公开(公告)号：US5798215A

公开(公告)日：1998-08-25

申请号：US420987

申请日：1995-04-06

申请人： Cheryl A. Cathey ,  Tom Saul ,  Nicole D. Bloom ,  Hans O. Ribi ,  Henry L. Schwartz ,  Jeffrey B. Langford ,  David J. Paul

发明人： Cheryl A. Cathey ,  Tom Saul ,  Nicole D. Bloom ,  Hans O. Ribi ,  Henry L. Schwartz ,  Jeffrey B. Langford ,  David J. Paul

IPC分类号： G01N31/00 ,  B01F13/00 ,  B01F13/08 ,  B01L3/00 ,  B01L7/00 ,  G01N21/78 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543 ,  G01N35/00 ,  G01N33/558

CPC分类号： G01N33/54366 ,  B01F13/0059 ,  B01F13/0818 ,  B01L3/5027 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0677 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  G01N2035/00267 ,  Y10S435/81 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/81

摘要： A device and method for its use in diagnostic assays are provided. The device comprises at least one assay path, where an assay path includes a main flow path and at least one side reagent channel. The main flow path begins at a sample addition port, continues through a main reagent area into an incubation area and ends in a waste area. In fluid communication with the main flow path is at least one side reagent channel that begins at a liquid addition port, continues through a side reagent area and ends in said main channel at a region between the main reagent and waste areas, usually upstream from the incubation area. Agitation means may be included in at least one of the main and side reagent areas and/or the incubation area. At least one fluid interruption means located at various positions along the main and side reagent channels upstream from the incubation area provide for control over reagent interaction and fluid flow through the device. Applications in which the device finds use include diagnostic assays employing signal producing systems based on the interactions of specific binding pair members, where an optical signal is related to the presence of analyte.

摘要翻译： 提供了一种用于诊断测定的装置和方法。 该装置包括至少一个测定路径，其中测定路径包括主流路径和至少一个侧试剂通道。 主流路开始于样品添加口，继续通过主试剂区进入孵育区，并结束于废物区。 与主流路流体连通是至少一个从液体添加口开始的侧试剂通道，通过侧试剂区域继续并在主试剂和废物区域之间的区域中终止于所述主通道，通常在 孵化区。 搅拌装置可以包括在主试剂区域和侧试剂区域和/或孵化区域中的至少一个中。 位于沿孵化区域上游的主要和侧面试剂通道的不同位置处的至少一个流体中断装置提供对通过装置的试剂相互作用和流体流动的控制。 设备使用的应用包括基于特定结合对成员的相互作用的信号产生系统的诊断测定，其中光学信号与分析物的存在相关。

公开(公告)号：US5660993A

公开(公告)日：1997-08-26

申请号：US296489

申请日：1994-08-24

申请人： Cheryl A. Cathey ,  Tom Saul ,  Nicole D. Bloom ,  Hans O. Ribi ,  Henry L. Schwartz ,  Jeffrey B. Langford

发明人： Cheryl A. Cathey ,  Tom Saul ,  Nicole D. Bloom ,  Hans O. Ribi ,  Henry L. Schwartz ,  Jeffrey B. Langford

IPC分类号： G01N33/48 ,  B01F13/00 ,  B01F13/08 ,  B01L3/00 ,  C12M1/34 ,  C12Q1/00 ,  G01N33/52 ,  G01N33/543 ,  G01N35/00 ,  G01N33/558

CPC分类号： B01L3/50273 ,  B01F13/0059 ,  B01F13/0818 ,  B01L3/5027 ,  B01L3/502746 ,  G01N33/54366 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0406 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L3/502738 ,  G01N2035/00267 ,  Y10S435/81 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/81

摘要： A disposable diagnostic assay device and method for its use are provided. The device comprises a sample addition port in fluid communication with at least one main channel. The main channel comprises, in the direction of fluid flow, a main reagent area in fluid communication with an incubation area and a waste area. In fluid communication with the main channel is at least one side reagent channel. The side reagent channels comprise, in the direction of fluid flow, a liquid addition port and a side reagent area in fluid communication with the main channel at a region of the main channel upstream from the incubation area. Agitation means may be included in the at least one of the main and side reagent areas and/or the incubation area. Capillary valves may be located at various positions along the main and side reagent channels upstream from the incubation area, providing for control over fluid flow through the device. Applications in which the device finds use include diagnostic assays employing signal producing systems based on the interactions of specific binding pair members, where optical signals are related to the presence of analyte.

