公开(公告)号：US20130022626A1

公开(公告)日：2013-01-24

申请号：US13638860

申请日：2011-04-04

申请人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Soo Young Kwak ,  Mi Ji Lee ,  In Young Choi ,  Se Chang Kwon

发明人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Soo Young Kwak ,  Mi Ji Lee ,  In Young Choi ,  Se Chang Kwon

IPC分类号： C07K19/00 ,  A61P15/00 ,  A61P15/08 ,  C07K1/107 ,  A61K39/395

CPC分类号： A61K47/48215 ,  A61K47/60 ,  A61K47/68

摘要： The present invention relates to a long-acting human follicle-stimulating hormone formulation having improved in vivo duration and stability, comprising a human follicle-stimulating hormone conjugate that is prepared by covalently linking human follicle-stimulating hormone with an immunoglobulin Fc region via a non-peptidyl polymer, and a preparation method thereof. The long-acting human follicle-stimulating hormone formulation of the present invention maintains in vivo activity of human follicle-stimulating hormone at a relatively high level and remarkably increases the serum half-life thereof.

摘要翻译： 本发明涉及具有改善的体内持续时间和稳定性的长效促卵泡激素制剂，其包含人促卵泡激素缀合物，其通过非激素共价连接人卵泡刺激激素与免疫球蛋白Fc区而制备 - 肽基聚合物及其制备方法。 本发明的长效人促卵泡激素制剂在较高水平维持人卵泡刺激激素的体内活性，并且显着增加其血清半衰期。

公开(公告)号：US09186415B2

公开(公告)日：2015-11-17

申请号：US13638860

申请日：2011-04-04

申请人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Soo Young Kwak ,  Mi Ji Lee ,  In Young Choi ,  Se Chang Kwon

发明人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Soo Young Kwak ,  Mi Ji Lee ,  In Young Choi ,  Se Chang Kwon

IPC分类号： A61K38/24 ,  A61K38/00 ,  A61K38/16 ,  C07K16/00 ,  C07K1/00 ,  A61K47/48

CPC分类号： A61K47/48215 ,  A61K47/60 ,  A61K47/68

摘要： The present invention relates to a long-acting human follicle-stimulating hormone formulation having improved in vivo duration and stability, comprising a human follicle-stimulating hormone conjugate that is prepared by covalently linking human follicle-stimulating hormone with an immunoglobulin Fc region via a non-peptidyl polymer, and a preparation method thereof. The long-acting human follicle-stimulating hormone formulation of the present invention maintains in vivo activity of human follicle-stimulating hormone at a relatively high level and remarkably increases the serum half-life thereof.

摘要翻译： 本发明涉及具有改善的体内持续时间和稳定性的长效促卵泡激素制剂，其包含人促卵泡激素缀合物，其通过非激素共价连接人卵泡刺激激素与免疫球蛋白Fc区而制备 - 肽基聚合物及其制备方法。 本发明的长效人促卵泡激素制剂在较高水平维持人卵泡刺激激素的体内活性，并且显着增加其血清半衰期。

公开(公告)号：US09243237B2

公开(公告)日：2016-01-26

申请号：US13881974

申请日：2011-10-26

申请人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Sung Hwan Hong ,  Se Chang Kwon

发明人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Sung Hwan Hong ,  Se Chang Kwon

IPC分类号： A61K38/00 ,  C12N9/64 ,  C07K14/745

CPC分类号： C12N9/6437 ,  C07K14/745 ,  C12N15/85 ,  C12N2830/85

摘要： A method for the mass production of human coagulation Factor VII. The method includes a) providing an expression vector carrying i) a dihydrofolate reductase promoter devoid of one or more CCGCC repeat sequences from the GC-rich region thereof and a dihydrofolate reductase (DHFR) gene operably linked thereto and ii) a cytomegalovirus (CMV) promoter and a human coagulation Factor VII gene operably linked thereto; b) obtaining a transformed host cell line containing the expression vector; and c) culturing the transfected host cell in the presence of a dihydrofolate reductase inhibitor to select cells which express human coagulation Factor VII with high efficiency; and d) adding sodium butyrate to the selected host cells.

