公开(公告)号：US10040820B2

公开(公告)日：2018-08-07

申请号：US13378808

申请日：2010-06-21

Applicant: Camilo Colaco ,  Colin Richard Bignell

Inventor： Camilo Colaco ,  Colin Richard Bignell

IPC: C07K1/18 ,  A61K39/00 ,  A61K39/04 ,  A61K39/09 ,  A61K39/095 ,  A61K39/12 ,  A61K39/145 ,  A61K39/29 ,  A61K39/385 ,  A61K39/39 ,  C07K14/195

CPC classification number: C07K1/18 ,  A61K39/0011 ,  A61K39/04 ,  A61K39/092 ,  A61K39/095 ,  A61K39/12 ,  A61K39/145 ,  A61K39/29 ,  A61K39/385 ,  A61K39/39 ,  A61K2039/55516 ,  A61K2039/605 ,  C07K14/195 ,  C12N2710/14043 ,  C12N2760/16134 ,  C12N2770/24234 ,  Y02A50/394 ,  Y02A50/397 ,  Y02A50/403 ,  Y02A50/464 ,  Y02A50/466 ,  Y02A50/478

Abstract: The present invention provides an improved method for the purification of a mixture of complexes comprising a stress protein complexed to a peptide or peptide fragment from a source mixture, typically a cell lysate. The method of the invention provides for protein complexes to be purified using ion exchange chromatography based methods, wherein a modified buffer solution is used which results in the purified stress protein complexes being more immunogenic than protein complexes obtained using conventional methodology. The purified complexes can be used to produce improved vaccine preparations which elicit enhanced immune responses in the subjects to whom the vaccine compositions are administered.

公开(公告)号：US20120135029A1

公开(公告)日：2012-05-31

申请号：US13378808

申请日：2010-06-21

Applicant: Camilo Colaco ,  Colin Richard Bignell

Inventor： Camilo Colaco ,  Colin Richard Bignell

IPC: A61K39/385 ,  C09K3/00 ,  A61P37/04 ,  C07K1/18

CPC classification number: C07K1/18 ,  A61K39/0011 ,  A61K39/04 ,  A61K39/092 ,  A61K39/095 ,  A61K39/12 ,  A61K39/145 ,  A61K39/29 ,  A61K39/385 ,  A61K39/39 ,  A61K2039/55516 ,  A61K2039/605 ,  C07K14/195 ,  C12N2710/14043 ,  C12N2760/16134 ,  C12N2770/24234 ,  Y02A50/394 ,  Y02A50/397 ,  Y02A50/403 ,  Y02A50/464 ,  Y02A50/466 ,  Y02A50/478

Abstract: The present invention provides an improved method for the purification of a mixture of complexes comprising a stress protein complexed to a peptide or peptide fragment from a source mixture, typically a cell lysate. The method of the invention provides for protein complexes to be purified using ion exchange chromatography based methods, wherein a modified buffer solution is used which results in the purified stress protein complexes being more immunogenic than protein complexes obtained using conventional methodology. The purified complexes can be used to produce improved vaccine preparations which elicit enhanced immune responses in the subjects to whom the vaccine compositions are administered.

Abstract translation: 本发明提供了一种用于纯化复合物的混合物的改进方法，其包括与源混合物（通常为细胞裂解物）与肽或肽片段复合的应激蛋白。 本发明的方法提供使用基于离子交换层析的方法纯化的蛋白质复合物，其中使用修饰的缓冲溶液，其导致纯化的应激蛋白复合物比使用常规方法获得的蛋白质复合物更具免疫原性。 纯化的复合物可用于产生改进的疫苗制剂，其在施用疫苗组合物的受试者中引起增强的免疫应答。