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公开(公告)号：US5348742A
公开(公告)日：1994-09-20
申请号：US46464
申请日：1993-04-13
CPC分类号： C12R1/39 , A01N63/00 , C12N15/52 , Y10S435/822 , Y10S435/876
摘要： Purified bacterial strains that are effective for the inhibition of plant pathogens, including the fungi Rhizoctonia solani and Pythium ultimum have been isolated. These strains are useful as biocontrol agents, and can be used to produce antifungal metabolites, such as antibiotic compounds, active against the plant pathogenic fungi Rhizoctonia solani and Pythium ultimum. Both the purified bacterial strains and the antibiotic compounds can be used as active agents for biocontrol compositions.
摘要翻译： 已经分离了对抑制植物病原体有效的纯化菌株，包括真菌丝核菌（Rhizoctonia solani）和腐霉菌（Pythium ultimum）。 这些菌株可用作生物防治剂，可用于生产抗真菌代谢物，如抗生素化合物，对植物致病真菌丝核菌（Rhizoctonia solani）和腐霉菌（Pythium ultimum）有活性。 纯化的细菌菌株和抗生素化合物均可用作生物控制组合物的活性剂。
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2.发明授权Genomic DNA encoding a Pseudomonas global transcriptional activation element and its use in activating gene expression 失效编码假单胞菌全基因转录激活元件的基因组DNA及其在激活基因表达中的应用
公开(公告)号：US5710031A
公开(公告)日：1998-01-20
申请号：US459174
申请日：1995-06-02
IPC分类号： A01N63/00 , C07K14/21 , C12N15/52 , C12N15/78 , C12N15/82 , C12P17/08 , C12P17/10 , C12N15/31 , C12N15/67
CPC分类号： C12R1/39 , A01N63/00 , C07K14/195 , C07K14/21 , C12N15/52 , C12N15/78 , C12N15/8282 , C12P17/08 , C12P17/10
摘要： Gene activating sequences which activate the expression of other bacterial genes, which are latent or expressed at low levels, are provided. The gene activating sequences confer the ability to produce several metabolites and may be transferred to bacterial strains. The transformed biocontrol agents are active to inhibit the growth of the fungal pathogens.
摘要翻译： 提供激活潜在或低水平表达的其他细菌基因表达的基因激活序列。 基因激活序列赋予产生几种代谢物的能力并且可以转移到细菌菌株中。 转化的生物防治剂有效地抑制真菌病原体的生长。
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3.发明授权Genetically modified Pseudomonas strains with enhanced biocontrol activity 失效具有增强的生物防治活性的遗传修饰的假单胞菌菌株
公开(公告)号：US5756087A
公开(公告)日：1998-05-26
申请号：US761258
申请日：1996-12-06
IPC分类号： A01N63/00 , C07K14/21 , C12N1/21 , C12N15/52 , C12N15/78 , C12P17/10 , C12P17/12 , C05F11/08
CPC分类号： C12R1/39 , A01N63/00 , C07K14/21 , C12N15/52 , C12N15/78 , C12P17/10 , C12P17/12 , Y10S435/876
摘要： Strains of Pseudomonas have been genetically engineered to have enhanced biocontrol properties. The strains of the invention are particularly effective against plant pathogenic fungi such as species of Rhizoctonia and Pythium, because the strains produce enhanced amounts of antifungal metabolites such as pyrrolnitrin that are active against these fungal pathogens. Both the genetically modified biocontrol strains and the antifungal metabolites can be used as active agents for biocontrol compositions.
摘要翻译： 已将基因工程改造为具有增强的生物防治性质的假单胞菌菌株。 本发明的菌株对于植物致病真菌如丝核菌属和腐霉属物种特别有效，因为菌株产生增加量的抗真菌代谢物，例如对这些真菌病原体具有活性的吡咯烷酮。 遗传修饰的生物防治菌株和抗真菌代谢物均可用作生物防治组合物的活性剂。
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4.发明授权Genomic DNA encoding a pseudomonas global transcriptional activation element and its use in activating gene expression 失效编码假单胞菌全基因转录激活因子的基因组DNA及其在激活基因表达中的应用
公开(公告)号：US5670350A
公开(公告)日：1997-09-23
申请号：US287442
申请日：1994-08-08
IPC分类号： A01N63/00 , C07K14/21 , C12N15/52 , C12N15/78 , C12N15/82 , C12P17/08 , C12P17/10 , C12N15/00 , C12N15/31 , C12N15/63
CPC分类号： C12R1/39 , A01N63/00 , C07K14/195 , C07K14/21 , C12N15/52 , C12N15/78 , C12N15/8282 , C12P17/08 , C12P17/10
摘要： Gene activating sequences which activate the expression of other bacterial genes, which are latent or expressed at low levels, are provided. The gene activating sequences confer the ability to produce several metabolites and may be transferred to bacterial strains. The transformed biocontrol agents are active to inhibit the growth of the fungal pathogens.
摘要翻译： 提供激活潜在或低水平表达的其他细菌基因表达的基因激活序列。 基因激活序列赋予产生几种代谢物的能力并且可以转移到细菌菌株中。 转化的生物防治剂有效地抑制真菌病原体的生长。
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公开(公告)号：US5610044A
公开(公告)日：1997-03-11
申请号：US346331
申请日：1994-11-29
摘要： The present invention relates to a method for enhancing colonization of desired microorganisms in a localized environment comprising introducing into said localized environment a genetically engineered microorganism comprising a gene comprising a DNA sequence coding for one or more substrate utilizing protein, wherein said substrate utilizing protein confers a competitive effective ability to utilize a substrate by a microorganism in which it is genetically engineered, as well as the isolated genes and the genetically engineered microorganisms of such a method.
