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    Composition for cytocompatible, injectable, self-gelling chitosan solutions for encapsulating and delivering live cells or biologically active factors
    1.
    发明申请
    Composition for cytocompatible, injectable, self-gelling chitosan solutions for encapsulating and delivering live cells or biologically active factors 审中-公开
    用于包封和递送活细胞或生物活性因子的细胞相容性，可注射的自凝胶壳聚糖溶液的组成

    公开(公告)号：US20060127873A1

    公开(公告)日：2006-06-15

    申请号：US10521524

    申请日：2003-07-16

    申请人： Caroline Hoemann ,  Abdellatif Chenite ,  Michael Buschmann ,  Alessio Sarreqi ,  Jun Sun

    发明人： Caroline Hoemann ,  Abdellatif Chenite ,  Michael Buschmann ,  Alessio Sarreqi ,  Jun Sun

    IPC分类号： A01N1/02 ,  C12N5/06 ,  A61K31/717

    CPC分类号： A61L27/3604 ,  A01N1/0205 ,  A01N1/021 ,  A01N1/0231 ,  A61K9/0024 ,  A61K31/717 ,  A61L27/20 ,  A61L27/3641 ,  A61L27/365 ,  A61L27/3654 ,  A61L27/3839 ,  A61L27/3847 ,  A61L27/3852 ,  A61L27/50 ,  A61L27/52 ,  C08L5/08

    摘要： The present invention provides compositions and methods for tissue repair using a cytocompatible self-gelling cross-linked hydrogel. The composition comprises a biocompatible mixture of chitosan, bifunctional dialdehyde, and hydroxylated polymer, which can be used to immobilize or encapsulate viable cells, or bioactive substances. The method includes the process of mixing bioactive substances, live cells, and/or extracellular matrix components with a cross-linking solution comprising a bifunctional aldehyde-treated hydroxylated polymer such as hydroxyethyl cellulose. The cross-linking solution is then mixed homogenously with a neutral isotonic chitosan solution. The chitosan becomes cross-linked by the bifunctional aldehyde, while the cells are protected from potentially nocive effects of the aldehyde cross-linker by the hydroxylated polymer. The injectable solution retains cell viability and bioactivity, and immobilizes cells at the site of injection or delivery. Depending on the particular application, mixtures of chitosan and bifunctional dialdehyde may be employed. The injectable solution also liberates bioactive substances with controlled release kinetics from the site of injection.

    摘要翻译： 本发明提供使用细胞相容性自凝胶交联水凝胶进行组织修复的组合物和方法。 该组合物包含壳聚糖，双功能二醛和羟基化聚合物的生物相容性混合物，其可用于固定或包囊活细胞或生物活性物质。 该方法包括将生物活性物质，活细胞和/或细胞外基质组分与包含双官能醛处理的羟基化聚合物如羟乙基纤维素的交联溶液混合的方法。 然后将交联溶液与中性等渗壳聚糖溶液均匀混合。 壳聚糖通过双官能醛交联，同时保护细胞免受羟基化聚合物对醛交联剂的潜在的伤害作用。 注射溶液保留细胞活力和生物活性，并将细胞固定在注射或递送部位。 根据具体应用，可以使用壳聚糖和双官能二醛的混合物。 注射溶液也释放出具有从注射部位释放动力学的生物活性物质。