摘要翻译： 提供了一种用于其使用的一次性诊断测定装置和方法。 该装置包括与至少一个主通道流体连通的样品加入口。 主流道在流体流动的方向上包括与孵育区域和废物区域流体连通的主试剂区域。 与主通道流体连通是至少一个侧试剂通道。 侧试剂通道在流体流动的方向上包括在孵育区域上游的主通道的区域处与主通道流体连通的液体添加口和侧试剂区域。 搅拌装置可以包括在主试剂区域和侧试剂区域和/或孵育区域中的至少一个中。 毛细管阀可以位于沿着孵化区域上游的主要和侧面试剂通道的不同位置，从而控制通过该装置的流体流动。 设备使用的应用包括基于特定结合对成员的相互作用的信号产生系统的诊断测定，其中光学信号与分析物的存在相关。

公开(公告)号：US5503985A

公开(公告)日：1996-04-02

申请号：US179749

申请日：1994-01-07

申请人： Cheryl A. Cathey ,  Henry L. Schwartz ,  Tom Saul ,  Jeffrey D. Langford

发明人： Cheryl A. Cathey ,  Henry L. Schwartz ,  Tom Saul ,  Jeffrey D. Langford

IPC分类号： G01N33/48 ,  B01F13/00 ,  B01F13/08 ,  B01L3/00 ,  C12M1/34 ,  C12Q1/00 ,  G01N33/52 ,  G01N33/543 ,  G01N35/00 ,  G01N33/542 ,  G01N33/558

CPC分类号： B01L3/50273 ,  B01F13/0059 ,  B01F13/0818 ,  B01L3/5027 ,  B01L3/502746 ,  G01N33/54366 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0406 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L3/502738 ,  G01N2035/00267 ,  Y10S435/81 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/81

摘要： A disposable diagnostic device and method of its use are provided. The device comprises a housing containing first and second flow paths orthogonal to each other. The first flow path commences at a sample addition port and continues through a transport channel which feeds sample to an incubation area by means of capillary flow. The incubation area comprises a signal producing system and is underneath an optically-clear window. The first flow path terminates in a top waste reservoir which receives sample and wash fluid. The second flow path begins on one side of the incubation area at an inlet port over a side reagent reservoir. Liquid flows along the second flow path from the side reagent reservoir across the incubation area into the side waste reservoir. The incubation area may comprise agitation means for homogenous dispersion of reagent into liquid. Various reagents of a signal producing system may be contained within the device and the necessary liquids added automatically by appropriate instrumentation, so as to have the assay carried out automatically, without technician involvement, providing an accurate and sensitive determination.

摘要翻译： 提供了一次性诊断装置及其使用方法。 该装置包括容纳彼此正交的第一和第二流动路径的壳体。 第一流路在样品加入口处开始并继续通过运送通道，其通过毛细管流将样品送入孵育区。 孵化区包括信号产生系统，并在光学透明的窗口下方。 第一流路终止于接收样品和洗涤流体的顶部废物储存器中。 第二流路在侧试剂储存器的入口处从孵化区的一侧开始。 液体沿着第二流动路径从侧面试剂储存器流过孵化区域流入侧面废物储存器。 孵育区域可以包括用于将试剂均匀分散到液体中的搅拌装置。 信号产生系统的各种试剂可以包含在装置内，并且通过适当的仪器自动加入必需的液体，以便在没有技术人员参与的情况下自动进行测定，提供精确和灵敏的测定。

公开(公告)号：US5698406A

公开(公告)日：1997-12-16

申请号：US414331

申请日：1995-03-30

申请人： Cheryl A. Cathey ,  Henry L. Schwartz ,  Tom Saul ,  Jeffrey D. Langford

发明人： Cheryl A. Cathey ,  Henry L. Schwartz ,  Tom Saul ,  Jeffrey D. Langford

IPC分类号： G01N33/48 ,  B01F13/00 ,  B01F13/08 ,  B01L3/00 ,  C12M1/34 ,  C12Q1/00 ,  G01N33/52 ,  G01N33/543 ,  G01N35/00 ,  G01N33/53 ,  G01N33/558

