公开(公告)号：US20150291932A1

公开(公告)日：2015-10-15

申请号：US14647304

申请日：2013-11-25

Applicant: Ronald Allen FARIS ,  Yulong HONG ,  Joydeep LAHIRI ,  Jin LIU ,  Olivia Grace WEEKS ,  CORNING INCORPORATED

Inventor： Ronald Allen Faris ,  Yulong Hong ,  Joydeep Lahiri ,  Jin Liu ,  Olivia Grace Weeks

IPC: C12N5/071 ,  G01N33/50 ,  C12Q1/68

CPC classification number: C12N5/067 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2533/54 ,  C12Q1/6876 ,  C12Q2600/158 ,  G01N33/5067 ,  G01N2333/90245

Abstract: Cell culture compositions containing LFM-A13 or a structurally related compound can enhance global hepatic function. For example, LFM-A13 is shown to enhance levels of a broad variety of drug metabolism enzymes, including CYP enzymes, and other hepatic enzymes. LFM-A13 is also shown to promote differentiation of stem cells into hepatocytes. LFM-A13 and structurally related compounds can be used in cell culture to enhance global drug metabolism of liver cells for enhanced in vitro study the effects of drug metabolism on other candidate drug compounds.

Abstract translation: 含有LFM-A13或结构相关化合物的细胞培养组合物可以增强全身肝功能。 例如，LFM-A13被证明可以增强多种药物代谢酶的水平，包括CYP酶和其他肝酶。 还显示LFM-A13促进干细胞分化成肝细胞。 LFM-A13和结构相关的化合物可用于细胞培养，以增强肝细胞的全球药物代谢，增强体外研究药物代谢对其他候选药物化合物的作用。

公开(公告)号：US20220062186A1

公开(公告)日：2022-03-03

申请号：US17501325

申请日：2021-10-14

Applicant: CORNING INCORPORATED

Inventor： Ronald Allen Faris ,  Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  Jin Liu ,  Gregory Roger Martin

IPC: A61K9/28

Abstract: An ex vivo cell culture sustained release composition, including: a mixture comprising: a sustenant; and a non-biodegradable binder; and a non-biodegradable and water insoluble encapsulant coat that encapsulates the mixture, as defined herein. Also disclosed is a method for sustainably providing a sustenant to a cell culture in aqueous media, including contacting a cell culture with the sustained release composition.

公开(公告)号：US20150275167A1

公开(公告)日：2015-10-01

申请号：US14663533

申请日：2015-03-20

Applicant: Corning Incorporated

Inventor： Ronald Allen Faris ,  Ann MeeJin Ferrie ,  Vasiliy Nikolaevich Goral ,  Gregory Roger Martin ,  Jin Liu

IPC: C12N5/00 ,  C12N5/071

CPC classification number: A61K9/2866

Abstract: An ex vivo cell culture sustained release composition, including: a mixture comprising: a sustenant; and a non-biodegradable binder; and a non-biodegradable and water insoluble encapsulant coat that encapsulates the mixture, as defined herein. Also disclosed is a method for sustainably providing a sustenant to a cell culture in aqueous media, including contacting a cell culture with the sustained release composition.

Abstract translation: 一种离体细胞培养持续释放组合物，包括：包含：维生素的混合物; 和不可生物降解的粘合剂; 以及包封如本文所定义的混合物的不可生物降解的水不溶性包封剂涂层。 还公开了一种用于将水分介质中的细胞培养物可持续地提供给细胞培养物的方法，包括使细胞培养物与持续释放组合物接触。

公开(公告)号：US08679843B2

公开(公告)日：2014-03-25

申请号：US13911490

申请日：2013-06-06

Applicant: Corning Incorporated

Inventor： Ronald Allen Faris ,  Vasiliy Nikolaevich Goral ,  Miya Yi-Cheng Hsieh ,  Odessa Natalie Petzold ,  Po Ki Yuen

IPC: C12N5/071

CPC classification number: G01N33/5008 ,  C12M21/08 ,  C12M23/16 ,  C12M23/24 ,  C12M23/40 ,  C12M29/10

Abstract: A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel.

Abstract translation: 微流体细胞培养装置包括细胞保留室和灌注通道。 细胞保留室具有结构化表面。 结构化表面包括主表面，多个突起从该主表面延伸到腔室中。 多个突起被布置成悬浮在主表面上方的室中培养的细胞。 第一灌注通道构造成提供流体通过通道的层流，并形成与细胞保持室连通的多个开口。 开口构造成防止来自保留腔的细胞进入灌注通道。

公开(公告)号：US20130273589A1

公开(公告)日：2013-10-17

申请号：US13911490

申请日：2013-06-06

Applicant: Corning Incorporated

Inventor： Ronald Allen Faris ,  Vasiliy Nikolaevich Goral ,  Miya Yi-Cheng Hsieh ,  Odessa Natalie Petzold ,  Po Ki Yuen

IPC: G01N33/50

CPC classification number: G01N33/5008 ,  C12M21/08 ,  C12M23/16 ,  C12M23/24 ,  C12M23/40 ,  C12M29/10

Abstract: A microfluidic cell culture apparatus includes a cell retention chamber and a perfusion channel. The cell retention chamber has a structured surface. The structured surface includes a major surface from which a plurality of projections extends into the chamber. The plurality of projections are arranged to suspend cells cultured in the chamber above the major surface. The first perfusion channel is configured to provide laminar flow of a fluid through the channel and forms a plurality of openings in communication with the cell retention chamber. The openings are configured to prevent cells from the retention chamber from entering the perfusion channel.

Abstract translation: 微流体细胞培养装置包括细胞保留室和灌注通道。 细胞保留室具有结构化表面。 结构化表面包括主表面，多个突起从该主表面延伸到腔室中。 多个突起被布置成悬浮在主表面上方的室中培养的细胞。 第一灌注通道构造成提供流体通过通道的层流，并形成与细胞保持室连通的多个开口。 开口构造成防止来自保留腔的细胞进入灌注通道。