公开(公告)号：US20240377386A1

公开(公告)日：2024-11-14

申请号：US18659540

申请日：2024-05-09

Applicant: CFD Research Corporation

Inventor： David Edwin Gaddes, III ,  Trevor Jaxon Suddarth ,  Ketan Harendrakumar Bhatt

IPC: G01N33/52 ,  B01L3/00

Abstract: A diagnostic system is provided, which can include a sample tube having an input end and an output end, a microfluidic device, a heating member, and a reagent. The microfluidic device may include a port member configured to couple with the sample tube, a sample distribution member coupled with the port member and having a microfluidic network with microfluidic channels, reaction chambers fluidly coupled with the microfluidic channels, a top cover configured to receive the sample tube therein and having openings corresponding to the reaction chambers, and a bottom member configured to mate with the top cover to enclose the port member and the sample distribution member. The bottom member may include a bottom chamber for retaining a composition. The heating member may be placed in the bottom chamber, and the reagent in the reaction chambers may provide a colorimetric reading upon interaction with a target analyte in the sample.

公开(公告)号：US20210269868A1

公开(公告)日：2021-09-02

申请号：US17187267

申请日：2021-02-26

Applicant: CFD Research Corporation

Inventor： David Edwin Gaddes, III ,  Ketan Harendrakumar Bhatt ,  Balabhaskar Prabhakarpandian

IPC: C12Q1/6853 ,  C12Q1/6832 ,  C12Q1/6816

Abstract: A primer composition for amplifying a target nucleic acid of a plurality of target organisms can include: a first primer having in order: 3′-a first target specific sequence; a melt key region; and a first super primer region, wherein the first target specific sequence includes a nucleic acid sequence that specifically hybridizes with a first unique nucleic acid sequence of a first target organism over nucleic acid sequences of other organisms, the melt key region includes a nucleic acid sequence that has less than full identity with a melt key probe and a complement of the melt key region hybridizes with the melt key probe with at least one nucleotide mismatch with the melt key probe, and the first super primer region includes a nucleic acid sequence that is common to the plurality of target organisms.

公开(公告)号：US20210116338A1

公开(公告)日：2021-04-22

申请号：US17074007

申请日：2020-10-19

Applicant: CFD RESEARCH CORPORATION

Inventor： David Edwin Gaddes, III ,  Balabhaskar Prabhakarpandian ,  Ketan Harendrakumar Bhatt

IPC: G01N1/40 ,  B01L3/00

Abstract: A fluidic device can include: a plurality of fluid conduits, each fluid conduit including a first conduit portion separated from a second conduit portion; and at least one transport body that is movably positioned between the first conduit portion and the second conduit portion of each fluid conduit. The at least one transport body can include: at least one port adapted to be aligned with a first conduit portion and a second conduit portion of at least one first conduit so as to fluidly couple the first conduit portion with the second conduit portion; and at least one blocking body portion adapted to be aligned with a first conduit portion and a second conduit portion of at least one second conduit so as to fluidly isolate the first conduit portion from the second conduit portion of the at least one second conduit.

公开(公告)号：US20250161939A1

公开(公告)日：2025-05-22

申请号：US18952740

申请日：2024-11-19

Applicant: CFD Research Corporation

Inventor： David Edwin Gaddes, III ,  Trevor Jaxon Sudarth ,  Ketan Harendrakumar Bhatt

IPC: B01L3/00

Abstract: A system for analyzing a sample can include a sample tube with an input end having a removable lid and an output end, and a microfluidic device. The microfluidic device may include a port member configured to couple with the output end; a sample distribution member coupled with the port member and having a microfluidic network with microfluidic channels; reaction chambers fluidly coupled with the microfluidic channels; and a reactor member configured to generate and display light signals on a display, the light signals generated based on reactions in the reaction chambers and indicating results of the reactions.

公开(公告)号：US20240344127A1

公开(公告)日：2024-10-17

申请号：US18632071

申请日：2024-04-10

Applicant: CFD Research Corporation

Inventor： David Edwin Gaddes, III ,  Kyleigh Raye Jones

IPC: C12Q1/6876 ,  C12Q1/6853

CPC classification number: C12Q1/6876 ,  C12Q1/6853 ,  C12Q2600/16

Abstract: A method of performing an asymmetric loop-mediated isothermal amplification (LAMP) assay can include: providing the asymmetrical primer set of one of the embodiments; providing a reagent composition having a polymerase; combining the asymmetrical primer set and reagent composition with a sample having the target nucleic acid to form an amplification mixture; heating the amplification mixture to an amplification temperature within an amplification temperature range; maintaining the amplification temperature to amplify the target nucleic acid having the target sequence; and obtaining an amplified amount of the target nucleic acid having the target sequence. The asymmetrical primer set can include one primer of a primer pair to be at a lower (e.g., 10%) amount of the other primer.