CPC分类号： B01L3/50273 ,  B01F13/0059 ,  B01F13/0818 ,  B01L3/5027 ,  B01L3/502746 ,  G01N33/54366 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0406 ,  B01L2400/0688 ,  B01L2400/086 ,  B01L3/502738 ,  G01N2035/00267 ,  Y10S435/81 ,  Y10S435/973 ,  Y10S436/805 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/81

摘要： A disposable diagnostic device and method of its use are provided. The device comprises a housing containing first and second flow paths orthogonal to each other. The first flow path commences at a sample addition port and continues through a transport channel which feeds sample to an incubation area by means of capillary flow. The incubation area comprises a signal producing system and is underneath an optically-clear window. The first flow path terminates in a top waste reservoir which receives sample and wash fluid. The second flow path begins on one side of the incubation area at an inlet port over a side reagent reservoir. Liquid flows along the second flow path from the side reagent reservoir across the incubation area into the side waste reservoir. The incubation area may comprise agitation means for homogenous dispersion of reagent into liquid. Various reagents of a signal producing system may be contained within the device and the necessary liquids added automatically by appropriate instrumentation, so as to have the assay carried out automatically, without technician involvement, providing an accurate and sensitive determination.

摘要翻译： 提供了一次性诊断装置及其使用方法。 该装置包括容纳彼此正交的第一和第二流动路径的壳体。 第一流路在样品加入口处开始并继续通过运送通道，其通过毛细管流将样品送入孵育区。 孵化区包括信号产生系统，并在光学透明的窗口下方。 第一流路终止于接收样品和洗涤流体的顶部废物储存器中。 第二流路在侧试剂储存器的入口处从孵化区的一侧开始。 液体沿着第二流动路径从侧面试剂储存器流过孵化区域流入侧面废物储存器。 孵育区域可以包括用于将试剂均匀分散到液体中的搅拌装置。 信号产生系统的各种试剂可以包含在装置内，并且通过适当的仪器自动加入必需的液体，以便在没有技术人员参与的情况下自动进行测定，提供精确和灵敏的测定。

公开(公告)号：US5399486A

公开(公告)日：1995-03-21

申请号：US19469

申请日：1993-02-18

申请人： Cheryl A. Cathey ,  Henry L. Schwartz

发明人： Cheryl A. Cathey ,  Henry L. Schwartz

IPC分类号： B01L3/00 ,  G01N33/543 ,  G01N35/00 ,  C12Q1/00 ,  G01N33/53 ,  G01N33/566

CPC分类号： B01L3/50273 ,  G01N33/54366 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2400/0406 ,  B01L2400/086 ,  B01L3/502738 ,  B01L3/502746 ,  G01N2035/00267

摘要： A disposable diagnostic unit is provided which employs a housing which provides for a sample port, and a channel which feeds the sample to an incubation area by means of capillary action. The incubation area is underneath an optically-clear window and comprises a lipid membrane which has optical properties, particularly fluorescent properties and usually a reagent. A reservoir at the end of the channel downstream from the incubation area receives the sample and waste washes, while on one side of the platform area is a reagent reservoir and on the other side a side waste reservoir, so that one can move the reagent from the reagent reservoir through the platform area into the waste reservoir. Various reagents may be contained within the unit and the necessary liquids added automatically by appropriate instrumentation, so as to have the assay carried out automatically, without technician involvement, providing an accurate and sensitive determination.

摘要翻译： 提供了一种一次性诊断单元，其使用提供样品端口的壳体和通过毛细管作用将样品送入孵育区的通道。 孵育区域在光学透明窗口的下方，并且包括具有光学性质，特别是荧光性质并且通常是试剂的脂质膜。 在孵化区下游的通道末端的储存器接收样品和废物洗涤，而在平台区域的一侧是试剂储存器，而在另一侧具有侧面废物储存器，使得可以将试剂从 试剂库通过平台区域进入废水库。 可以在单元内包含各种试剂，并通过适当的仪器自动加入必需的液体，以便在没有技术人员参与的情况下自动进行测定，提供准确和灵敏的测定。