino furanoside produced by Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661 失效由嗜热脂肪芽孢杆菌NRRL B-18659，嗜热脂肪芽孢杆菌NRRL B-18660和嗜热脂肪芽孢杆菌NRRL B-18661产生的热稳定的阿拉伯呋喃糖苷
公开(公告)号：US5491087A
公开(公告)日：1996-02-13
申请号：US418044
申请日：1995-04-06
申请人： Bruce L. Zamost , Dana D. Elm
发明人： Bruce L. Zamost , Dana D. Elm
CPC分类号： C12Y302/01032 , C12N9/2482 , C12Y302/01008 , C12Y302/01037 , C12Y302/01055 , Y10S435/832
摘要： An isolated arabinofuranosidase from Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661 is disclosed. The arabinofuranosidase has a maximum activity at about pH 6.0 and at about 65.degree. C., maintains at least about 50% of its maximum activity at 70.degree. C. and pH 7.0 after 80 minutes, and has an isoelectric point of about 4.4. The arabinfuranosidase can be used in a method of hydrolyzing xylan present in wood pulp at temperatures of at least about 60.degree. C. and a pH of at least about 7.0. The arabinofuranosidase is used along with at least two xylanases and a xylosidase isolated from the above Bacillus stearothermophilus strains.
摘要翻译： 公开了来自嗜热脂肪芽孢杆菌NRRL B-18659，嗜热脂肪芽孢杆菌NRRL B-18660和嗜热脂肪芽孢杆菌NRRL B-18661的分离的阿拉伯呋喃糖苷酶。 阿拉伯呋喃糖苷酶在约pH 6.0和约65℃下具有最大活性，80分钟后在70℃和pH 7.0保持其最大活性的至少约50％，等电点约4.4。 阿拉伯呋喃糖苷酶可用于在至少约60℃的温度和至少约7.0的pH下水解木浆中存在的木聚糖的方法。 阿拉伯呋喃糖苷酶与至少两种木聚糖酶和从上述嗜热脂肪芽孢杆菌菌株分离的木糖苷酶一起使用。
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公开(公告)号：US20120015403A1
公开(公告)日：2012-01-19
申请号：US13114566
申请日：2011-05-24
IPC分类号： C12P21/02
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US07968315B2
公开(公告)日：2011-06-28
申请号：US12701358
申请日：2010-02-05
摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.
摘要翻译： 描述了用于大规模生产IL-29的大肠杆菌表达系统的表达载体和方法。 载体利用具有核苷酸特异性变化的IL-29编码序列，以优化用于大肠杆菌翻译的密码子和mRNA二级结构。 还包括产生，纯化和聚乙二醇化IL-29多肽的方法。
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公开(公告)号：US06750045B2
公开(公告)日：2004-06-15
申请号：US10201168
申请日：2002-07-23
申请人： Bruce L. Zamost
发明人： Bruce L. Zamost
IPC分类号： C12P2102
CPC分类号： C12N1/18 , C07K14/755
摘要： A yeast fermentation medium that does not contain yeast cell extract but is comprised of a mixture of oleic acid, lactic acid and palmitic acid. Using this fermentation medium yields of factor XIII were increased 250%.
摘要翻译： 不含酵母细胞提取物但由油酸，乳酸和棕榈酸的混合物组成的酵母发酵培养基。 使用该发酵培养基，因子XIII的产量增加了250％。
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10.发明授权Thermostable xylosidase produced by Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661 失效由嗜热脂肪芽孢杆菌NRRL B-18659，嗜热脂肪芽孢杆菌NRRL B-18660和嗜热脂肪芽孢杆菌NRRL B-18661产生的热稳定性木糖苷酶
公开(公告)号：US5489526A
公开(公告)日：1996-02-06
申请号：US418331
申请日：1995-04-06
申请人： Bruce L. Zamost , Dana D. Elm
发明人： Bruce L. Zamost , Dana D. Elm
CPC分类号： C12Y302/01032 , C12N9/2482 , C12Y302/01008 , C12Y302/01037 , C12Y302/01055 , Y10S435/832
摘要： An isolated xylosidase from Bacillus stearothermophilus NRRL B-18659, Bacillus stearothermophilus NRRL B-18660 and Bacillus stearothermophilus NRRL B-18661 is disclosed. The xylosidase has a maximum activity at about pH 6.0 and at about 75.degree. C., maintains at least about 60% of its maximum activity at about 65.degree. C. and pH 7 after 4 hours, is resistant to end-product inhibition maintaining over 75% of maximum activity in the presence of 1 molar xylose and has an isoelectric point of about 5.0. The xylosidase can be used in a method of hydrolyzing xylan present in wood pulp at temperatures of at least about 60.degree. C. and a pH of at least about 7.0. The xylosidase is used along with at least two xylanases and an arabinofuranosidase isolated from the above Bacillus stearothermophilus strains.
摘要翻译： 公开了来自嗜热脂肪芽孢杆菌NRRL B-18659，嗜热脂肪芽孢杆菌NRRL B-18660和嗜热脂肪芽孢杆菌NRRL B-18661的分离的木糖苷酶。 木糖苷酶在约pH6.0和约75℃下具有最大活性，在约65℃保持其最大活性的至少约60％，4小时后保持pH7，耐受最终产物抑制 在1摩尔木糖存在下具有75％的最大活性，并具有约5.0的等电点。 木糖苷酶可用于在至少约60℃的温度和至少约7.0的pH下水解木浆中存在的木聚糖的方法。 木糖苷酶与至少两种木聚糖酶和从上述嗜热脂肪芽孢杆菌菌株分离的阿拉伯呋喃糖苷酶一起使用。
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