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    METHODS AND SYSTEMS FOR CONTINUOUS FLOW CELL LYSIS IN A MICROFLUIDIC DEVICE
    1.
    发明申请
    METHODS AND SYSTEMS FOR CONTINUOUS FLOW CELL LYSIS IN A MICROFLUIDIC DEVICE 审中-公开
    微流控器件中连续流量池的方法与系统

    公开(公告)号：US20150184127A1

    公开(公告)日：2015-07-02

    申请号：US14586576

    申请日：2014-12-30

    申请人： Canon U.S. Life Sciences, Inc.

    发明人： Ian M. White ,  Jeffrey Burke ,  Kunal Pandit

    IPC分类号： C12N5/078 ,  B32B37/00 ,  C12M1/00 ,  C12N1/20 ,  C12N1/06

    CPC分类号： B32B37/0076 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2400/086 ,  C12M47/06 ,  C12N1/066

    摘要： The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow mechanical cell lysis. In one embodiment, a microfluidic device includes one or more microfluidic channels, each channel comprising constricted regions and non-constricted regions separating the constricted regions, wherein the constricted regions are configured to disrupt the cellular membranes of cells in fluid flowing through the one or more microfluidic channels.

    摘要翻译： 本发明涉及微流控装置中细胞裂解的方法和系统。 更具体地，本发明的实施方案涉及用于快速连续流动机械细胞裂解的方法和系统。 在一个实施例中，微流体装置包括一个或多个微流体通道，每个通道包括收缩区域和分离收缩区域的非收缩区域，其中缩小区域被配置为破坏流过该一个或多个流体的流体中的细胞的细胞膜 微流体通道。

    Methods and systems for continuous flow cell lysis in a microfluidic device
    2.
    发明授权
    Methods and systems for continuous flow cell lysis in a microfluidic device 有权

    公开(公告)号：US10137673B2

    公开(公告)日：2018-11-27

    申请号：US14586576

    申请日：2014-12-30

    申请人： Canon U.S. Life Sciences, Inc.

    发明人： Ian M. White ,  Jeffrey Burke ,  Kunal Pandit

    IPC分类号： B32B37/00 ,  C12N1/06 ,  C12M1/00 ,  B01L3/00

    摘要： The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow mechanical cell lysis. In one embodiment, a microfluidic device includes one or more microfluidic channels, each channel comprising constricted regions and non-constricted regions separating the constricted regions, wherein the constricted regions are configured to disrupt the cellular membranes of cells in fluid flowing through the one or more microfluidic channels.

    METHODS, DEVICES AND SYSTEMS FOR EMULSION/DROPLET PCR
    4.
    发明申请
    METHODS, DEVICES AND SYSTEMS FOR EMULSION/DROPLET PCR 审中-公开
    方法，用于乳液/滴定PCR的装置和系统

    公开(公告)号：US20150184151A1

    公开(公告)日：2015-07-02

    申请号：US14586564

    申请日：2014-12-30

    申请人： Canon U.S. Life Sciences, Inc.

    发明人： Ian M. White ,  Kunal Pandit ,  Srinivasa Raghavan

    IPC分类号： C12N15/10 ,  G01N13/02 ,  C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12N15/1075 ,  C12Q1/6844 ,  G01N13/02 ,  G01N2013/0241 ,  C12Q2527/125 ,  C12Q2563/159

    摘要： The present invention relates generally to the use of a class of surfactants for emulsion and droplet polymerase chain reaction (“PCR”) mixtures. The class of surfactants consists of those having the chemical formula R—(OCH2CH2)n—OH, wherein R is an alkyl group consisting of 12 to 18 carbons and n is 2 to 25. The present invention also relates to methods, devices, systems, and kits incorporating the above-described class of surfactants.

    摘要翻译： 本发明一般涉及一类用于乳液和液滴聚合酶链反应（“PCR”）混合物的表面活性剂的用途。 表面活性剂类由具有化学式R-（OCH 2 CH 2）n -OH的化合物组成，其中R是由12至18个碳组成的烷基，n是2至25.本发明还涉及方法，装置，系统 以及并入上述类型的表面活性剂的试剂盒。