公开(公告)号：US20020109114A1

公开(公告)日：2002-08-15

申请号：US10045132

申请日：2001-10-23

Applicant: California Institute of Technology

Inventor： B. Scott Driggs ,  Markus M. Enzelberger ,  Stephen R. Quake

IPC: F16K031/02 ,  C23F001/00

CPC classification number: F16K31/02 ,  B33Y80/00 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0011 ,  F16K99/0051 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008

Abstract: Valve structures formed in elastomer material are electrostatically actuated by applying voltage to a flexible, electrically conductive wire pattern. An actuation force generated between the patterned wire structure and an electrode result in closure of a flow channel formed in elastomer material underlying the wire. In one embodiment of a valve structure in accordance with the present invention, the wire structure is patterned by lithography and etching of a copper/polyimide laminate, with an underlying gold plate positioned on the opposite side of the flow channel serving as an electrode. In an alternative embodiment, a first wire structure is patterned by physically cutting out a first pattern of strips from an Aluminum/Mylar laminate sheet. A second patterned wire structure serving as the electrode is formed by the same method, and positioned on the opposite side of a control channel. Application of an actuation force between the first and second patterned strips closes the control channel and an associated flow channel underlying the control channel.

Abstract translation: 弹性体材料中形成的阀结构通过将电压施加到柔性导电的导线图案而被静电致动。 在图案化的线结构和电极之间产生的致动力导致在线下方的弹性体材料中形成的流动通道的封闭。 在根据本发明的阀结构的一个实施例中，通过光刻和铜/聚酰亚胺层压板的蚀刻来对线结构进行图案化，其中下面的金板位于用作电极的流动通道的相对侧上。 在替代实施例中，通过从铝/聚酯薄膜层压板物理切割第一图案条，对第一线结构图案化。 用作电极的第二图案化线结构通过相同的方法形成，并且位于控制通道的相对侧上。 在第一和第二图案化带之间的致动力的施加关闭控制通道和控制通道下方的相关联的流动通道。

公开(公告)号：US20030008308A1

公开(公告)日：2003-01-09

申请号：US10118466

申请日：2002-04-05

Applicant: California Institute of Technology

Inventor： Markus M. Enzelberger ,  Carl L. Hansen ,  Jian Liu ,  Stephen R. Quake ,  Chiem Ma

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  C12M001/34

CPC classification number: C12Q1/68 ,  B01F5/102 ,  B01F5/108 ,  B01F13/0059 ,  B01F15/06 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/525 ,  B01L9/527 ,  B01L2300/0861 ,  B01L2300/088 ,  B01L2300/0883 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6844 ,  C12Q3/00 ,  C12Q2565/629

Abstract: The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certaom devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.

Abstract translation: 本发明提供了在各种类型的热循环反应中使用该微流体装置和方法的微流体装置和方法。 Certaom设备包括旋转微流体通道和沿着旋转微流体通道的不同位置处的多个温度区域，在该温度区域调节温度。 溶液可以重复通过温度区域，使得溶液暴露于不同的温度。 其他微流体装置包括通过垂直和水平流动通道相交形成的反应室阵列，具有调节反应室温度的能力。 微流体装置可用于进行许多不同的分析，包括各种引物延伸反应和核酸扩增反应。