公开(公告)号：US20030152969A1

公开(公告)日：2003-08-14

申请号：US10289141

申请日：2002-11-05

Applicant: California Institute of Technology

Inventor： Stephen R. Quake ,  R. Michael van Dam ,  James P. Brody ,  Rebecca Shafee

IPC: C12Q001/68 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G06F19/20 ,  G06F17/15 ,  G06F19/24

Abstract: Techniques for processing gene expression data and predicting gene relationships are provided. More specifically, a method for processing gene expression ratios may include discretizing a first expression ratio for a first gene and a second expression ratio for a second gene for each of a plurality of experiments into one of three indications, calculating the probability that the combination of the discretized expression ratios for each of the plurality of experiments arises by random chance, and determining whether the first gene and the second gene are related.

Abstract translation: 提供了处理基因表达数据和预测基因关系的技术。 更具体地，用于处理基因表达比率的方法可以包括将用于第一基因的第一表达比例和第二基因的第二表达比例分离为多个实验中的每一个进行三个指示之一，计算组合 多个实验中的每一个的离散化表达比均由随机机会产生，并且确定第一基因和第二基因是否相关。

公开(公告)号：US20030148539A1

公开(公告)日：2003-08-07

申请号：US10288248

申请日：2002-11-04

Applicant: California Institute of Technology

Inventor： R. Michael van Dam ,  Stephen R. Quake ,  Axel Scherer ,  Matthew O. Reese

IPC: B01L003/02 ,  G01N001/10

CPC classification number: B01L3/0244 ,  B01L3/0248 ,  B01L3/0275 ,  B01L3/5085 ,  B01L2200/0642 ,  B01L2300/0838 ,  B01L2300/123 ,  G01N2035/1037 ,  Y10T436/2575

Abstract: A fluid dispensing system for at least biological applications, e.g., oligonucleotides, peptide nucleic acids (nullPNAnull), proteins, polysaccharides, polypeptides, inorganic solutions, microelectromechanical systems (MEMS), optical sensors, and other applications. The dispensing system includes a fluid dispensing apparatus for applying selected fluids in a predetermined manner to form a plurality of spots based upon one or more of the selected fluids on a surface of a substrate. The apparatus comprises an elongated member having at least a tip portion, which extends from the elongated member. The apparatus also has an etched trench extending along a portion of a length of the elongated member to the tip to form an opening defined on the tip portion and coupled to the etched trench. A flexible region is defined within the elongated member to allow the tip to adjust in position upon contact with the surface of the substrate. A fluid is disposed within the etched trench. The fluid is output through the opening on the tip to form more than one spots on the surface of the substrate.

Abstract translation: 用于至少生物应用的流体分配系统，例如寡核苷酸，肽核酸（“PNA”），蛋白质，多糖，多肽，无机溶液，微机电系统（MEMS），光学传感器和其它应用。 分配系统包括用于以预定方式施加选定流体以形成基于基底表面上的一种或多种所选流体的多个斑点的流体分配装置。 该装置包括细长构件，该细长构件至少具有从细长构件延伸的尖端部分。 该设备还具有沿着细长构件的长度的一部分延伸到尖端的蚀刻沟槽，以形成限定在尖端部分上并连接到蚀刻沟槽的开口。 柔性区域限定在细长构件内，以允许尖端在与基底的表面接触时调节位置。 流体被设置在蚀刻沟槽内。 流体通过尖端上的开口输出，以在基底表面上形成多于一个点。