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1.发明授权Method for inhibiting chymopapain and papain enzyme activity with polysaccharides of animal origin 失效用动物源多糖抑制木瓜凝乳蛋白酶和木瓜蛋白酶活性的方法
公开(公告)号：US5670358A
公开(公告)日：1997-09-23
申请号：US545439
申请日：1995-10-19
申请人： Catherine T. Lee , Cynthia Zerfass , Tan Thanh Dinh , Minh T. Ma
发明人： Catherine T. Lee , Cynthia Zerfass , Tan Thanh Dinh , Minh T. Ma
CPC分类号： C12N5/0676 , C12N5/067 , C12N9/99 , C12N2501/905 , C12N2501/91 , C12N2509/00
摘要： The activities of chymopapain or papain in enzyme mixtures are inhibited by the addition of glycogen, hyaluronic acid, or desulfated heparin. The process for isolating viable hepatocytes or pancreatic cells by treatment with papain or chymopapain is improved by terminating the treatment by inhibiting the reaction with these polysaccharides. Similarly, the process for removing undesired tissue by treatment with papain or chymopapain is improved by terminating the treatment by inhibiting the reaction with these polysaccharides.
摘要翻译： 木瓜凝乳蛋白酶或木瓜蛋白酶在酶混合物中的活性被加入糖原，透明质酸或脱硫酸肝素所抑制。 通过用木瓜蛋白酶或木瓜凝乳蛋白酶处理分离活肝细胞或胰腺细胞的过程通过终止通过抑制与这些多糖的反应的处理来改善。 类似地，通过用木瓜蛋白酶或木瓜凝乳蛋白酶处理去除不需要的组织的方法通过终止通过抑制与这些多糖的反应的处理来改善。
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2.发明授权公开(公告)号：US06783988B1
公开(公告)日：2004-08-31
申请号：US09693186
申请日：2000-10-19
IPC分类号： G01N3392
摘要： A highly sensitive and specific method for the detection and quantification of lipids is provided. Specifically, methods for the simultaneous detection and quantification of phospholipids extracted from mammalian tissues is described. The analytical methods provided disclose a modified one-dimensional thin-layer chromatography technique specifically developed to rapidly and accurantely detect and quantify phospholipids from mammalian cardiac tissues.
摘要翻译： 提供了一种用于检测和定量脂质的高灵敏度和具体方法。 具体地说，描述了从哺乳动物组织中提取的同时检测和定量磷脂的方法。 所提供的分析方法公开了专门开发用于快速且精确地检测和定量来自哺乳动物心脏组织的磷脂的修饰的一维薄层色谱技术。
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3.发明授权
公开(公告)号：US6008292A
公开(公告)日：1999-12-28
申请号：US982846
申请日：1997-12-02
CPC分类号： A61L27/3695 , A61L27/24 , A61L27/3604 , A61L27/3683 , A61L27/3687 , A61F2310/00365 , A61L2400/02 , A61L2430/40
摘要： A method for preparing collagenous biological tissues for use as implantable bioprosthetic material, and articles prepared thereby. The method generally comprises the steps of a) contacting the tissue with an aldehyde fixative to effect cross-linking of the connective tissue protein(s) and subsequently b) contacting the tissue with a polyglycidyl ether. The treatment of the crosslinked tissue with the polyglycidyl ether serves to mitigate the tissue's propensity to undergo calcification following implantation in the body.
摘要翻译： 一种用于制备用作可植入生物假体材料的胶原生物组织的方法，以及由此制备的制品。 该方法通常包括以下步骤：a）使组织与醛固定剂接触以实现结缔组织蛋白的交联，随后b）使组织与聚缩水甘油醚接触。 用聚缩水甘油醚处理交联组织用于减轻组织在体内植入后经历钙化的倾向。
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