公开(公告)号：US20030008308A1

公开(公告)日：2003-01-09

申请号：US10118466

申请日：2002-04-05

Applicant: California Institute of Technology

Inventor： Markus M. Enzelberger ,  Carl L. Hansen ,  Jian Liu ,  Stephen R. Quake ,  Chiem Ma

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  C12M001/34

CPC classification number: C12Q1/68 ,  B01F5/102 ,  B01F5/108 ,  B01F13/0059 ,  B01F15/06 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/525 ,  B01L9/527 ,  B01L2300/0861 ,  B01L2300/088 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6844 ,  C12Q3/00 ,  C12Q2565/629

Abstract: The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certaom devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.

Abstract translation: 本发明提供了在各种类型的热循环反应中使用该微流体装置和方法的微流体装置和方法。 Certaom设备包括旋转微流体通道和沿着旋转微流体通道的不同位置处的多个温度区域，在该温度区域调节温度。 溶液可以重复通过温度区域，使得溶液暴露于不同的温度。 其他微流体装置包括通过垂直和水平流动通道相交形成的反应室阵列，具有调节反应室温度的能力。 微流体装置可用于进行许多不同的分析，包括各种引物延伸反应和核酸扩增反应。