公开(公告)号：US20160319344A1

公开(公告)日：2016-11-03

申请号：US15106067

申请日：2014-12-19

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  ECOLE NORMALE SUPERIEURE ,  UNIVERSITE D'EVRY VAL D'ESSONNE

发明人： Vincent CROQUETTE ,  Maria MANOSAS ,  Jerome MATHE ,  David BENSIMON ,  Jean-Francois ALLEMAND

IPC分类号： C12Q1/68 ,  G01N27/447

CPC分类号： C12Q1/6869 ,  G01N27/4473 ,  G01N27/44791 ,  C12Q2521/513 ,  C12Q2565/631

摘要： The present invention relates to a system of nanopore sequencing using nucleic-acid binding proteins wherein the activity of the said protein and the passage of the sequenced nucleic acid molecule through the nanopore are coordinated. In particular, the present invention enables an easy synchronization of nanopore capture with polynucleotide unwinding, which in turn affords an easy way to repeat the whole process of capture and nucleotide characterization.

摘要翻译： 本发明涉及使用核酸结合蛋白的纳米孔测序系统，其中所述蛋白质的活性和测序的核酸分子通过纳米孔的通过被协调。 特别地，本发明能够使纳米孔捕获与多核苷酸展开方便地同步，这又提供了重复捕获和核苷酸表征的整个过程的简单方法。

公开(公告)号：US20150362488A1

公开(公告)日：2015-12-17

申请号：US14761944

申请日：2014-01-22

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  ECOLE NORMALE SUPERIEURE ,  UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6)

发明人： David BENSIMON ,  Vincent CROQUETTE ,  Harold GOUET ,  Jean-Francois ALLEMAND ,  Fang-Yuan DING

IPC分类号： G01N33/557 ,  C12Q1/68

CPC分类号： G01N33/557 ,  C12Q1/6883 ,  C12Q2600/154 ,  C12Q2600/156

摘要： The present invention relates to a method for determining whether a protein binds to a specific DNA sequence. This method is useful in particular for identifying modifications to the DNA sequence (e.g. methylations) via the binding of proteins that specifically recognize those modifications (e.g. antibodies), but also to identify the binding sequence on DNA of a variety of proteins.

摘要翻译： 本发明涉及一种用于确定蛋白质是否结合特定DNA序列的方法。 该方法特别用于通过特异性识别那些修饰（例如抗体）的蛋白质的结合识别对DNA序列的修饰（例如甲基化），而且可以鉴定多种蛋白质的DNA上的结合序列。

公开(公告)号：US20220266249A1

公开(公告)日：2022-08-25

申请号：US17596986

申请日：2020-06-26

申请人： PARIS SCIENCES ET LETTRES ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  SORBONNE UNIVERSITE ,  UNIVERSITE DE PARIS

发明人： Gaël RADOU ,  Thibault VIEILLE ,  Vincent CROQUETTE

IPC分类号： B01L3/00 ,  B01L9/00 ,  G01B7/30 ,  G01D5/14 ,  G02B21/02 ,  G02B21/26

摘要： A device positioning an object according to a given direction, including a frame, a holderfor holding the object, the holder being movable relative to the frame according to the given directio, a main lever extending between a first point and a second point, said main lever being rotatably mounted to the frame via a first pivot link connecting the main lever and the frame at the first point of the main lever, the main lever being connected to the holder at a third point which is arranged between the first point and the second point, so that a displacement of the second point of the main lever relative to the frame according to the given direction causes displacement of the holder in said given direction.

公开(公告)号：US20180105872A1

公开(公告)日：2018-04-19

申请号：US15570070

申请日：2016-05-04

申请人： PARIS SCIENCES ET LETTRES - QUARTIER LATIN ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6) ,  UNIVERSITE PARIS DIDEROT PARIS 7

发明人： Vincent CROQUETTE ,  Jimmy OUELLET

IPC分类号： C12Q1/6834 ,  C12Q1/6832 ,  C12Q1/6816

CPC分类号： C12Q1/6834 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6874 ,  C12Q2523/305 ,  C12Q2525/301 ,  C12Q2563/143

摘要： The present invention relates to a method of preparation of substrates for nucleic acid sequencing reactions. More specifically, the present invention provides a new method of preparing hairpins using force-induced strand invasion. Hairpins prepared by this method and methods of nucleic acid analysis using these hairpins are also part of the present invention.

公开(公告)号：US20230028605A1

公开(公告)日：2023-01-26

申请号：US17784639

申请日：2020-11-27

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  ECOLE NORMALE SUPERIEURE DE PARIS ,  SORBONNE UNIVERSITE

发明人： Raja CHOUKET ,  Vincent CROQUETTE ,  Agathe ESPAGNE ,  Ludovic JULLIEN ,  Annie LEMARCHAND ,  Thomas LE SAUX ,  Agnès PELISSIER-TANON ,  Ruikang ZHANG

IPC分类号： G01N21/64

摘要： A method for detecting a reversibly photoswitchable chemical species in a sample, includes the steps of: a) illuminating the sample with light suitable to be absorbed by the chemical species triggering a reaction affecting an optical property of the chemical species, the first light being periodically-modulated at a fundamental modulation frequency; b) measuring the evolution of the optical property; c) extracting at least one of an in-phase component at a frequency which is an even multiple of the fundamental modulation frequency; and a quadrature component at a frequency which is an odd multiple of the fundamental modulation frequency of a signal representing the evolution; and d) using the extracted component or components for detecting the chemical species. An apparatus for carrying out the method is also provided.

公开(公告)号：US20160356716A1

公开(公告)日：2016-12-08

申请号：US15034402

申请日：2014-11-21

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  INSTITUT CURIE ,  ECOLE NORMALE SUPERIEURE DE PARIS ,  UNIVERSITE PIERRE ET MARIE CURIE

发明人： Ludovic JULLIEN ,  Thomas LE SAUX ,  Arnaud GAUTIER ,  Vincent CROQUETTE ,  Nath SARANG ,  Pencheng WANG ,  Jérôme QUERARD ,  Samantha BRIGHT

IPC分类号： G01N21/64 ,  A61K49/00 ,  G01N33/53

CPC分类号： G01N21/6408 ,  A61K49/0017 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/5306 ,  G01N2201/12

摘要： Method for detection of at least one reversibly photo-convertible fluorescent species (P), comprising the following steps: a) illumination of a sample comprising said or at least one of said reversibly photo-convertible fluorescent species by a periodically modulated illuminating light (FEX); and b) detection of fluorescent emission (FLU) emitted by the sample thus illuminated; characterized in that the method further comprises the following step: c) extraction of the amplitude (IFOUt) of the intensity component of said fluorescent emission exhibiting the same periodicity as said periodically modulated illuminating light and a phase quadrature with respect to the same; and in that the mean intensity of said illuminating light and the modulation frequency of the same are chosen in such a way as to maximise said amplitude of the intensity component of said fluorescent emission.

摘要翻译： 用于检测至少一个可逆光可变换荧光物质（P）的方法，包括以下步骤：a）通过周期调制的照明光（FEX）照射包含所述可逆光可变换荧光物质中的所述或至少一种的样品 ）; 和b）由如此照射的样品发射的荧光发射（FLU）的检测; 其特征在于，所述方法还包括以下步骤：c）提取与所述周期性调制的照明光具有相同周期的所述荧光发射的强度分量的幅度（IFOUt）和相对于其的相位正交; 并且以使得所述荧光发射的强度分量的所述振幅最大化的方式来选择所述照明光的平均强度和其调制频率。