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    Ligand analog-irreversible enzyme inhibitor conjugates and methods for use
    4.
    发明授权
    Ligand analog-irreversible enzyme inhibitor conjugates and methods for use 失效
    配体类似物不可逆酶抑制剂缀合物及其使用方法

    公开(公告)号：US4273866A

    公开(公告)日：1981-06-16

    申请号：US9007

    申请日：1979-02-05

    申请人： Houston F. Voss ,  Jacob Plattner ,  Thomas R. Herrin

    发明人： Houston F. Voss ,  Jacob Plattner ,  Thomas R. Herrin

    IPC分类号： C07F9/40 ,  C12Q1/46 ,  G01N33/532 ,  G01N33/542 ,  G01N33/54 ,  C12N9/99

    CPC分类号： G01N33/542 ,  C07F9/4075 ,  G01N33/532 ,  Y10S435/81 ,  Y10S435/962 ,  Y10S436/825

    摘要： The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction.The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.

    摘要翻译： 本发明包括用于测定测试样品中配体的方法，包括与测试样品混合配体类似物不可逆酶抑制剂缀合物和可与该配体结合的结合蛋白和配体类似物不可逆酶抑制剂缀合物，其中配体类似物 由结合蛋白结合的可逆酶抑制剂缀合物与测试样品中配体的量相关，所述结合蛋白在结合到缀合物的配体类似物部分时使不可逆酶抑制剂失活; 混合由结合蛋白未结合的配体模拟不可逆酶抑制剂缀合物不可逆地抑制的酶; 并将底物与酶混合并监测酶底物反应。 本发明还包括在实施该方法中用作试剂的配体类似物不可逆酶抑制剂缀合物。 本发明的方法和试剂特别可用于确定生物流体中的药物，激素等。

    Ligand analog-irreversible enzyme inhibitor conjugates
    9.
    发明授权
    Ligand analog-irreversible enzyme inhibitor conjugates 失效
    配体类似物不可逆酶抑制剂缀合物

    公开(公告)号：US4550163A

    公开(公告)日：1985-10-29

    申请号：US228414

    申请日：1981-01-26

    申请人： Houston F. Voss ,  Jacob Plattner ,  Thomas R. Herrin

    发明人： Houston F. Voss ,  Jacob Plattner ,  Thomas R. Herrin

    IPC分类号： C07F9/40 ,  G01N33/532 ,  G01N33/542 ,  C07F9/60

    CPC分类号： G01N33/542 ,  C07F9/4075 ,  G01N33/532

    摘要： The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction.The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.

    摘要翻译： 本发明包括用于测定测试样品中配体的方法，包括与测试样品混合配体类似物不可逆酶抑制剂缀合物和可与该配体结合的结合蛋白和配体类似物不可逆酶抑制剂缀合物，其中配体类似物 由结合蛋白结合的可逆酶抑制剂缀合物与测试样品中配体的量相关，所述结合蛋白在结合到缀合物的配体类似物部分时使不可逆酶抑制剂失活; 混合由结合蛋白未结合的配体模拟不可逆酶抑制剂缀合物不可逆地抑制的酶; 并将底物与酶混合并监测酶底物反应。 本发明还包括在实施该方法中用作试剂的配体类似物不可逆酶抑制剂缀合物。 本发明的方法和试剂特别可用于确定生物流体中的药物，激素等。