摘要翻译： 大量生产人凝血因子VII的方法。 所述方法包括：a）提供表达载体，其携带i）没有来自其富含GC的区域的一个或多个CCGCC重复序列的二氢叶酸还原酶启动子和与其可操作地连接的二氢叶酸还原酶（DHFR）基因，和ii）巨细胞病毒（CMV） 启动子和与之有效连接的人凝血因子VII基因; b）获得含有表达载体的转化的宿主细胞系; 和c）在二氢叶酸还原酶抑制剂存在下培养转染的宿主细胞以选择以高效率表达人凝血因子VII的细胞; 和d）向所选择的宿主细胞中加入丁酸钠。

公开(公告)号：US20130273634A1

公开(公告)日：2013-10-17

申请号：US13881974

申请日：2011-10-26

申请人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Sung Hwan Hong ,  Se Chang Kwon

发明人： Chang Hwan Kim ,  Sung Kap Hong ,  Byung Sun Lee ,  Sung Hwan Hong ,  Se Chang Kwon

IPC分类号： C12N9/64

CPC分类号： C12N9/6437 ,  C07K14/745 ,  C12N15/85 ,  C12N2830/85

摘要： A method for the mass production of human coagulation factor VII. The method includes a) providing an expression vector carrying i) a dihydrofolate reductase promoter devoid of one or more CCGCC repeat sequences from the GC-rich region thereof and a dihydrofolate reductase (DHFR) gene operably linked thereto and ii) a cytomegalovirus (CMV) promoter and a human coagulation factor VII gene operably linked thereto; b) obtaining a transformed a host cell line containing the expression vector; and c) culturing the transfected host cell in the presence of a dihydrofolate reductase inhibitor to select cells which express human coagulation factor VII with high efficiency; and d) adding sodium butyrate to the selected host cells.

摘要翻译： 大量生产人凝血因子VII的方法。 所述方法包括：a）提供表达载体，其携带i）没有来自其富含GC的区域的一个或多个CCGCC重复序列的二氢叶酸还原酶启动子和与其可操作地连接的二氢叶酸还原酶（DHFR）基因，和ii）巨细胞病毒（CMV） 启动子和与之有效连接的人凝血因子VII基因; b）获得含有表达载体的转化的宿主细胞系; 和c）在二氢叶酸还原酶抑制剂存在下培养转染的宿主细胞以选择以高效率表达人凝血因子VII的细胞; 和d）向所选择的宿主细胞中加入丁酸钠。

公开(公告)号：US20130095090A1

公开(公告)日：2013-04-18

申请号：US13807572

申请日：2011-06-30

申请人： Dae Hae Song ,  Sung In Lim ,  Chang Hwan Kim ,  Sung Kap Hong ,  Dae Seong Im ,  Se Chang Kwon

发明人： Dae Hae Song ,  Sung In Lim ,  Chang Hwan Kim ,  Sung Kap Hong ,  Dae Seong Im ,  Se Chang Kwon

IPC分类号： C12N9/96

CPC分类号： C12N9/96 ,  A61K9/0019 ,  A61K47/68 ,  A61K47/6811 ,  A61K47/6889 ,  C07K14/745 ,  C07K2319/30

摘要： Disclosed are a blood coagulation factor complex in which FacVIIa, a non-peptidyl polymer and an immunoglobulin Fc region are bonded by covalent bonds, and the uses thereof. The FacVIIa complex guarantees the in vivo activity of FacVIIa and significantly enhances the serum half life of FacVIIa, so that it is useful for developing long-acting FacVIIa formulations which can improve the compliance of role behavior of patients whose blood does not coagulate.

摘要翻译： 公开了其中FacVIIa，非肽基聚合物和免疫球蛋白Fc区通过共价键键合的凝血因子复合物及其用途。 FacVIIa复合物保证FacVIIa的体内活性，并显着增强FacVIIa的血清半衰期，因此它可用于开发长效FacVIIa制剂，可以改善血液不凝血的患者的作用行为的顺应性。