摘要翻译： 本发明涉及一种在局部环境中增强所需微生物定植的方法，包括向所述局部环境中引入包含编码一种或多种底物利用蛋白质的DNA序列的基因的遗传工程微生物，其中所述利用蛋白质的底物赋予 通过其被遗传工程化的微生物利用底物的竞争有效能力，以及这种方法的分离的基因和遗传工程微生物。
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6.发明授权Genomic DNA encoding the pseudomonas fluorescens alternative sigma factor, RpoS, capable of activating gene expression 失效编码假单胞菌荧光素的替代西格玛因子的基因组DNA，能激活基因表达的RpoS
公开(公告)号：US5686283A
公开(公告)日：1997-11-11
申请号：US460298
申请日：1995-06-02
申请人： Thomas D. Gaffney , Stephen T. Lam
发明人： Thomas D. Gaffney , Stephen T. Lam
IPC分类号： A01N63/00 , C07K14/21 , C12N15/52 , C12N15/78 , C12N15/82 , C12P17/08 , C12P17/10 , C12N15/31 , C12N15/67
CPC分类号： C12R1/39 , A01N63/00 , C07K14/195 , C07K14/21 , C12N15/52 , C12N15/78 , C12N15/8282 , C12P17/08 , C12P17/10
摘要： Gene activating sequences which activate the expression of other bacterial genes, which are latent or expressed at low levels, are provided. The gene activating sequences confer the ability to produce several metabolites and may be transferred to bacterial strains. The transformed biocontrol agents are active to inhibit the growth of the fungal pathogens.
摘要翻译： 提供激活潜在或低水平表达的其他细菌基因表达的基因激活序列。 基因激活序列赋予产生几种代谢物的能力并且可以转移到细菌菌株中。 转化的生物防治剂有效地抑制真菌病原体的生长。
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7.发明授权DNA encoding the pseudomonas fluorescens sensor/kinase element, lemA, and its use in activating gene expression 失效编码荧光假单胞菌传感器/激酶元件的DNA，lemA及其在激活基因表达中的用途
公开(公告)号：US5686282A
公开(公告)日：1997-11-11
申请号：US459701
申请日：1995-06-02
申请人： Stephen T. Lam , Thomas D. Gaffney
发明人： Stephen T. Lam , Thomas D. Gaffney
IPC分类号： A01N63/00 , C07K14/21 , C12N15/52 , C12N15/78 , C12N15/82 , C12P17/08 , C12P17/10 , C12N15/00 , C12N15/31 , C12N15/63
CPC分类号： C12R1/39 , A01N63/00 , C07K14/195 , C07K14/21 , C12N15/52 , C12N15/78 , C12N15/8282 , C12P17/08 , C12P17/10
摘要： Gene activating sequences which activate the expression of other bacterial genes, which are latent or expressed at low levels, are provided. The gene activating sequences confer the ability to produce several metabolites and may be transferred to bacterial strains. The transformed biocontrol agents are active to inhibit the growth of the fungal pathogens.
摘要翻译： 提供激活潜在或低水平表达的其他细菌基因表达的基因激活序列。 基因激活序列赋予产生几种代谢物的能力并且可以转移到细菌菌株中。 转化的生物防治剂有效地抑制真菌病原体的生长。
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8.发明授权DNA encoding lemA-independent GacA and its use in activating gene expression 失效编码lemA的GacA的DNA及其在激活基因表达中的应用
公开(公告)号：US5891688A
公开(公告)日：1999-04-06
申请号：US9218
申请日：1998-01-20
申请人： Thomas D. Gaffney , Stephen T. Lam
发明人： Thomas D. Gaffney , Stephen T. Lam
IPC分类号： A01N63/00 , C07K14/21 , C12N1/21 , C12N15/31 , C12N15/52 , C12N15/78 , C12P17/10 , C12P17/12 , C12N15/63
摘要： The present invention exploits the discovery that certain single base changes in the gacA gene coding sequence, which result in certain single amino acid changes in the encoded GacA protein, render it LemA-independent. The present invention therefore provides DNA that encodes mutant GacA proteins that do not require phosphorylation by LemA in order to be active as transcriptional activators.
摘要翻译： 本发明利用这样的发现，即gacA基因编码序列中的某些单碱基变化导致编码的GacA蛋白中的某些单个氨基酸变化使其与LemA无关。 因此，本发明提供编码不需要LemA磷酸化以便作为转录激活子起作用的突变型GacA蛋白质的DNA。
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公开(公告)号：US5397697A
公开(公告)日：1995-03-14
申请号：US576259
申请日：1990-08-31
CPC分类号： C12N15/74 , C12N15/78 , C12Q1/6897
摘要： The invention is a method for identifying and cloning bacterial genes that are responsive to the presence of a plant host, comprising mutating a bacterial culture with a transposon containing a promoterless marker gene, exposing the bacterial mutants to root exudates, other plant-derived substances or plants, and identifying mutants that have a transposon-associated gene inducible by the plant. These mutants may be used to clone and characterize the promoters and the associated wild-type bacterial genes responsive to the plant.
摘要翻译： 本发明是用于鉴定和克隆对植物宿主的存在有反应的细菌基因的方法，包括用含有无启动子标记基因的转座子突变细菌培养物，将细菌突变体暴露于根系分泌物，其它植物衍生物质或 植物和识别具有植物诱导的转座子相关基因的突变体。 这些突变体可用于克隆和表征对植物有反应的启动子和相关的野生型细菌基因。
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