公开(公告)号：US20140127777A1

公开(公告)日：2014-05-08

申请号：US14066371

申请日：2013-10-29

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： David A. Tirrell ,  Yi Tang

IPC: C12N9/96 ,  C07K2/00

CPC classification number: C12N9/96 ,  C07K1/107 ,  C07K2/00 ,  C07K14/47 ,  C07K14/4705 ,  C12P21/02

Abstract: The present invention provides a method for producing modified stable polypeptides introducing at least one non-natural amino acid into the hydrophobic region of the polypeptide. The thermal and chemical stability of such polypeptides is improved compared to those properties of its corresponding wild type proteins.The invention further provides purified leucine zipper and coiled-coil proteins in which the leucine residues have been replaced with 5,5,5-trifluoroleucines, and the modified proteins so produced demonstrate increased thermal and chemical stability compared to their corresponding wild-type natural proteins.

Abstract translation: 本发明提供了一种制备将至少一个非天然氨基酸引入多肽疏水区的修饰稳定多肽的方法。 与其相应的野生型蛋白质的那些性质相比，这些多肽的热和化学稳定性得到改善。 本发明还提供纯化的亮氨酸拉链和卷曲螺旋蛋白，其中亮氨酸残基已经被5,5,5-三氟色氨酸替代，并且与其相应的野生型天然蛋白相比，所产生的修饰的蛋白质表现出增加的热和化学稳定性 。

公开(公告)号：US08980581B2

公开(公告)日：2015-03-17

申请号：US13969368

申请日：2013-08-16

Applicant: California Institute of Technology

Inventor： Pin Wang ,  Inchan Kwon ,  Soojin Son ,  Yi Tang ,  David Tirrell

IPC: C12P21/02 ,  G01N33/68 ,  C12N9/00 ,  C12N15/67 ,  C12N15/70

CPC classification number: C12N9/93 ,  C12N15/67 ,  C12N15/70

Abstract: The invention provides certain embodiments relating to methods and compositions for incorporating non-natural amino acids into a polypeptide or protein by utilizing a mutant or modified aminoacyl-tRNA synthetase to charge the non-natural amino acid to a the corresponding tRNA. In certain embodiments, the tRNA is also modified such that the complex forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with unmodified tRNA/aminoacyl-tRNA synthetase pairs.

Abstract translation: 本发明提供了涉及将非天然氨基酸并入多肽或蛋白质的方法和组合物的某些实施方案，所述方法和组合物通过利用突变或修饰的氨酰-tRNA合成酶将非天然氨基酸加载至相应的tRNA。 在某些实施方案中，tRNA也被修饰，使得复合物与通常与未修饰的tRNA /氨酰-tRNA合成酶对形成摆动碱基配对的密码子形成严格的沃森 - 克里克碱基配对。

公开(公告)号：US20140045261A1

公开(公告)日：2014-02-13

申请号：US13969368

申请日：2013-08-16

Applicant: California Institute of Technology

Inventor： Pin Wang ,  Inchan Kwon ,  Soojin Son ,  Yi Tang ,  David Tirrell

IPC: C12N9/00 ,  C12N15/70

CPC classification number: C12N9/93 ,  C12N15/67 ,  C12N15/70

Abstract: The invention provides certain embodiments relating to methods and compositions for incorporating non-natural amino acids into a polypeptide or protein by utilizing a mutant or modified aminoacyl-tRNA synthetase to charge the non-natural amino acid to a the corresponding tRNA. In certain embodiments, the tRNA is also modified such that the complex forms strict Watson-Crick base-pairing with a codon that normally forms wobble base-pairing with unmodified tRNA/aminoacyl-tRNA synthetase pairs.

Abstract translation: 本发明提供了涉及将非天然氨基酸并入多肽或蛋白质的方法和组合物的某些实施方案，所述方法和组合物通过利用突变或修饰的氨酰-tRNA合成酶将非天然氨基酸加载至相应的tRNA。 在某些实施方案中，tRNA也被修饰，使得复合物与通常与未修饰的tRNA /氨酰-tRNA合成酶对形成摆动碱基配对的密码子形成严格的沃森 - 克里克碱基配对。