公开(公告)号：US20200078460A1

公开(公告)日：2020-03-12

申请号：US16568886

申请日：2019-09-12

Applicant: Carnegie Mellon University

Inventor： Marcel P. Bruchez ,  Jianjun He ,  Yi Wang

IPC: A61K41/00 ,  C09B11/22 ,  C07K14/71 ,  C07K14/00 ,  A61N5/06 ,  C09B11/10 ,  C07C233/40 ,  C07C233/36 ,  C07C211/43 ,  A61K47/64

Abstract: Provided herein is a two-component photosensitizer, which demonstrated robust and selective killing effects for transfected HEK cells and affibody targeted A431 cancer cells when exposed to near infrared light excitation. Free MG2I is a pure and stable fluorogen; it is easy to synthesize and modify, and has no toxicity to cells. Unlike conventional photosensitizers, the dye and FAP itself has no photosensitizing effect until they are bound. Also unlike other activation methods, the activation step is achieved by adding the fluorogen, not the presence of the targeted molecule, requiring an ‘active’ activation instead of a ‘passive’ activation. This method offers the ability to locally switch-on and selective generation of singlet oxygen at the target site and can be used for a wide variety of molecular targets.

公开(公告)号：US10434177B2

公开(公告)日：2019-10-08

申请号：US15527061

申请日：2015-11-17

Applicant: Carnegie Mellon University

Inventor： Marcel P. Bruchez ,  Jianjun He ,  Yi Wang

IPC: A61K41/00 ,  C07C211/43 ,  C07C233/36 ,  C07C233/40 ,  C09B11/10 ,  A61K47/64 ,  A61N5/06 ,  C07K14/00 ,  C07K14/71 ,  C09B11/22

Abstract: Provided herein is a two-component photosensitizer, which demonstrated robust and selective killing effects for transfected HEK cells and affibody targeted A431 cancer cells when exposed to near infrared light excitation. Free MG2I is a pure and stable fluorogen; it is easy to synthesize and modify, and has no toxicity to cells. Unlike conventional photosensitizers, the dye and FAP itself has no photosensitizing effect until they are bound. Also unlike other activation methods, the activation step is achieved by adding the fluorogen, not the presence of the targeted molecule, requiring an ‘active’ activation instead of a ‘passive’ activation. This method offers the ability to locally switch-on and selective generation of singlet oxygen at the target site and can be used for a wide variety of molecular targets.

公开(公告)号：US10946098B2

公开(公告)日：2021-03-16

申请号：US16568886

申请日：2019-09-12

Applicant: Carnegie Mellon University

Inventor： Marcel P. Bruchez ,  Jianjun He ,  Yi Wang

IPC: A61K41/00 ,  A61K47/64 ,  C07C211/43 ,  C07C233/36 ,  C07C233/40 ,  C09B11/10 ,  A61N5/06 ,  C07K14/00 ,  C07K14/71 ,  C09B11/22

Abstract: Provided herein is a two-component photosensitizer, which demonstrated robust and selective killing effects for transfected HEK cells and affibody targeted A431 cancer cells when exposed to near infrared light excitation. Free MG2I is a pure and stable fluorogen; it is easy to synthesize and modify, and has no toxicity to cells. Unlike conventional photosensitizers, the dye and FAP itself has no photosensitizing effect until they are bound. Also unlike other activation methods, the activation step is achieved by adding the fluorogen, not the presence of the targeted molecule, requiring an ‘active’ activation instead of a ‘passive’ activation. This method offers the ability to locally switch-on and selective generation of singlet oxygen at the target site and can be used for a wide variety of molecular targets.

公开(公告)号：US20180280510A1

公开(公告)日：2018-10-04

申请号：US15527061

申请日：2015-11-17

Applicant: Carnegie Mellon University

Inventor： Marcel P. Bruchez ,  Jianjun He ,  Yi Wang

IPC: A61K41/00 ,  C07K14/71 ,  C07K14/00 ,  C09B11/22 ,  A61N5/06

CPC classification number: A61K41/0057 ,  A61K47/64 ,  A61N5/062 ,  A61N2005/0659 ,  C07C211/43 ,  C07C233/36 ,  C07C233/40 ,  C07K14/001 ,  C07K14/71 ,  C07K2317/622 ,  C07K2319/035 ,  C07K2319/30 ,  C09B11/10 ,  C09B11/22

Abstract: Provided herein is a two-component photosensitizer, which demonstrated robust and selective killing effects for transfected HEK cells and affibody targeted A431 cancer cells when exposed to near infrared light excitation. Free MG2I is a pure and stable fluorogen; it is easy to synthesize and modify, and has no toxicity to cells. Unlike conventional photosensitizers, the dye and FAP itself has no photosensitizing effect until they are bound. Also unlike other activation methods, the activation step is achieved by adding the fluorogen, not the presence of the targeted molecule, requiring an ‘active’ activation instead of a ‘passive’ activation. This method offers the ability to locally switch-on and selective generation of singlet oxygen at the target site and can be used for a wide variety of molecular targets.

公开(公告)号：US11174325B2

公开(公告)日：2021-11-16

申请号：US16475886

申请日：2018-01-11

Applicant: CARNEGIE MELLON UNIVERSITY

Inventor： Marco Fantin ,  Pawel Chmielarz ,  Yi Wang ,  Francesca Lorandi ,  Krzysztof Matyjaszewski

IPC: C08F2/38 ,  C08F2/26 ,  C08F4/10 ,  C08F4/40 ,  C08F220/18

Abstract: Systems and methods for Atom Transfer Radical Polymerization (ATRP) emulsion polymerization are provided. The ATRP emulsion polymerization comprises a suspending medium, a dispersed medium, a surfactant, a transition metal compound in a higher oxidation state, a ligand, and an ATRP initiator. The transition metal compound is capable of forming a catalyst complex in a presence of the ligand. The catalyst complex is soluble in the suspending medium and is capable of forming an ionic complex with the surfactant. The ionic complex is capable of moving between the suspending medium and the dispersed medium. A portion of the transition metal compound in the higher oxidation state within a portion of the catalyst complex is reduced by a physical and/or a chemical procedure thereby initiating a polymerization of one or more radically (co)polymerizable monomers by reaction with the initiator.