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公开(公告)号：US5258497A
公开(公告)日：1993-11-02
申请号：US552172
申请日：1990-07-13
IPC分类号： C12P21/00 , C07K1/16 , C07K14/005 , C07K14/195 , C07K14/47 , C12R1/19 , C07K3/02 , C07K15/06 , C07K15/16
CPC分类号： C07K14/4721
摘要： The present invention relates to processes for purifying annexines. The overall process includes the steps of preparing a homogenized cell preparation and adjusting the pH to about 8.0 to 10.0, adding at least one bivalent cation, adding a phospholipid, washing the insoluble cell residue to remove the soluble constituents, and extracting the annexines from the cell residue with a chelating agent. This process initially promotes the adsorption of the annexines onto the insoluble cell residue or membrane of the host organism. The adsorption step makes it possible to eliminate unwanted soluble matter from the homogenized material by simple washing. Desorption is accomplished by using a chelating agent.
摘要翻译： 本发明涉及净化附睾的方法。 整个方法包括以下步骤：制备均匀的细胞制剂并将pH调节至约8.0至10.0，加入至少一种二价阳离子，加入磷脂，洗涤不溶性细胞残渣以除去可溶性成分，并从 细胞残留物与螯合剂。 该过程最初促进附着物吸附到宿主生物体的不溶性细胞残留物或膜上。 吸附步骤可以通过简单的洗涤从均质材料中除去不需要的可溶物质。 通过使用螯合剂来完成解吸。
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公开(公告)号：US5955437A
公开(公告)日：1999-09-21
申请号：US445859
申请日：1995-05-22
CPC分类号： A61K49/0056 , A61K49/0043 , A61K51/08 , A61K51/087 , C07K14/4721 , G01N33/86 , G01N33/92 , A61K2123/00 , G01N2333/4718 , G01N2405/04 , Y10S435/81 , Y10S435/968 , Y10S436/801 , Y10S436/804 , Y10S436/821 , Y10T436/105831 , Y10T436/107497
摘要： Detectably labelled annexines and compositions thereof are disclosed. Also disclosed are methods for diagnosing a disruption or activation of the hemostatic system or a prothrombotic state in an individual suspected of having a hemostatic disorder, by contacting the blood of said individual with an annexine, and detecting whether an annexine-platelet complex is formed.
摘要翻译： 公开了可检测标记的膜蛋白及其组合物。 还公开了通过使所述个体的血液与膜联接并检测是否形成膜状血小板复合物来诊断疑似患有止血障碍的个体中的止血系统的破坏或活化或血栓前状态的方法。
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公开(公告)号：US5627036A
公开(公告)日：1997-05-06
申请号：US183788
申请日：1994-01-21
CPC分类号： A61K49/0056 , A61K49/0043 , A61K51/08 , A61K51/087 , C07K14/4721 , G01N33/86 , G01N33/92 , A61K2123/00 , G01N2333/4718 , G01N2405/04 , Y10S435/81 , Y10S435/968 , Y10S436/801 , Y10S436/804 , Y10S436/821 , Y10T436/105831 , Y10T436/107497
摘要： Detectably labelled annexines and compositions thereof are disclosed. Also disclosed are methods for diagnosing a disruption or activation of the hemostatic system or a prothrombotic state in an individual suspected of having a hemostatic disorder, by contacting the blood of said individual with an annexine, and detecting whether an annexine-platelet complex is formed.
摘要翻译： 公开了可检测标记的膜蛋白及其组合物。 还公开了通过使所述个体的血液与膜联接并检测是否形成膜状血小板复合物来诊断疑似患有止血障碍的个体中的止血系统的破坏或活化或血栓前状态的方法。
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公开(公告)号：US5296467A
公开(公告)日：1994-03-22
申请号：US873601
申请日：1992-04-21
CPC分类号： A61K38/1709
摘要： Pharmaceutical compositions which consist essentially of a vascular anticoagulant annexine, Ca.sup.2+, Zn.sup.2+, and a pharmaceutically acceptable carrier are disclosed. Also disclosed are processes for preparing such pharmaceutical compositions. Also disclosed are methods of preventing the coagulation of blood by mixing the blood with a vascular anticoagulant annexine, Ca.sup.2+ and Zn.sup.2+.
摘要翻译： 公开了基本上由血管抗凝剂annexine，Ca2 +，Zn2 +和药学上可接受的载体组成的药物组合物。 还公开了制备这种药物组合物的方法。 还公开了通过将血液与血管抗凝剂annexine，Ca2 +和Zn2 +混合来防止血液凝结的方法。
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